The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of Toxicity
Fenpropathrin, a pyrethroid insecticide, has been widely used for many years in agricultural fields. It works by disturbing the voltage-gated sodium channel, leading to paralysis and the death of the target animal. While past studies have focused on neurodegeneration following fenpropathrin poisonin...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2079-7737/12/9/1214 |
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| author | Ferry Saputra Yu-Heng Lai Marri Jmelou M. Roldan Honeymae C. Alos Charlaine A. Aventurado Ross D. Vasquez Chung-Der Hsiao |
| author_facet | Ferry Saputra Yu-Heng Lai Marri Jmelou M. Roldan Honeymae C. Alos Charlaine A. Aventurado Ross D. Vasquez Chung-Der Hsiao |
| author_sort | Ferry Saputra |
| collection | DOAJ |
| description | Fenpropathrin, a pyrethroid insecticide, has been widely used for many years in agricultural fields. It works by disturbing the voltage-gated sodium channel, leading to paralysis and the death of the target animal. While past studies have focused on neurodegeneration following fenpropathrin poisoning in humans, relatively few pieces of research have examined its effect on other peripheral organs. This study successfully investigated the potential toxicity of fenpropathrin on the cardiovascular system using zebrafish as an animal model. Zebrafish larvae exposed to varying doses of fenpropathrin underwent an evaluation of cardiac physiology by measuring the heart rate, stroke volume, cardiac output, and shortening fraction. The blood flow velocity and the dorsal aorta diameter were also measured to assess the impact of fenpropathrin exposure on the vascular system. Furthermore, molecular docking was performed to evaluate the pesticide binding affinity to various proteins associated with the cardiovascular system, revealing the potential mechanism of the fenpropathrin cardiotoxic effect. The findings demonstrated a significant dose-dependent increase in the heart rate stroke volume, cardiac output, shortening fraction, and ejection fraction of zebrafish larvae after 24 h of acute treatment with fenpropathrin. Additionally, zebrafish treated at a concentration of 1 ppm exhibited significantly larger blood vessels in diameter and an increased blood flow velocity compared to the control group. According to molecular docking, fenpropathrin showed a high affinity for various voltage-gated sodium channels like scn1lab, cacna1sb, and clcn3. Finally, from the results, we found that fenpropathrin caused cardiomegaly, which may have been induced by the voltage-gated sodium channel disruption. This study highlights the significant disruption of fenpropathrin in the cardiovascular system and emphasizes the need for further research on the health implications of this pesticide. |
| first_indexed | 2024-03-10T23:01:59Z |
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| language | English |
| last_indexed | 2024-03-10T23:01:59Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-f9f8d09826cf4080b75253c82a059ae32023-11-19T09:38:38ZengMDPI AGBiology2079-77372023-09-01129121410.3390/biology12091214The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of ToxicityFerry Saputra0Yu-Heng Lai1Marri Jmelou M. Roldan2Honeymae C. Alos3Charlaine A. Aventurado4Ross D. Vasquez5Chung-Der Hsiao6Department of Chemistry, Chung Yuan Christian University, Taoyuan 320314, TaiwanDepartment of Chemistry, Chinese Culture University, Taipei 11114, TaiwanThe Graduate School, University of Santo Tomas, Manila 1008, PhilippinesThe Graduate School, University of Santo Tomas, Manila 1008, PhilippinesThe Graduate School, University of Santo Tomas, Manila 1008, PhilippinesThe Graduate School, University of Santo Tomas, Manila 1008, PhilippinesDepartment of Chemistry, Chung Yuan Christian University, Taoyuan 320314, TaiwanFenpropathrin, a pyrethroid insecticide, has been widely used for many years in agricultural fields. It works by disturbing the voltage-gated sodium channel, leading to paralysis and the death of the target animal. While past studies have focused on neurodegeneration following fenpropathrin poisoning in humans, relatively few pieces of research have examined its effect on other peripheral organs. This study successfully investigated the potential toxicity of fenpropathrin on the cardiovascular system using zebrafish as an animal model. Zebrafish larvae exposed to varying doses of fenpropathrin underwent an evaluation of cardiac physiology by measuring the heart rate, stroke volume, cardiac output, and shortening fraction. The blood flow velocity and the dorsal aorta diameter were also measured to assess the impact of fenpropathrin exposure on the vascular system. Furthermore, molecular docking was performed to evaluate the pesticide binding affinity to various proteins associated with the cardiovascular system, revealing the potential mechanism of the fenpropathrin cardiotoxic effect. The findings demonstrated a significant dose-dependent increase in the heart rate stroke volume, cardiac output, shortening fraction, and ejection fraction of zebrafish larvae after 24 h of acute treatment with fenpropathrin. Additionally, zebrafish treated at a concentration of 1 ppm exhibited significantly larger blood vessels in diameter and an increased blood flow velocity compared to the control group. According to molecular docking, fenpropathrin showed a high affinity for various voltage-gated sodium channels like scn1lab, cacna1sb, and clcn3. Finally, from the results, we found that fenpropathrin caused cardiomegaly, which may have been induced by the voltage-gated sodium channel disruption. This study highlights the significant disruption of fenpropathrin in the cardiovascular system and emphasizes the need for further research on the health implications of this pesticide.https://www.mdpi.com/2079-7737/12/9/1214fenpropathrinzebrafishcardiovascular toxicitypesticide |
| spellingShingle | Ferry Saputra Yu-Heng Lai Marri Jmelou M. Roldan Honeymae C. Alos Charlaine A. Aventurado Ross D. Vasquez Chung-Der Hsiao The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of Toxicity Biology fenpropathrin zebrafish cardiovascular toxicity pesticide |
| title | The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of Toxicity |
| title_full | The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of Toxicity |
| title_fullStr | The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of Toxicity |
| title_full_unstemmed | The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of Toxicity |
| title_short | The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of Toxicity |
| title_sort | effect of the pyrethroid pesticide fenpropathrin on the cardiac performance of zebrafish and the potential mechanism of toxicity |
| topic | fenpropathrin zebrafish cardiovascular toxicity pesticide |
| url | https://www.mdpi.com/2079-7737/12/9/1214 |
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