Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells
Introduction:A. truncatum Bunge (Sapindaceae or formerly Aceraceae) is a tall deciduous tree native to China. Traditionally, the leaves of A. truncatum are decocted and used by Chinese Mongolians, Koreans, and Tibetans to treat skin itching, dry cracks, and other skin ailments, which indicates A. tr...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1068849/full |
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| author | Yanxiao Fan Yanxiao Fan Ronghui Gu Ronghui Gu Ruifei Zhang Ruifei Zhang Miaomiao Wang Miaomiao Wang Heran Xu Heran Xu Min Wang Min Wang Chunlin Long Chunlin Long Chunlin Long Chunlin Long |
| author_facet | Yanxiao Fan Yanxiao Fan Ronghui Gu Ronghui Gu Ruifei Zhang Ruifei Zhang Miaomiao Wang Miaomiao Wang Heran Xu Heran Xu Min Wang Min Wang Chunlin Long Chunlin Long Chunlin Long Chunlin Long |
| author_sort | Yanxiao Fan |
| collection | DOAJ |
| description | Introduction:A. truncatum Bunge (Sapindaceae or formerly Aceraceae) is a tall deciduous tree native to China. Traditionally, the leaves of A. truncatum are decocted and used by Chinese Mongolians, Koreans, and Tibetans to treat skin itching, dry cracks, and other skin ailments, which indicates A. truncatum leaves may have a potential inhibitory effect on various skin inflammations.Methods: To examine the protective effect against skin inflammations of A. truncatum leaf extract (ATLE), an in vitro dermatitis model was established using sodium dodecyl sulfate (SLS)-induced HaCaT cells. The anti-inflammatory effect of ATLE was evaluated by analyzing cell viability, apoptosis, reactive oxygen species (ROS), interleukin 6 (IL-6), and prostaglandin E2 (PGE2) levels.Results: Orthogonal experiments showed that the pretreatment with ATLE can reduce the IL-6 levels, PGE2 levels, and apoptosis increased in SLS-stimulated HaCaT cells, which indicates that ATLE has positive efficacy for dermatitis. Furthermore, three flavonoid compounds kaempferol-3-O-α-L-rhamnoside, quercetin-3-O-α-L-rhamnopyranoside, kaempferol-3,7-di-O-α-L-rhamnoside, and 1,2,3,4,6-Penta-O-galloyl-β-D-glucopyranose (PGG) were isolated and identified. Among them, kaempferol-3,7-di-O-α-L-rhamnoside was isolated from this plant for the first time. These compounds have been proven to have an anti-inflammatory effect. They may contribute to the efficacy of A. truncatumin treating skin inflammation.Discussion: The results revealed that ATLE has the potential to be used as an additive in various skin care products to prevent skin inflammations and may be incorporated in formulations for topical application as a therapeutic approach against dermatitis. |
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| id | doaj.art-fa025c38300748ed8c2ed400789e7aba |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-10T00:31:35Z |
| publishDate | 2023-03-01 |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-fa025c38300748ed8c2ed400789e7aba2023-03-15T04:24:03ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-03-011410.3389/fphar.2023.10688491068849Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cellsYanxiao Fan0Yanxiao Fan1Ronghui Gu2Ronghui Gu3Ruifei Zhang4Ruifei Zhang5Miaomiao Wang6Miaomiao Wang7Heran Xu8Heran Xu9Min Wang10Min Wang11Chunlin Long12Chunlin Long13Chunlin Long14Chunlin Long15Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Guizhou University), Ministry of Education, Guiyang, ChinaSchool of Liquor and Food Engineering, Guizhou University, Guiyang, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaCollege of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing, ChinaBTBU-TANGYI Innovation Center for the Evaluation of the Safety and Efficacy of Bioengineering Raw Materials, Beijing, ChinaCollege of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing, ChinaBTBU-TANGYI Innovation Center for the Evaluation of the Safety and Efficacy of Bioengineering Raw Materials, Beijing, ChinaKey Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing, ChinaKey Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing, ChinaInstitute of National Security Studies, Minzu University of China, Beijing, ChinaIntroduction:A. truncatum Bunge (Sapindaceae or formerly Aceraceae) is a tall deciduous tree native to China. Traditionally, the leaves of A. truncatum are decocted and used by Chinese Mongolians, Koreans, and Tibetans to treat skin itching, dry cracks, and other skin ailments, which indicates A. truncatum leaves may have a potential inhibitory effect on various skin inflammations.Methods: To examine the protective effect against skin inflammations of A. truncatum leaf extract (ATLE), an in vitro dermatitis model was established using sodium dodecyl sulfate (SLS)-induced HaCaT cells. The anti-inflammatory effect of ATLE was evaluated by analyzing cell viability, apoptosis, reactive oxygen species (ROS), interleukin 6 (IL-6), and prostaglandin E2 (PGE2) levels.Results: Orthogonal experiments showed that the pretreatment with ATLE can reduce the IL-6 levels, PGE2 levels, and apoptosis increased in SLS-stimulated HaCaT cells, which indicates that ATLE has positive efficacy for dermatitis. Furthermore, three flavonoid compounds kaempferol-3-O-α-L-rhamnoside, quercetin-3-O-α-L-rhamnopyranoside, kaempferol-3,7-di-O-α-L-rhamnoside, and 1,2,3,4,6-Penta-O-galloyl-β-D-glucopyranose (PGG) were isolated and identified. Among them, kaempferol-3,7-di-O-α-L-rhamnoside was isolated from this plant for the first time. These compounds have been proven to have an anti-inflammatory effect. They may contribute to the efficacy of A. truncatumin treating skin inflammation.Discussion: The results revealed that ATLE has the potential to be used as an additive in various skin care products to prevent skin inflammations and may be incorporated in formulations for topical application as a therapeutic approach against dermatitis.https://www.frontiersin.org/articles/10.3389/fphar.2023.1068849/fullA. truncatumdermatitisinterleukin 6flavonoidsskin inflammations |
| spellingShingle | Yanxiao Fan Yanxiao Fan Ronghui Gu Ronghui Gu Ruifei Zhang Ruifei Zhang Miaomiao Wang Miaomiao Wang Heran Xu Heran Xu Min Wang Min Wang Chunlin Long Chunlin Long Chunlin Long Chunlin Long Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells Frontiers in Pharmacology A. truncatum dermatitis interleukin 6 flavonoids skin inflammations |
| title | Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells |
| title_full | Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells |
| title_fullStr | Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells |
| title_full_unstemmed | Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells |
| title_short | Protective effects of extracts from Acer truncatum leaves on SLS-induced HaCaT cells |
| title_sort | protective effects of extracts from acer truncatum leaves on sls induced hacat cells |
| topic | A. truncatum dermatitis interleukin 6 flavonoids skin inflammations |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1068849/full |
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