A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication

A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication

The unavailability of tractable reverse genetic analysis approaches represents an obstacle to a better understanding of mitochondrial DNA replication. Here, we used CRISPR-Cas9 mediated gene editing to establish the conditional viability of knockouts in the key proteins involved in mtDNA replication...

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Bibliographic Details
Main Authors: Natalya Kozhukhar, Domenico Spadafora, Yelitza A. R. Rodriguez, Mikhail F. Alexeyev
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Cells
Subjects:
GeneSwap approach
mtDNA replication
mtDNA metabolism
mtDNA transcription
mtDNA instability
TFAM
Online Access:https://www.mdpi.com/2073-4409/11/14/2168

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