Immunohistochemical and Electron Microscopic Study of the Inhibitory Effects of Olive Oil Polyphenol on Dexamethasone-Induced Apoptosis
Background: There is accumulating evidence that a polyphenol present in olive oil, oleuropein, has antioxidant, anti-inflammatory and anti-apoptotic effects. This study aimed at determining the anti-apoptotic effect of Oleuropein (Ole) on dexamethasone-induced apoptosis of mouse thymocytes. Method: ...
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| Format: | Article |
| Language: | English |
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Iranian Society of Pathology
2017-01-01
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| Series: | Iranian Journal of Pathology |
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| Online Access: | https://ijp.iranpath.org/article_24227_7df9a1f20a584fcc74b226afdcd9ac0b.pdf |
| _version_ | 1819008916472725504 |
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| author | Ali Reza Khalatbary Behrooz Mohammadnegad Ghazaleh Goudarzi Ali Fazlollahpour Balef |
| author_facet | Ali Reza Khalatbary Behrooz Mohammadnegad Ghazaleh Goudarzi Ali Fazlollahpour Balef |
| author_sort | Ali Reza Khalatbary |
| collection | DOAJ |
| description | Background: There is accumulating evidence that a polyphenol present in olive oil, oleuropein, has antioxidant, anti-inflammatory and anti-apoptotic effects. This study aimed at determining the anti-apoptotic effect of Oleuropein (Ole) on dexamethasone-induced apoptosis of mouse thymocytes. Method: Mice were randomly divided to four groups as follow: Dexamethasone (Dex)-treated group (20 mg/kg; single dose), Ole-treated group (20 mg/kg per day), Dex plus Ole-treated group, and vehicle group. Sections of thymus were taken 16 hours after dexamethasone injection and studied for histopathological and immunohistochemistry assessment. Result: Further characteristics of degeneration in thymocytes were observed in the Dex group compared with the Dex plus Ole group. Compared with the Dex group (10.94±3.35), positive staining for Bax in thymocytes decreased in Dex plus Ole group (2.64±1.26), but remained higher than the Ole (0.65±0.30) and vehicle (0.67±0.29) groups. Compared with the Dex group (2.94±0.42), positive staining for Bcl-2 in thymocytes increased in Dex plus Ole group (12.24±1.84) yet was lower than the Ole (14.94±1.54) and vehicle (18.93±3.54) groups. Conclusion: Our results suggest that dexamethasone-induced apoptosis is subsided by oleuropein. |
| first_indexed | 2024-12-21T00:48:04Z |
| format | Article |
| id | doaj.art-fa045c34d7a54bc8bed94ef4775d4f3f |
| institution | Directory Open Access Journal |
| issn | 1735-5303 2345-3656 |
| language | English |
| last_indexed | 2024-12-21T00:48:04Z |
| publishDate | 2017-01-01 |
| publisher | Iranian Society of Pathology |
| record_format | Article |
| series | Iranian Journal of Pathology |
| spelling | doaj.art-fa045c34d7a54bc8bed94ef4775d4f3f2022-12-21T19:21:28ZengIranian Society of PathologyIranian Journal of Pathology1735-53032345-36562017-01-01121455210.30699/ijp.2017.2422724227Immunohistochemical and Electron Microscopic Study of the Inhibitory Effects of Olive Oil Polyphenol on Dexamethasone-Induced ApoptosisAli Reza Khalatbary0Behrooz Mohammadnegad1Ghazaleh Goudarzi2Ali Fazlollahpour Balef3Dept. of Anatomy, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranDept. of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranDept. of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranDept. of microbiology, Islamic Azad University, Qaemshar, IranBackground: There is accumulating evidence that a polyphenol present in olive oil, oleuropein, has antioxidant, anti-inflammatory and anti-apoptotic effects. This study aimed at determining the anti-apoptotic effect of Oleuropein (Ole) on dexamethasone-induced apoptosis of mouse thymocytes. Method: Mice were randomly divided to four groups as follow: Dexamethasone (Dex)-treated group (20 mg/kg; single dose), Ole-treated group (20 mg/kg per day), Dex plus Ole-treated group, and vehicle group. Sections of thymus were taken 16 hours after dexamethasone injection and studied for histopathological and immunohistochemistry assessment. Result: Further characteristics of degeneration in thymocytes were observed in the Dex group compared with the Dex plus Ole group. Compared with the Dex group (10.94±3.35), positive staining for Bax in thymocytes decreased in Dex plus Ole group (2.64±1.26), but remained higher than the Ole (0.65±0.30) and vehicle (0.67±0.29) groups. Compared with the Dex group (2.94±0.42), positive staining for Bcl-2 in thymocytes increased in Dex plus Ole group (12.24±1.84) yet was lower than the Ole (14.94±1.54) and vehicle (18.93±3.54) groups. Conclusion: Our results suggest that dexamethasone-induced apoptosis is subsided by oleuropein.https://ijp.iranpath.org/article_24227_7df9a1f20a584fcc74b226afdcd9ac0b.pdfdexamethasonethymocyteapoptosisoleuropein |
| spellingShingle | Ali Reza Khalatbary Behrooz Mohammadnegad Ghazaleh Goudarzi Ali Fazlollahpour Balef Immunohistochemical and Electron Microscopic Study of the Inhibitory Effects of Olive Oil Polyphenol on Dexamethasone-Induced Apoptosis Iranian Journal of Pathology dexamethasone thymocyte apoptosis oleuropein |
| title | Immunohistochemical and Electron Microscopic Study of the Inhibitory Effects of Olive Oil Polyphenol on Dexamethasone-Induced Apoptosis |
| title_full | Immunohistochemical and Electron Microscopic Study of the Inhibitory Effects of Olive Oil Polyphenol on Dexamethasone-Induced Apoptosis |
| title_fullStr | Immunohistochemical and Electron Microscopic Study of the Inhibitory Effects of Olive Oil Polyphenol on Dexamethasone-Induced Apoptosis |
| title_full_unstemmed | Immunohistochemical and Electron Microscopic Study of the Inhibitory Effects of Olive Oil Polyphenol on Dexamethasone-Induced Apoptosis |
| title_short | Immunohistochemical and Electron Microscopic Study of the Inhibitory Effects of Olive Oil Polyphenol on Dexamethasone-Induced Apoptosis |
| title_sort | immunohistochemical and electron microscopic study of the inhibitory effects of olive oil polyphenol on dexamethasone induced apoptosis |
| topic | dexamethasone thymocyte apoptosis oleuropein |
| url | https://ijp.iranpath.org/article_24227_7df9a1f20a584fcc74b226afdcd9ac0b.pdf |
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