Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria
<p>Abstract</p> <p>Background</p> <p>Plant endophytic bacteria play an important role benefiting plant growth or being pathogenic to plants or organisms that consume those plants. Multiple species of bacteria have been found co-inhabiting plants, both cultivated and wil...
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| Format: | Article |
| Language: | English |
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BMC
2013-01-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2180/13/1 |
| _version_ | 1819129584175546368 |
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| author | Ding Tao Palmer Michael W Melcher Ulrich |
| author_facet | Ding Tao Palmer Michael W Melcher Ulrich |
| author_sort | Ding Tao |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Plant endophytic bacteria play an important role benefiting plant growth or being pathogenic to plants or organisms that consume those plants. Multiple species of bacteria have been found co-inhabiting plants, both cultivated and wild, with viruses and fungi. For these reasons, a general understanding of plant endophytic microbial communities and their diversity is necessary. A key issue is how the distributions of these bacteria vary with location, with plant species, with individual plants and with plant growing season.</p> <p>Results</p> <p>Five common plant species were collected monthly for four months in the summer of 2010, with replicates from four different sampling sites in the Tallgrass Prairie Preserve in Osage County, Oklahoma, USA. Metagenomic DNA was extracted from ground, washed plant leaf samples, and fragments of the bacterial 16S rDNA genes were amplified for analysis of terminal restriction fragment length polymorphism (T-RFLP). We performed mono-digestion T-RFLP with restriction endonuclease <it>Dde</it>I, to reveal the structures of leaf endophytic bacterial communities, to identify the differences between plant-associated bacterial communities in different plant species or environments, and to explore factors affecting the bacterial distribution. We tested the impacts of three major factors on the leaf endophytic bacterial communities, including host plant species, sampling dates and sampling locations.</p> <p>Conclusions</p> <p>Results indicated that all of the three factors were significantly related (α = 0.05) to the distribution of leaf endophytic bacteria, with host species being the most important, followed by sampling dates and sampling locations.</p> |
| first_indexed | 2024-12-22T08:46:02Z |
| format | Article |
| id | doaj.art-f9fbf6ecdc3b4164aa6354f6756e2799 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-22T08:46:02Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-f9fbf6ecdc3b4164aa6354f6756e27992022-12-21T18:32:07ZengBMCBMC Microbiology1471-21802013-01-01131110.1186/1471-2180-13-1Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteriaDing TaoPalmer Michael WMelcher Ulrich<p>Abstract</p> <p>Background</p> <p>Plant endophytic bacteria play an important role benefiting plant growth or being pathogenic to plants or organisms that consume those plants. Multiple species of bacteria have been found co-inhabiting plants, both cultivated and wild, with viruses and fungi. For these reasons, a general understanding of plant endophytic microbial communities and their diversity is necessary. A key issue is how the distributions of these bacteria vary with location, with plant species, with individual plants and with plant growing season.</p> <p>Results</p> <p>Five common plant species were collected monthly for four months in the summer of 2010, with replicates from four different sampling sites in the Tallgrass Prairie Preserve in Osage County, Oklahoma, USA. Metagenomic DNA was extracted from ground, washed plant leaf samples, and fragments of the bacterial 16S rDNA genes were amplified for analysis of terminal restriction fragment length polymorphism (T-RFLP). We performed mono-digestion T-RFLP with restriction endonuclease <it>Dde</it>I, to reveal the structures of leaf endophytic bacterial communities, to identify the differences between plant-associated bacterial communities in different plant species or environments, and to explore factors affecting the bacterial distribution. We tested the impacts of three major factors on the leaf endophytic bacterial communities, including host plant species, sampling dates and sampling locations.</p> <p>Conclusions</p> <p>Results indicated that all of the three factors were significantly related (α = 0.05) to the distribution of leaf endophytic bacteria, with host species being the most important, followed by sampling dates and sampling locations.</p>http://www.biomedcentral.com/1471-2180/13/1Leaf bacterial endophytesEcologyT-RFLPBiodiversity |
| spellingShingle | Ding Tao Palmer Michael W Melcher Ulrich Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria BMC Microbiology Leaf bacterial endophytes Ecology T-RFLP Biodiversity |
| title | Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria |
| title_full | Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria |
| title_fullStr | Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria |
| title_full_unstemmed | Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria |
| title_short | Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria |
| title_sort | community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria |
| topic | Leaf bacterial endophytes Ecology T-RFLP Biodiversity |
| url | http://www.biomedcentral.com/1471-2180/13/1 |
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