Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes
ABSTRACTIn December 2019, a novel coronavirus (2019-nCoV) caused an outbreak in Wuhan, China, and soon spread to other parts of the world. It was believed that 2019-nCoV was transmitted through respiratory tract and then induced pneumonia, thus molecular diagnosis based on oral swabs was used for co...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1729071 |
| _version_ | 1797265910360178688 |
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| author | Wei Zhang Rong-Hui Du Bei Li Xiao-Shuang Zheng Xing-Lou Yang Ben Hu Yan-Yi Wang Geng-Fu Xiao Bing Yan Zheng-Li Shi Peng Zhou |
| author_facet | Wei Zhang Rong-Hui Du Bei Li Xiao-Shuang Zheng Xing-Lou Yang Ben Hu Yan-Yi Wang Geng-Fu Xiao Bing Yan Zheng-Li Shi Peng Zhou |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | ABSTRACTIn December 2019, a novel coronavirus (2019-nCoV) caused an outbreak in Wuhan, China, and soon spread to other parts of the world. It was believed that 2019-nCoV was transmitted through respiratory tract and then induced pneumonia, thus molecular diagnosis based on oral swabs was used for confirmation of this disease. Likewise, patient will be released upon two times of negative detection from oral swabs. However, many coronaviruses can also be transmitted through oral–fecal route by infecting intestines. Whether 2019-nCoV infected patients also carry virus in other organs like intestine need to be tested. We conducted investigation on patients in a local hospital who were infected with this virus. We found the presence of 2019-nCoV in anal swabs and blood as well, and more anal swab positives than oral swab positives in a later stage of infection, suggesting shedding and thereby transmitted through oral–fecal route. We also showed serology test can improve detection positive rate thus should be used in future epidemiology. Our report provides a cautionary warning that 2019-nCoV may be shed through multiple routes. |
| first_indexed | 2024-03-07T17:24:41Z |
| format | Article |
| id | doaj.art-f9fd26854b7c4bd4b2a427be77a1808e |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:52:18Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-f9fd26854b7c4bd4b2a427be77a1808e2024-03-11T16:04:23ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019138638910.1080/22221751.2020.1729071Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routesWei Zhang0Rong-Hui Du1Bei Li2Xiao-Shuang Zheng3Xing-Lou Yang4Ben Hu5Yan-Yi Wang6Geng-Fu Xiao7Bing Yan8Zheng-Li Shi9Peng Zhou10CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaWuhan Pulmonary Hospital, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People’s Republic of ChinaABSTRACTIn December 2019, a novel coronavirus (2019-nCoV) caused an outbreak in Wuhan, China, and soon spread to other parts of the world. It was believed that 2019-nCoV was transmitted through respiratory tract and then induced pneumonia, thus molecular diagnosis based on oral swabs was used for confirmation of this disease. Likewise, patient will be released upon two times of negative detection from oral swabs. However, many coronaviruses can also be transmitted through oral–fecal route by infecting intestines. Whether 2019-nCoV infected patients also carry virus in other organs like intestine need to be tested. We conducted investigation on patients in a local hospital who were infected with this virus. We found the presence of 2019-nCoV in anal swabs and blood as well, and more anal swab positives than oral swab positives in a later stage of infection, suggesting shedding and thereby transmitted through oral–fecal route. We also showed serology test can improve detection positive rate thus should be used in future epidemiology. Our report provides a cautionary warning that 2019-nCoV may be shed through multiple routes.https://www.tandfonline.com/doi/10.1080/22221751.2020.17290712019-nCoVWuhan pneumoniaepidemiologyswabsintestine |
| spellingShingle | Wei Zhang Rong-Hui Du Bei Li Xiao-Shuang Zheng Xing-Lou Yang Ben Hu Yan-Yi Wang Geng-Fu Xiao Bing Yan Zheng-Li Shi Peng Zhou Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes Emerging Microbes and Infections 2019-nCoV Wuhan pneumonia epidemiology swabs intestine |
| title | Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes |
| title_full | Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes |
| title_fullStr | Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes |
| title_full_unstemmed | Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes |
| title_short | Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes |
| title_sort | molecular and serological investigation of 2019 ncov infected patients implication of multiple shedding routes |
| topic | 2019-nCoV Wuhan pneumonia epidemiology swabs intestine |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1729071 |
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