Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia [version 2; peer review: 2 approved]
Candida krusei is a Candida non-albicans species with a high prevalence, which causes candidaemia. Current treatment guidelines include fluconazole as a primary therapeutic option for the treatment of these infections; however, it is only a fungistatic against Candida spp., and both inherent and acq...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
F1000 Research Ltd
2023-03-01
|
| Series: | F1000Research |
| Subjects: | |
| Online Access: | https://f1000research.com/articles/11-593/v2 |
| _version_ | 1797787437948207104 |
|---|---|
| author | Fadilah Fadilah Khaerunissa Anbar Istiadi Ayu Eka Fatril Linda Erlina Retno Wahyuningsih Anna Rozaliyani Rafika Indah Paramita Robiatul Adawiyah |
| author_facet | Fadilah Fadilah Khaerunissa Anbar Istiadi Ayu Eka Fatril Linda Erlina Retno Wahyuningsih Anna Rozaliyani Rafika Indah Paramita Robiatul Adawiyah |
| author_sort | Fadilah Fadilah |
| collection | DOAJ |
| description | Candida krusei is a Candida non-albicans species with a high prevalence, which causes candidaemia. Current treatment guidelines include fluconazole as a primary therapeutic option for the treatment of these infections; however, it is only a fungistatic against Candida spp., and both inherent and acquired resistance to fluconazole have been reported. C. krusei species is also reported as the only Candida sp. which has an intrinsic resistance factor to fluconazole. Therefore, in dealing with antifungal resistance, it is necessary to develop new antifungal agents that are efficient in the treatment of fungal infections, especially those caused by C. krusei. The purpose of this study was to investigate the genome of clinical C. krusei isolates and correlate the resistant phenotypes with mutations in resistance genes. A total of 16 samples of C. krusei from clinical samples from hospitals in Jakarta were used in the experiment. All colonies were extracted using the QIAamp DNA Mini Kit. The library was prepared using the Illumina DNA Prep Kit. The sequencing process was carried out on the Illumina MiSeq Platform using a 2x301 paired-end configuration. FASTQ raw files are available under the BioProject Accession Number PRJNA819536 and Sequence Read Archive Accession Numbers SRR18739949 and SRR18739964. |
| first_indexed | 2024-03-13T01:21:54Z |
| format | Article |
| id | doaj.art-f9fbccdd49ff479f9aec77a08d615c19 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-03-13T01:21:54Z |
| publishDate | 2023-03-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-f9fbccdd49ff479f9aec77a08d615c192023-07-05T00:00:00ZengF1000 Research LtdF1000Research2046-14022023-03-0111143748Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia [version 2; peer review: 2 approved]Fadilah Fadilah0https://orcid.org/0000-0002-8120-3138Khaerunissa Anbar Istiadi1Ayu Eka Fatril2Linda Erlina3Retno Wahyuningsih4Anna Rozaliyani5Rafika Indah Paramita6Robiatul Adawiyah7https://orcid.org/0000-0002-4987-4839Department of Medical Chemistry, , Faculty of Medicine, Universitas Indonesia,, Jakarta, Indonesia, 10430, IndonesiaBioinformatics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, IndonesiaDepartment of Medical Chemistry, , Faculty of Medicine, Universitas Indonesia,, Jakarta, Indonesia, 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, IndonesiaDepartment of Medical Chemistry, , Faculty of Medicine, Universitas Indonesia,, Jakarta, Indonesia, 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, IndonesiaCandida krusei is a Candida non-albicans species with a high prevalence, which causes candidaemia. Current treatment guidelines include fluconazole as a primary therapeutic option for the treatment of these infections; however, it is only a fungistatic against Candida spp., and both inherent and acquired resistance to fluconazole have been reported. C. krusei species is also reported as the only Candida sp. which has an intrinsic resistance factor to fluconazole. Therefore, in dealing with antifungal resistance, it is necessary to develop new antifungal agents that are efficient in the treatment of fungal infections, especially those caused by C. krusei. The purpose of this study was to investigate the genome of clinical C. krusei isolates and correlate the resistant phenotypes with mutations in resistance genes. A total of 16 samples of C. krusei from clinical samples from hospitals in Jakarta were used in the experiment. All colonies were extracted using the QIAamp DNA Mini Kit. The library was prepared using the Illumina DNA Prep Kit. The sequencing process was carried out on the Illumina MiSeq Platform using a 2x301 paired-end configuration. FASTQ raw files are available under the BioProject Accession Number PRJNA819536 and Sequence Read Archive Accession Numbers SRR18739949 and SRR18739964.https://f1000research.com/articles/11-593/v2Candida krusei fluconazole genomic profiling resistance geneeng |
| spellingShingle | Fadilah Fadilah Khaerunissa Anbar Istiadi Ayu Eka Fatril Linda Erlina Retno Wahyuningsih Anna Rozaliyani Rafika Indah Paramita Robiatul Adawiyah Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia [version 2; peer review: 2 approved] F1000Research Candida krusei fluconazole genomic profiling resistance gene eng |
| title | Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia [version 2; peer review: 2 approved] |
| title_full | Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia [version 2; peer review: 2 approved] |
| title_fullStr | Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia [version 2; peer review: 2 approved] |
| title_full_unstemmed | Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia [version 2; peer review: 2 approved] |
| title_short | Whole genome sequencing data of Candida krusei, the pathogen causing Candidaemia, from Department of Parasitology Culture Collection, Faculty of Medicine Universitas Indonesia [version 2; peer review: 2 approved] |
| title_sort | whole genome sequencing data of candida krusei the pathogen causing candidaemia from department of parasitology culture collection faculty of medicine universitas indonesia version 2 peer review 2 approved |
| topic | Candida krusei fluconazole genomic profiling resistance gene eng |
| url | https://f1000research.com/articles/11-593/v2 |
| work_keys_str_mv | AT fadilahfadilah wholegenomesequencingdataofcandidakruseithepathogencausingcandidaemiafromdepartmentofparasitologyculturecollectionfacultyofmedicineuniversitasindonesiaversion2peerreview2approved AT khaerunissaanbaristiadi wholegenomesequencingdataofcandidakruseithepathogencausingcandidaemiafromdepartmentofparasitologyculturecollectionfacultyofmedicineuniversitasindonesiaversion2peerreview2approved AT ayuekafatril wholegenomesequencingdataofcandidakruseithepathogencausingcandidaemiafromdepartmentofparasitologyculturecollectionfacultyofmedicineuniversitasindonesiaversion2peerreview2approved AT lindaerlina wholegenomesequencingdataofcandidakruseithepathogencausingcandidaemiafromdepartmentofparasitologyculturecollectionfacultyofmedicineuniversitasindonesiaversion2peerreview2approved AT retnowahyuningsih wholegenomesequencingdataofcandidakruseithepathogencausingcandidaemiafromdepartmentofparasitologyculturecollectionfacultyofmedicineuniversitasindonesiaversion2peerreview2approved AT annarozaliyani wholegenomesequencingdataofcandidakruseithepathogencausingcandidaemiafromdepartmentofparasitologyculturecollectionfacultyofmedicineuniversitasindonesiaversion2peerreview2approved AT rafikaindahparamita wholegenomesequencingdataofcandidakruseithepathogencausingcandidaemiafromdepartmentofparasitologyculturecollectionfacultyofmedicineuniversitasindonesiaversion2peerreview2approved AT robiatuladawiyah wholegenomesequencingdataofcandidakruseithepathogencausingcandidaemiafromdepartmentofparasitologyculturecollectionfacultyofmedicineuniversitasindonesiaversion2peerreview2approved |