Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expression
Myostatin (MSTN) is a negative regulator of muscle growth, which restrains the proliferation and differentiation of myoblasts. To understand the role of two mstn genes of Takifugu bimaculatus, the full-length cDNAs of 1131 bp Tbmstn1 and 1,080 bp Tbmstn2 were obtained from the T. bimaculatus’ genomi...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2020-08-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/9655.pdf |
| _version_ | 1797424221662478336 |
|---|---|
| author | Yinzhen Sheng Yulong Sun Xin Zhang Haifu Wan Chengjie Yao Keying Liang Leibin Li Bo Liu Jianxing Zhong Ziping Zhang Yilei Wang |
| author_facet | Yinzhen Sheng Yulong Sun Xin Zhang Haifu Wan Chengjie Yao Keying Liang Leibin Li Bo Liu Jianxing Zhong Ziping Zhang Yilei Wang |
| author_sort | Yinzhen Sheng |
| collection | DOAJ |
| description | Myostatin (MSTN) is a negative regulator of muscle growth, which restrains the proliferation and differentiation of myoblasts. To understand the role of two mstn genes of Takifugu bimaculatus, the full-length cDNAs of 1131 bp Tbmstn1 and 1,080 bp Tbmstn2 were obtained from the T. bimaculatus’ genomic database, which encodes 376 and 359 amino acids, respectively. The results of qRT-PCR showed that Tbmstn1 was expressed in the eye, kidney, spleen, skeletal muscle, gill, and brain, and the expression level in the skeletal muscle was extremely significantly higher than in other examined tissues. Tbmstn2 was expressed in the skin, skeletal muscle, gill, and brain, and had the highest expression in the skeletal muscle, followed by expression in the brain. Meanwhile, in different stages of embryonic development, the expression of Tbmstn1 started from the gastrula stage. Its expression in the eye-pigment formation stage and hatching stage was significantly higher than that in other stages. The Tbmstn2 was expressed in all examined embryonic stages with different levels, and the highest expression was detected in the eye-pigment formation stage. These results suggested that Tbmstn1 and Tbmstn2 may involve in the development of skeletal muscle, and Tbmstn2 may be related to the formation of nervous system. |
| first_indexed | 2024-03-09T07:59:12Z |
| format | Article |
| id | doaj.art-f9f986b5070a477f8db1c569371ced04 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:59:12Z |
| publishDate | 2020-08-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-f9f986b5070a477f8db1c569371ced042023-12-03T00:50:47ZengPeerJ Inc.PeerJ2167-83592020-08-018e965510.7717/peerj.9655Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expressionYinzhen Sheng0Yulong Sun1Xin Zhang2Haifu Wan3Chengjie Yao4Keying Liang5Leibin Li6Bo Liu7Jianxing Zhong8Ziping Zhang9Yilei Wang10Fisheries College, Jimei University, Xiamen, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaFisheries College, Jimei University, Xiamen, ChinaFisheries College, Jimei University, Xiamen, ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaFisheries Research Institute of Fujian, Xiamen, P.R. ChinaFisheries Research Institute of Fujian, Xiamen, P.R. ChinaFisheries Research Institute of Fujian, Xiamen, P.R. ChinaCollege of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaFisheries College, Jimei University, Xiamen, ChinaMyostatin (MSTN) is a negative regulator of muscle growth, which restrains the proliferation and differentiation of myoblasts. To understand the role of two mstn genes of Takifugu bimaculatus, the full-length cDNAs of 1131 bp Tbmstn1 and 1,080 bp Tbmstn2 were obtained from the T. bimaculatus’ genomic database, which encodes 376 and 359 amino acids, respectively. The results of qRT-PCR showed that Tbmstn1 was expressed in the eye, kidney, spleen, skeletal muscle, gill, and brain, and the expression level in the skeletal muscle was extremely significantly higher than in other examined tissues. Tbmstn2 was expressed in the skin, skeletal muscle, gill, and brain, and had the highest expression in the skeletal muscle, followed by expression in the brain. Meanwhile, in different stages of embryonic development, the expression of Tbmstn1 started from the gastrula stage. Its expression in the eye-pigment formation stage and hatching stage was significantly higher than that in other stages. The Tbmstn2 was expressed in all examined embryonic stages with different levels, and the highest expression was detected in the eye-pigment formation stage. These results suggested that Tbmstn1 and Tbmstn2 may involve in the development of skeletal muscle, and Tbmstn2 may be related to the formation of nervous system.https://peerj.com/articles/9655.pdfmstn1mstn2SequenceGenomic structureExpression patternTakifugu bimaculatus |
| spellingShingle | Yinzhen Sheng Yulong Sun Xin Zhang Haifu Wan Chengjie Yao Keying Liang Leibin Li Bo Liu Jianxing Zhong Ziping Zhang Yilei Wang Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expression PeerJ mstn1 mstn2 Sequence Genomic structure Expression pattern Takifugu bimaculatus |
| title | Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expression |
| title_full | Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expression |
| title_fullStr | Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expression |
| title_full_unstemmed | Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expression |
| title_short | Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expression |
| title_sort | characterization of two myostatin genes in pufferfish takifugu bimaculatus sequence genomic structure and expression |
| topic | mstn1 mstn2 Sequence Genomic structure Expression pattern Takifugu bimaculatus |
| url | https://peerj.com/articles/9655.pdf |
| work_keys_str_mv | AT yinzhensheng characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT yulongsun characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT xinzhang characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT haifuwan characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT chengjieyao characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT keyingliang characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT leibinli characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT boliu characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT jianxingzhong characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT zipingzhang characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression AT yileiwang characterizationoftwomyostatingenesinpufferfishtakifugubimaculatussequencegenomicstructureandexpression |