Genomic attributes of airway commensal bacteria and mucosa
Abstract Microbial communities at the airway mucosal barrier are conserved and highly ordered, in likelihood reflecting co-evolution with human host factors. Freed of selection to digest nutrients, the airway microbiome underpins cognate management of mucosal immunity and pathogen resistance. We sho...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-05840-3 |
| _version_ | 1827326552751210496 |
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| author | Leah Cuthbertson Ulrike Löber Jonathan S. Ish-Horowicz Claire N. McBrien Colin Churchward Jeremy C. Parker Michael T. Olanipekun Conor Burke Aisling McGowan Gwyneth A. Davies Keir E. Lewis Julian M. Hopkin Kian Fan Chung Orla O’Carroll John Faul Joy Creaser-Thomas Mark Andrews Robin Ghosal Stefan Piatek Saffron A. G. Willis-Owen Theda U. P. Bartolomaeus Till Birkner Sarah Dwyer Nitin Kumar Elena M. Turek A. William Musk Jennie Hui Michael Hunter Alan James Marc-Emmanuel Dumas Sarah Filippi Michael J. Cox Trevor D. Lawley Sofia K. Forslund Miriam F. Moffatt William. O. C. Cookson |
| author_facet | Leah Cuthbertson Ulrike Löber Jonathan S. Ish-Horowicz Claire N. McBrien Colin Churchward Jeremy C. Parker Michael T. Olanipekun Conor Burke Aisling McGowan Gwyneth A. Davies Keir E. Lewis Julian M. Hopkin Kian Fan Chung Orla O’Carroll John Faul Joy Creaser-Thomas Mark Andrews Robin Ghosal Stefan Piatek Saffron A. G. Willis-Owen Theda U. P. Bartolomaeus Till Birkner Sarah Dwyer Nitin Kumar Elena M. Turek A. William Musk Jennie Hui Michael Hunter Alan James Marc-Emmanuel Dumas Sarah Filippi Michael J. Cox Trevor D. Lawley Sofia K. Forslund Miriam F. Moffatt William. O. C. Cookson |
| author_sort | Leah Cuthbertson |
| collection | DOAJ |
| description | Abstract Microbial communities at the airway mucosal barrier are conserved and highly ordered, in likelihood reflecting co-evolution with human host factors. Freed of selection to digest nutrients, the airway microbiome underpins cognate management of mucosal immunity and pathogen resistance. We show here the initial results of systematic culture and whole-genome sequencing of the thoracic airway bacteria, identifying 52 novel species amongst 126 organisms that constitute 75% of commensals typically present in heathy individuals. Clinically relevant genes encode antimicrobial synthesis, adhesion and biofilm formation, immune modulation, iron utilisation, nitrous oxide (NO) metabolism and sphingolipid signalling. Using whole-genome content we identify dysbiotic features that may influence asthma and chronic obstructive pulmonary disease. We match isolate gene content to transcripts and metabolites expressed late in airway epithelial differentiation, identifying pathways to sustain host interactions with microbiota. Our results provide a systematic basis for decrypting interactions between commensals, pathogens, and mucosa in lung diseases of global significance. |
| first_indexed | 2024-03-07T14:46:14Z |
| format | Article |
| id | doaj.art-fa0a86718fb4447f9b20ab7e4ec9f322 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-07T14:46:14Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-fa0a86718fb4447f9b20ab7e4ec9f3222024-03-05T19:59:45ZengNature PortfolioCommunications Biology2399-36422024-02-017111410.1038/s42003-024-05840-3Genomic attributes of airway commensal bacteria and mucosaLeah Cuthbertson0Ulrike Löber1Jonathan S. Ish-Horowicz2Claire N. McBrien3Colin Churchward4Jeremy C. Parker5Michael T. Olanipekun6Conor Burke7Aisling McGowan8Gwyneth A. Davies9Keir E. Lewis10Julian M. Hopkin11Kian Fan Chung12Orla O’Carroll13John Faul14Joy Creaser-Thomas15Mark Andrews16Robin Ghosal17Stefan Piatek18Saffron A. G. Willis-Owen19Theda U. P. Bartolomaeus20Till Birkner21Sarah Dwyer22Nitin Kumar23Elena M. Turek24A. William Musk25Jennie Hui26Michael Hunter27Alan James28Marc-Emmanuel Dumas29Sarah Filippi30Michael J. Cox31Trevor D. Lawley32Sofia K. Forslund33Miriam F. Moffatt34William. O. C. Cookson35National Heart and Lung Institute, Imperial College LondonMax Delbrück Center for Molecular Medicine (MDC)National Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonDepartment of Respiratory Medicine, Connolly