Saponins from <i>Camellia sinensis</i> Seeds Stimulate GIP Secretion in Mice and STC-1 Cells via SGLT1 and TGR5
Glucose-dependent insulinotropic polypeptide (GIP) is one of the important incretins and possesses lots of physiological activities such as stimulating insulin secretion and maintaining glucose homeostasis. The pentacyclic triterpenoid saponins are the major active ingredients in tea (<i>Camel...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/14/16/3413 |
| _version_ | 1797431866247086080 |
|---|---|
| author | Huanqing Zhu Kaixi Wang Shuna Chen Jiaxin Kang Na Guo Hongbo Chen Junsheng Liu Yuanyuan Wu Puming He Youying Tu Bo Li |
| author_facet | Huanqing Zhu Kaixi Wang Shuna Chen Jiaxin Kang Na Guo Hongbo Chen Junsheng Liu Yuanyuan Wu Puming He Youying Tu Bo Li |
| author_sort | Huanqing Zhu |
| collection | DOAJ |
| description | Glucose-dependent insulinotropic polypeptide (GIP) is one of the important incretins and possesses lots of physiological activities such as stimulating insulin secretion and maintaining glucose homeostasis. The pentacyclic triterpenoid saponins are the major active ingredients in tea (<i>Camellia sinensis</i>) seeds. This study aimed to investigate the effect of tea seed saponins on the GIP secretion and related mechanisms. Our data showed that the total tea seed saponins (TSS, 65 mg/kg BW) and theasaponin E<sub>1</sub> (TSE1, 2–4 µM) could increase the GIP mRNA and protein levels in mice and STC-1 cells. Phlorizin, the inhibitor of Sodium/glucose cotransporter 1 (SGLT1), reversed the TSE1-induced increase in Ca<sup>2+</sup> and GIP mRNA level. In addition, TSE1 upregulated the protein expression of Takeda G protein-coupled receptor 5 (TGR5), and TGR5 siRNA significantly decreased GIP expression in TSE1-treated STC-1 cells. Network pharmacology analysis revealed that six proteins and five signaling pathways were associated with SGLT1, TGR5 and GIP regulated by TSE1. Taken together, tea seed saponins could stimulate GIP expression via SGLT1 and TGR5, and were promising natural active ingredients for improving metabolism and related diseases. |
| first_indexed | 2024-03-09T09:51:29Z |
| format | Article |
| id | doaj.art-fa08629592ae4f918afc368659edaae3 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T09:51:29Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-fa08629592ae4f918afc368659edaae32023-12-02T00:07:31ZengMDPI AGNutrients2072-66432022-08-011416341310.3390/nu14163413Saponins from <i>Camellia sinensis</i> Seeds Stimulate GIP Secretion in Mice and STC-1 Cells via SGLT1 and TGR5Huanqing Zhu0Kaixi Wang1Shuna Chen2Jiaxin Kang3Na Guo4Hongbo Chen5Junsheng Liu6Yuanyuan Wu7Puming He8Youying Tu9Bo Li10Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang Shuren University, 8 Shuren Road, Hangzhou 310000, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaGlucose-dependent insulinotropic polypeptide (GIP) is one of the important incretins and possesses lots of physiological activities such as stimulating insulin secretion and maintaining glucose homeostasis. The pentacyclic triterpenoid saponins are the major active ingredients in tea (<i>Camellia sinensis</i>) seeds. This study aimed to investigate the effect of tea seed saponins on the GIP secretion and related mechanisms. Our data showed that the total tea seed saponins (TSS, 65 mg/kg BW) and theasaponin E<sub>1</sub> (TSE1, 2–4 µM) could increase the GIP mRNA and protein levels in mice and STC-1 cells. Phlorizin, the inhibitor of Sodium/glucose cotransporter 1 (SGLT1), reversed the TSE1-induced increase in Ca<sup>2+</sup> and GIP mRNA level. In addition, TSE1 upregulated the protein expression of Takeda G protein-coupled receptor 5 (TGR5), and TGR5 siRNA significantly decreased GIP expression in TSE1-treated STC-1 cells. Network pharmacology analysis revealed that six proteins and five signaling pathways were associated with SGLT1, TGR5 and GIP regulated by TSE1. Taken together, tea seed saponins could stimulate GIP expression via SGLT1 and TGR5, and were promising natural active ingredients for improving metabolism and related diseases.https://www.mdpi.com/2072-6643/14/16/3413tea seedSaponinTheasaponin E<sub>1</sub>GIPSGLT1TGR5 |
| spellingShingle | Huanqing Zhu Kaixi Wang Shuna Chen Jiaxin Kang Na Guo Hongbo Chen Junsheng Liu Yuanyuan Wu Puming He Youying Tu Bo Li Saponins from <i>Camellia sinensis</i> Seeds Stimulate GIP Secretion in Mice and STC-1 Cells via SGLT1 and TGR5 Nutrients tea seed Saponin Theasaponin E<sub>1</sub> GIP SGLT1 TGR5 |
| title | Saponins from <i>Camellia sinensis</i> Seeds Stimulate GIP Secretion in Mice and STC-1 Cells via SGLT1 and TGR5 |
| title_full | Saponins from <i>Camellia sinensis</i> Seeds Stimulate GIP Secretion in Mice and STC-1 Cells via SGLT1 and TGR5 |
| title_fullStr | Saponins from <i>Camellia sinensis</i> Seeds Stimulate GIP Secretion in Mice and STC-1 Cells via SGLT1 and TGR5 |
| title_full_unstemmed | Saponins from <i>Camellia sinensis</i> Seeds Stimulate GIP Secretion in Mice and STC-1 Cells via SGLT1 and TGR5 |
| title_short | Saponins from <i>Camellia sinensis</i> Seeds Stimulate GIP Secretion in Mice and STC-1 Cells via SGLT1 and TGR5 |
| title_sort | saponins from i camellia sinensis i seeds stimulate gip secretion in mice and stc 1 cells via sglt1 and tgr5 |
| topic | tea seed Saponin Theasaponin E<sub>1</sub> GIP SGLT1 TGR5 |
| url | https://www.mdpi.com/2072-6643/14/16/3413 |
| work_keys_str_mv | AT huanqingzhu saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT kaixiwang saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT shunachen saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT jiaxinkang saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT naguo saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT hongbochen saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT junshengliu saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT yuanyuanwu saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT puminghe saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT youyingtu saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 AT boli saponinsfromicamelliasinensisiseedsstimulategipsecretioninmiceandstc1cellsviasglt1andtgr5 |