Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models
Syzygium aqueum is widely used in folk medicine. A polyphenol-rich extract from its leaves demonstrated a plethora of substantial pharmacological properties. The extract showed solid antioxidant properties in vitro and protected human keratinocytes (HaCaT cells) against UVA damage. The extract also...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.00566/full |
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| author | Mansour Sobeh Mona F. Mahmoud Ganna Petruk Samar Rezq Mohamed L. Ashour Fadia S. Youssef Assem M. El-Shazly Daria M. Monti Ashraf B. Abdel-Naim Michael Wink |
| author_facet | Mansour Sobeh Mona F. Mahmoud Ganna Petruk Samar Rezq Mohamed L. Ashour Fadia S. Youssef Assem M. El-Shazly Daria M. Monti Ashraf B. Abdel-Naim Michael Wink |
| author_sort | Mansour Sobeh |
| collection | DOAJ |
| description | Syzygium aqueum is widely used in folk medicine. A polyphenol-rich extract from its leaves demonstrated a plethora of substantial pharmacological properties. The extract showed solid antioxidant properties in vitro and protected human keratinocytes (HaCaT cells) against UVA damage. The extract also reduced the elevated levels of ALT, AST, total bilirubin (TB), total cholesterol (TC) and triglycerides (TG) in rats with acute CCl4 intoxication. In addition to reducing the high MDA level, the extract noticeably restored GSH and SOD to the normal control levels in liver tissue homogenates and counteracted the deleterious histopathologic changes in liver after CCl4 injection. Additionally, the extract exhibited promising anti-inflammatory activities in vitro where it inhibited LOX, COX-1, and COX-2 with a higher COX-2 selectivity than that of indomethacin and diclofenac and reduced the extent of lysis of erythrocytes upon incubation with hypotonic buffer solution. S. aqueum extract also markedly reduced leukocyte numbers with similar activities to diclofenac in rats challenged with carrageenan. Additionally, administration of the extract abolished writhes induced by acetic acid in mice and prolonged the response latency in hot plate test. Meanwhile, the identified polyphenolics from the extract showed a certain affinity for the active pockets of 5-lipoxygenase (5-LOX), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) explaining the observed anti-inflammatory activities. Finally, 87 secondary metabolites (mostly phenolics) were tentatively identified in the extract based on LC-MS/MS analyses. Syzygium aqueum displays good protection against oxidative stress, free radicals, and could be a good candidate for treating oxidative stress related diseases. |
| first_indexed | 2024-12-14T12:44:47Z |
| format | Article |
| id | doaj.art-fa06ad42d9ec448da9535de8211a0c47 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-14T12:44:47Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-fa06ad42d9ec448da9535de8211a0c472022-12-21T23:00:49ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-06-01910.3389/fphar.2018.00566376549Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal ModelsMansour Sobeh0Mona F. Mahmoud1Ganna Petruk2Samar Rezq3Mohamed L. Ashour4Fadia S. Youssef5Assem M. El-Shazly6Daria M. Monti7Ashraf B. Abdel-Naim8Michael Wink9Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, GermanyDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig, EgyptDepartment of Chemical Sciences, University of Naples Federico II, Naples, ItalyDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain Shams University Abbassia, Cairo, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain Shams University Abbassia, Cairo, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, EgyptDepartment of Chemical Sciences, University of Naples Federico II, Naples, ItalyDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi ArabiaInstitute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, GermanySyzygium aqueum is widely used in folk medicine. A polyphenol-rich extract from its leaves demonstrated a plethora of substantial pharmacological properties. The extract showed solid antioxidant properties in vitro and protected human keratinocytes (HaCaT cells) against UVA damage. The extract also reduced the elevated levels of ALT, AST, total bilirubin (TB), total cholesterol (TC) and triglycerides (TG) in rats with acute CCl4 intoxication. In addition to reducing the high MDA level, the extract noticeably restored GSH and SOD to the normal control levels in liver tissue homogenates and counteracted the deleterious histopathologic changes in liver after CCl4 injection. Additionally, the extract exhibited promising anti-inflammatory activities in vitro where it inhibited LOX, COX-1, and COX-2 with a higher COX-2 selectivity than that of indomethacin and diclofenac and reduced the extent of lysis of erythrocytes upon incubation with hypotonic buffer solution. S. aqueum extract also markedly reduced leukocyte numbers with similar activities to diclofenac in rats challenged with carrageenan. Additionally, administration of the extract abolished writhes induced by acetic acid in mice and prolonged the response latency in hot plate test. Meanwhile, the identified polyphenolics from the extract showed a certain affinity for the active pockets of 5-lipoxygenase (5-LOX), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) explaining the observed anti-inflammatory activities. Finally, 87 secondary metabolites (mostly phenolics) were tentatively identified in the extract based on LC-MS/MS analyses. Syzygium aqueum displays good protection against oxidative stress, free radicals, and could be a good candidate for treating oxidative stress related diseases.https://www.frontiersin.org/article/10.3389/fphar.2018.00566/fullSyzygium aqueumpolyphenolsantioxidanthepatoprotectivepain-killinganti-inflammatory |
| spellingShingle | Mansour Sobeh Mona F. Mahmoud Ganna Petruk Samar Rezq Mohamed L. Ashour Fadia S. Youssef Assem M. El-Shazly Daria M. Monti Ashraf B. Abdel-Naim Michael Wink Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models Frontiers in Pharmacology Syzygium aqueum polyphenols antioxidant hepatoprotective pain-killing anti-inflammatory |
| title | Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models |
| title_full | Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models |
| title_fullStr | Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models |
| title_full_unstemmed | Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models |
| title_short | Syzygium aqueum: A Polyphenol- Rich Leaf Extract Exhibits Antioxidant, Hepatoprotective, Pain-Killing and Anti-inflammatory Activities in Animal Models |
| title_sort | syzygium aqueum a polyphenol rich leaf extract exhibits antioxidant hepatoprotective pain killing and anti inflammatory activities in animal models |
| topic | Syzygium aqueum polyphenols antioxidant hepatoprotective pain-killing anti-inflammatory |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.00566/full |
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