Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing
Lactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera <i>Lactobacillus</i> and <i>Lactococcus</i>, little has been u...
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MDPI AG
2021-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/11/1520 |
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| author | Hiromasa Tanno Emi Kanno Shiho Kurosaka Yukari Oikawa Takumi Watanabe Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Shinyo Ishi Miki Shoji Naoyuki Takagi Yoshimichi Imai Keiko Ishii Masahiro Tachi Kazuyoshi Kawakami |
| author_facet | Hiromasa Tanno Emi Kanno Shiho Kurosaka Yukari Oikawa Takumi Watanabe Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Shinyo Ishi Miki Shoji Naoyuki Takagi Yoshimichi Imai Keiko Ishii Masahiro Tachi Kazuyoshi Kawakami |
| author_sort | Hiromasa Tanno |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera <i>Lactobacillus</i> and <i>Lactococcus</i>, little has been uncovered on the effects of the genus <i>Enterococcus</i> on skin wound-healing. In this study, we aimed to clarify the effect of heat-killed <i>Enterococcus faecalis</i> KH2 (heat-killed KH2) strain on the wound-healing process and to evaluate the therapeutic potential in chronic skin wounds. We analyzed percent wound closure, re-epithelialization, and granulation area, and cytokine and growth factor production. We found that heat-killed KH2 contributed to the acceleration of re-epithelialization and the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1, and vascular endothelial growth factor production. In addition, heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors. |
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| language | English |
| last_indexed | 2024-03-10T05:41:49Z |
| publishDate | 2021-10-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-f9fc564098254bf39ca3b5eabe7670e12023-11-22T22:29:26ZengMDPI AGBiomedicines2227-90592021-10-01911152010.3390/biomedicines9111520Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-HealingHiromasa Tanno0Emi Kanno1Shiho Kurosaka2Yukari Oikawa3Takumi Watanabe4Ko Sato5Jun Kasamatsu6Tomomitsu Miyasaka7Shinyo Ishi8Miki Shoji9Naoyuki Takagi10Yoshimichi Imai11Keiko Ishii12Masahiro Tachi13Kazuyoshi Kawakami14Department of Science of Nursing Practice, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Science of Nursing Practice, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanBio-Lab Co., Ltd., 2-1-3 Komagawa, Hidaka-shi 350-1249, JapanDepartment of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Intelligent Network for Infection Control, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDivision of Pathophysiology, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Sendai 981-8558, JapanDepartment of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanDepartment of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, JapanLactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera <i>Lactobacillus</i> and <i>Lactococcus</i>, little has been uncovered on the effects of the genus <i>Enterococcus</i> on skin wound-healing. In this study, we aimed to clarify the effect of heat-killed <i>Enterococcus faecalis</i> KH2 (heat-killed KH2) strain on the wound-healing process and to evaluate the therapeutic potential in chronic skin wounds. We analyzed percent wound closure, re-epithelialization, and granulation area, and cytokine and growth factor production. We found that heat-killed KH2 contributed to the acceleration of re-epithelialization and the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1, and vascular endothelial growth factor production. In addition, heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors.https://www.mdpi.com/2227-9059/9/11/1520wound-healing<i>Enterococcus faecalis</i> KH2angiogenesis |
| spellingShingle | Hiromasa Tanno Emi Kanno Shiho Kurosaka Yukari Oikawa Takumi Watanabe Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Shinyo Ishi Miki Shoji Naoyuki Takagi Yoshimichi Imai Keiko Ishii Masahiro Tachi Kazuyoshi Kawakami Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing Biomedicines wound-healing <i>Enterococcus faecalis</i> KH2 angiogenesis |
| title | Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing |
| title_full | Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing |
| title_fullStr | Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing |
| title_full_unstemmed | Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing |
| title_short | Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing |
| title_sort | topical administration of heat killed i enterococcus faecalis i strain kh2 promotes re epithelialization and granulation tissue formation during skin wound healing |
| topic | wound-healing <i>Enterococcus faecalis</i> KH2 angiogenesis |
| url | https://www.mdpi.com/2227-9059/9/11/1520 |
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