Use of Callistemon citrinus as a gastroprotective and anti-inflammatory agent on indomethacin-induced gastric ulcers in obese rats
Background Obesity leads to an elevated risk of developing gastrointestinal disease such as gastric ulcers. Callistemon citrinus leaf extract has shown antioxidant, antimicrobial, hepatoprotective, and chemoprotective effects against colon cancer. The aim of this study is to evaluate the gastroprote...
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PeerJ Inc.
2024-02-01
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| author | Jonathan Saúl Piñón-Simental Luis Alberto Ayala-Ruiz Luis Gerardo Ortega-Pérez Oliver Rafid Magaña-Rodríguez Esperanza Meléndez-Herrera Asdrubal Aguilera-Méndez Patricia Rios-Chavez |
| author_facet | Jonathan Saúl Piñón-Simental Luis Alberto Ayala-Ruiz Luis Gerardo Ortega-Pérez Oliver Rafid Magaña-Rodríguez Esperanza Meléndez-Herrera Asdrubal Aguilera-Méndez Patricia Rios-Chavez |
| author_sort | Jonathan Saúl Piñón-Simental |
| collection | DOAJ |
| description | Background Obesity leads to an elevated risk of developing gastrointestinal disease such as gastric ulcers. Callistemon citrinus leaf extract has shown antioxidant, antimicrobial, hepatoprotective, and chemoprotective effects against colon cancer. The aim of this study is to evaluate the gastroprotective effect of C. citrinus leaf extract on indomethacin-induced gastric ulcers in obese rats. Methods Gastric ulcers were induced in female obese Wistar rats using a single oral dose of indomethacin (IND). In the first stage, the rats were fed with a high fat sugar diet (HFSD) for 15 weeks to induce obesity and, at the same time, the diet of the other group of animals included daily administration of ethanolic C. citrinus leaf extract (250 mg/kg) in addition to HFSD. In the second stage, gastric ulcers were induced with IND (30 mg/kg). The gastroprotective activity of C. citrinus, the inflammatory enzyme activities, and cytokines in the stomach were determined. Results C. citrinus produced a reduction of gastric lesions caused by IND. Myeloperoxidase (MPO), cyclooxygenase-2 (COX-2), and 5-lipoxygenase (5-LOX) activities also decreased. Although inflammatory biomarkers such as TNFα, IL-6, AOPP, and leptin were significantly decreased by C. citrinus, adiponectin levels increased. Moreover, C. citrinus decreased weight gain and morphological and biochemical parameters. Conclusion The use of indomethacin in rats fed with a high fat-sugar diet increased gastric ulcers. Gastroprotective effect of C. citrinus in obese rats is attributed to the reduction of pro-inflammatory cytokines and the inflammatory enzymes. |
| first_indexed | 2024-03-07T19:00:56Z |
| format | Article |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-07T19:00:56Z |
| publishDate | 2024-02-01 |
| publisher | PeerJ Inc. |
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| series | PeerJ |
| spelling | doaj.art-f9ff624b0abd427ab5810570d986897e2024-03-01T15:05:35ZengPeerJ Inc.PeerJ2167-83592024-02-0112e1706210.7717/peerj.17062Use of Callistemon citrinus as a gastroprotective and anti-inflammatory agent on indomethacin-induced gastric ulcers in obese ratsJonathan Saúl Piñón-Simental0Luis Alberto Ayala-Ruiz1Luis Gerardo Ortega-Pérez2Oliver Rafid Magaña-Rodríguez3Esperanza Meléndez-Herrera4Asdrubal Aguilera-Méndez5Patricia Rios-Chavez6Biologia, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacan, MexicoBiologia, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacan, MexicoBiologia, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacan, MexicoBiologia, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacan, MexicoInstituto de Investigaciones sobre los Recursos Naturales, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacan, MexicoInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacan, MexicoBiologia, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacan, MexicoBackground Obesity leads to an elevated risk of developing gastrointestinal disease such as gastric ulcers. Callistemon citrinus leaf extract has shown antioxidant, antimicrobial, hepatoprotective, and chemoprotective effects against colon cancer. The aim of this study is to evaluate the gastroprotective effect of C. citrinus leaf extract on indomethacin-induced gastric ulcers in obese rats. Methods Gastric ulcers were induced in female obese Wistar rats using a single oral dose of indomethacin (IND). In the first stage, the rats were fed with a high fat sugar diet (HFSD) for 15 weeks to induce obesity and, at the same time, the diet of the other group of animals included daily administration of ethanolic C. citrinus leaf extract (250 mg/kg) in addition to HFSD. In the second stage, gastric ulcers were induced with IND (30 mg/kg). The gastroprotective activity of C. citrinus, the inflammatory enzyme activities, and cytokines in the stomach were determined. Results C. citrinus produced a reduction of gastric lesions caused by IND. Myeloperoxidase (MPO), cyclooxygenase-2 (COX-2), and 5-lipoxygenase (5-LOX) activities also decreased. Although inflammatory biomarkers such as TNFα, IL-6, AOPP, and leptin were significantly decreased by C. citrinus, adiponectin levels increased. Moreover, C. citrinus decreased weight gain and morphological and biochemical parameters. Conclusion The use of indomethacin in rats fed with a high fat-sugar diet increased gastric ulcers. Gastroprotective effect of C. citrinus in obese rats is attributed to the reduction of pro-inflammatory cytokines and the inflammatory enzymes.https://peerj.com/articles/17062.pdfGastric ulcersInflammatoryCallistemon citrinusBiomarkersIndomethacinObesity |
| spellingShingle | Jonathan Saúl Piñón-Simental Luis Alberto Ayala-Ruiz Luis Gerardo Ortega-Pérez Oliver Rafid Magaña-Rodríguez Esperanza Meléndez-Herrera Asdrubal Aguilera-Méndez Patricia Rios-Chavez Use of Callistemon citrinus as a gastroprotective and anti-inflammatory agent on indomethacin-induced gastric ulcers in obese rats PeerJ Gastric ulcers Inflammatory Callistemon citrinus Biomarkers Indomethacin Obesity |
| title | Use of Callistemon citrinus as a gastroprotective and anti-inflammatory agent on indomethacin-induced gastric ulcers in obese rats |
| title_full | Use of Callistemon citrinus as a gastroprotective and anti-inflammatory agent on indomethacin-induced gastric ulcers in obese rats |
| title_fullStr | Use of Callistemon citrinus as a gastroprotective and anti-inflammatory agent on indomethacin-induced gastric ulcers in obese rats |
| title_full_unstemmed | Use of Callistemon citrinus as a gastroprotective and anti-inflammatory agent on indomethacin-induced gastric ulcers in obese rats |
| title_short | Use of Callistemon citrinus as a gastroprotective and anti-inflammatory agent on indomethacin-induced gastric ulcers in obese rats |
| title_sort | use of callistemon citrinus as a gastroprotective and anti inflammatory agent on indomethacin induced gastric ulcers in obese rats |
| topic | Gastric ulcers Inflammatory Callistemon citrinus Biomarkers Indomethacin Obesity |
| url | https://peerj.com/articles/17062.pdf |
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