Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies
Pulsed electric fields (PEFs) have become clinically important through the success of Irreversible Electroporation (IRE), Electrochemotherapy (ECT), and nanosecond PEFs (nsPEFs) for the treatment of tumors. PEFs increase the permeability of cell membranes, a phenomenon known as electroporation. In a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/12/5/1132 |
| _version_ | 1797569164737511424 |
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| author | Philip M. Graybill Rafael V. Davalos |
| author_facet | Philip M. Graybill Rafael V. Davalos |
| author_sort | Philip M. Graybill |
| collection | DOAJ |
| description | Pulsed electric fields (PEFs) have become clinically important through the success of Irreversible Electroporation (IRE), Electrochemotherapy (ECT), and nanosecond PEFs (nsPEFs) for the treatment of tumors. PEFs increase the permeability of cell membranes, a phenomenon known as electroporation. In addition to well-known membrane effects, PEFs can cause profound cytoskeletal disruption. In this review, we summarize the current understanding of cytoskeletal disruption after PEFs. Compiling available studies, we describe PEF-induced cytoskeletal disruption and possible mechanisms of disruption. Additionally, we consider how cytoskeletal alterations contribute to cell–cell and cell–substrate disruption. We conclude with a discussion of cytoskeletal disruption-induced anti-vascular effects of PEFs and consider how a better understanding of cytoskeletal disruption after PEFs may lead to more effective therapies. |
| first_indexed | 2024-03-10T20:08:01Z |
| format | Article |
| id | doaj.art-f9ffee2293bb4af985891343af8ee6ec |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T20:08:01Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-f9ffee2293bb4af985891343af8ee6ec2023-11-19T23:11:57ZengMDPI AGCancers2072-66942020-04-01125113210.3390/cancers12051132Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field TherapiesPhilip M. Graybill0Rafael V. Davalos1BEMS Lab, Department of Biomedical Engineering and Mechanics, Virginia Tech, Blacksburg, VA 24061, USABEMS Lab, Department of Biomedical Engineering and Mechanics, Virginia Tech, Blacksburg, VA 24061, USAPulsed electric fields (PEFs) have become clinically important through the success of Irreversible Electroporation (IRE), Electrochemotherapy (ECT), and nanosecond PEFs (nsPEFs) for the treatment of tumors. PEFs increase the permeability of cell membranes, a phenomenon known as electroporation. In addition to well-known membrane effects, PEFs can cause profound cytoskeletal disruption. In this review, we summarize the current understanding of cytoskeletal disruption after PEFs. Compiling available studies, we describe PEF-induced cytoskeletal disruption and possible mechanisms of disruption. Additionally, we consider how cytoskeletal alterations contribute to cell–cell and cell–substrate disruption. We conclude with a discussion of cytoskeletal disruption-induced anti-vascular effects of PEFs and consider how a better understanding of cytoskeletal disruption after PEFs may lead to more effective therapies.https://www.mdpi.com/2072-6694/12/5/1132pulsed electric fieldselectroporationcytoskeletonactinmicrotubulesintermediate filaments |
| spellingShingle | Philip M. Graybill Rafael V. Davalos Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies Cancers pulsed electric fields electroporation cytoskeleton actin microtubules intermediate filaments |
| title | Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies |
| title_full | Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies |
| title_fullStr | Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies |
| title_full_unstemmed | Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies |
| title_short | Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies |
| title_sort | cytoskeletal disruption after electroporation and its significance to pulsed electric field therapies |
| topic | pulsed electric fields electroporation cytoskeleton actin microtubules intermediate filaments |
| url | https://www.mdpi.com/2072-6694/12/5/1132 |
| work_keys_str_mv | AT philipmgraybill cytoskeletaldisruptionafterelectroporationanditssignificancetopulsedelectricfieldtherapies AT rafaelvdavalos cytoskeletaldisruptionafterelectroporationanditssignificancetopulsedelectricfieldtherapies |