Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury
Background: Burn injuries are an alarming indicator of the sensitivity of human tissue when confronted with high temperatures or chemicals. The current treatment for burn wounds needs to be improved and more extensive in scope. Significant research advances concerning the therapeutic potential of s...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Tripoli University
2023-12-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | https://www.openveterinaryjournal.com/?mno=165991 |
| _version_ | 1797357198714601472 |
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| author | Mavheena Moghan Paarvaishnee Naidu Nadhrah binti Zulkifly Yulfia Nelymalik Selan Erif Maha Nugraha Setyawan Sugi Winarsih Dwi Liliek Kusindarta |
| author_facet | Mavheena Moghan Paarvaishnee Naidu Nadhrah binti Zulkifly Yulfia Nelymalik Selan Erif Maha Nugraha Setyawan Sugi Winarsih Dwi Liliek Kusindarta |
| author_sort | Mavheena Moghan |
| collection | DOAJ |
| description | Background:
Burn injuries are an alarming indicator of the sensitivity of human tissue when confronted with high temperatures or chemicals. The current treatment for burn wounds needs to be improved and more extensive in scope. Significant research advances concerning the therapeutic potential of secretomes over the past two decades have expanded the range of therapies that utilize secretomes to encompass populations other than stem cells.
Aim:
This study details how the secretome extracted from the Bovine Umbilical Vein Endothelial Cell (BUVEC) promotes the healing of burn injuries.
Methods:
The 48 rats were divided into four groups, namely the control group with povidone-iodine, the 5% BUVEC-CM cream group, the 10% BUVEC-CM cream group, the 15% BUVEC-CM cream group. Animals induced type II burns under anesthesia. Treatment is carried out topically, 2 times a day. Every day the wound was measured. The animals were put to sleep for samples on days 5, 13, 21, and 19. Samples in the form of skins were soaked in 4% paraformaldehyde and processed with paraffin-embedded for tissue preparations. The research results were processed using Two Ways ANOVA.
Results:
The study showed that on day 5, wound closure occurred, whereas in the povidone-iodine group, macroscopically, the wound closed faster. Epithelial repair, increased fibroblasts and collagen, and blood vessel formation greatly increased in the 15% BUVEC-CM group on days 13, 21, and 29.
Conclusion:
Taken together, BUVEC secretome promoted fibroblast regeneration, collagen formation, re-epithelialization, and hair follicle regeneration on the burn injury wound healing. [Open Vet J 2023; 13(12.000): 1597-1606] |
| first_indexed | 2024-03-08T14:40:22Z |
| format | Article |
| id | doaj.art-f9f5bb0d24da49aeb9e0ba6a8c773780 |
| institution | Directory Open Access Journal |
| issn | 2226-4485 2218-6050 |
| language | English |
| last_indexed | 2024-03-08T14:40:22Z |
| publishDate | 2023-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-f9f5bb0d24da49aeb9e0ba6a8c7737802024-01-11T18:57:11ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502023-12-0113121597160610.5455/OVJ.2023.v13.i12.9165991Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injuryMavheena Moghan0Paarvaishnee Naidu1Nadhrah binti Zulkifly2Yulfia Nelymalik Selan3Erif Maha Nugraha Setyawan4Sugi Winarsih5Dwi Liliek Kusindarta6Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia. Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia. Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia. Department of Anatomy, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Nusa Tenggara Timur, Indonesia Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia, 55281 Department of Agriculture, Food, and Fisheries of Sleman Regency, Yogyakarta 55281, Indonesia Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.Background: Burn injuries are an alarming indicator of the sensitivity of human tissue when confronted with high temperatures or chemicals. The current treatment for burn wounds needs to be improved and more extensive in scope. Significant research advances concerning the therapeutic potential of secretomes over the past two decades have expanded the range of therapies that utilize secretomes to encompass populations other than stem cells. Aim: This study details how the secretome extracted from the Bovine Umbilical Vein Endothelial Cell (BUVEC) promotes the healing of burn injuries. Methods: The 48 rats were divided into four groups, namely the control group with povidone-iodine, the 5% BUVEC-CM cream group, the 10% BUVEC-CM cream group, the 15% BUVEC-CM cream group. Animals induced type II burns under anesthesia. Treatment is carried out topically, 2 times a day. Every day the wound was measured. The animals were put to sleep for samples on days 5, 13, 21, and 19. Samples in the form of skins were soaked in 4% paraformaldehyde and processed with paraffin-embedded for tissue preparations. The research results were processed using Two Ways ANOVA. Results: The study showed that on day 5, wound closure occurred, whereas in the povidone-iodine group, macroscopically, the wound closed faster. Epithelial repair, increased fibroblasts and collagen, and blood vessel formation greatly increased in the 15% BUVEC-CM group on days 13, 21, and 29. Conclusion: Taken together, BUVEC secretome promoted fibroblast regeneration, collagen formation, re-epithelialization, and hair follicle regeneration on the burn injury wound healing. [Open Vet J 2023; 13(12.000): 1597-1606]https://www.openveterinaryjournal.com/?mno=165991secretomebuvecburn woundrat modelremodeling |
| spellingShingle | Mavheena Moghan Paarvaishnee Naidu Nadhrah binti Zulkifly Yulfia Nelymalik Selan Erif Maha Nugraha Setyawan Sugi Winarsih Dwi Liliek Kusindarta Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury Open Veterinary Journal secretome buvec burn wound rat model remodeling |
| title | Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury |
| title_full | Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury |
| title_fullStr | Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury |
| title_full_unstemmed | Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury |
| title_short | Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury |
| title_sort | secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury |
| topic | secretome buvec burn wound rat model remodeling |
| url | https://www.openveterinaryjournal.com/?mno=165991 |
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