HospitalDepartment of Respiratory Medicine, Connolly HospitalPopulation Data Science and Health Data Research UK BREATHE Hub, Swansea University Medical School, Swansea UniversityCollege of Medicine, Institute of Life Science, Swansea UniversityCollege of Medicine, Institute of Life Science, Swansea UniversityNational Heart and Lung Institute, Imperial College LondonDepartment of Respiratory Medicine, Connolly HospitalDepartment of Respiratory Medicine, Connolly HospitalCollege of Medicine, Institute of Life Science, Swansea UniversityRespiratory Medicine, Hywel Dda University Health BoardRespiratory Medicine, Hywel Dda University Health BoardNational Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonMax Delbrück Center for Molecular Medicine (MDC)Max Delbrück Center for Molecular Medicine (MDC)National Heart and Lung Institute, Imperial College LondonHost-Microbiota Interactions Laboratory, Wellcome Sanger Institute, Wellcome Genome CampusNational Heart and Lung Institute, Imperial College LondonSchool of Population and Global Health, The University of Western AustraliaSchool of Population and Global Health, The University of Western AustraliaSchool of Population and Global Health, The University of Western AustraliaSchool of Population and Global Health, The University of Western AustraliaNational Heart and Lung Institute, Imperial College LondonDepartment of Mathematics, Imperial College LondonUniversity of Birmingham College of Medical and Dental Sciences, 150183, Institute of Microbiology and InfectionHost-Microbiota Interactions Laboratory, Wellcome Sanger Institute, Wellcome Genome CampusMax Delbrück Center for Molecular Medicine (MDC)National Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonAbstract Microbial communities at the airway mucosal barrier are conserved and highly ordered, in likelihood reflecting co-evolution with human host factors. Freed of selection to digest nutrients, the airway microbiome underpins cognate management of mucosal immunity and pathogen resistance. We show here the initial results of systematic culture and whole-genome sequencing of the thoracic airway bacteria, identifying 52 novel species amongst 126 organisms that constitute 75% of commensals typically present in heathy individuals. Clinically relevant genes encode antimicrobial synthesis, adhesion and biofilm formation, immune modulation, iron utilisation, nitrous oxide (NO) metabolism and sphingolipid signalling. Using whole-genome content we identify dysbiotic features that may influence asthma and chronic obstructive pulmonary disease. We match isolate gene content to transcripts and metabolites expressed late in airway epithelial differentiation, identifying pathways to sustain host interactions with microbiota. Our results provide a systematic basis for decrypting interactions between commensals, pathogens, and mucosa in lung diseases of global significance.https://doi.org/10.1038/s42003-024-05840-3 |
| spellingShingle | Leah Cuthbertson Ulrike Löber Jonathan S. Ish-Horowicz Claire N. McBrien Colin Churchward Jeremy C. Parker Michael T. Olanipekun Conor Burke Aisling McGowan Gwyneth A. Davies Keir E. Lewis Julian M. Hopkin Kian Fan Chung Orla O’Carroll John Faul Joy Creaser-Thomas Mark Andrews Robin Ghosal Stefan Piatek Saffron A. G. Willis-Owen Theda U. P. Bartolomaeus Till Birkner Sarah Dwyer Nitin Kumar Elena M. Turek A. William Musk Jennie Hui Michael Hunter Alan James Marc-Emmanuel Dumas Sarah Filippi Michael J. Cox Trevor D. Lawley Sofia K. Forslund Miriam F. Moffatt William. O. C. Cookson Genomic attributes of airway commensal bacteria and mucosa Communications Biology |
| title | Genomic attributes of airway commensal bacteria and mucosa |
| title_full | Genomic attributes of airway commensal bacteria and mucosa |
| title_fullStr | Genomic attributes of airway commensal bacteria and mucosa |
| title_full_unstemmed | Genomic attributes of airway commensal bacteria and mucosa |
| title_short | Genomic attributes of airway commensal bacteria and mucosa |
| title_sort | genomic attributes of airway commensal bacteria and mucosa |
| url | https://doi.org/10.1038/s42003-024-05840-3 |
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