Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum
Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. For CEA detection, a novel type of metal–organic framework (MOF) was prepared by grafting CEA aptamer-incorporated DNA tetrahedral (TDN) nanostructures into PCN-222 (Fe)-based MOF (referred as CEA<sub>apt</sub>-T...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2310-2861/7/4/181 |
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| author | Lingjun Sha Mingcong Zhu Fuqing Lin Xiaomeng Yu Langjian Dong Licheng Wu Rong Ding Shuai Wu Jingjing Xu |
| author_facet | Lingjun Sha Mingcong Zhu Fuqing Lin Xiaomeng Yu Langjian Dong Licheng Wu Rong Ding Shuai Wu Jingjing Xu |
| author_sort | Lingjun Sha |
| collection | DOAJ |
| description | Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. For CEA detection, a novel type of metal–organic framework (MOF) was prepared by grafting CEA aptamer-incorporated DNA tetrahedral (TDN) nanostructures into PCN-222 (Fe)-based MOF (referred as CEA<sub>apt</sub>-TDN-MOF colloid nanorods). The synthesized CEA<sub>apt</sub>-TDN-MOF is a very stable detection system due to the vertex phosphorylated TDN structure at the interface, possessing a one-year shelf-life. Moreover, it exhibits a significant horseradish peroxidase mimicking activity due to the iron porphyrin ring, which leads to a colorimetric reaction upon binding toward antibody-captured CEA. Using this method, we successfully achieved the highly specific and ultra-sensitive detection of CEA with a limit of detection as low as 3.3 pg/mL. In addition, this method can detect and analyze the target proteins in clinical serum samples, effectively identify the difference between normal individuals and patients with colon cancer, and provide a new method for the clinical diagnosis of tumors, demonstrating a great application potential. |
| first_indexed | 2024-03-10T04:03:53Z |
| format | Article |
| id | doaj.art-fa10b843eb09426d871f665122dba439 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T04:03:53Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
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| series | Gels |
| spelling | doaj.art-fa10b843eb09426d871f665122dba4392023-11-23T08:27:36ZengMDPI AGGels2310-28612021-10-017418110.3390/gels7040181Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in SerumLingjun Sha0Mingcong Zhu1Fuqing Lin2Xiaomeng Yu3Langjian Dong4Licheng Wu5Rong Ding6Shuai Wu7Jingjing Xu8Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaSino-European School of Technology of Shanghai University, Shanghai University, Shanghai 200444, ChinaSchool of Basic Medical Sciences, Fudan University, Shanghai 200433, ChinaCenter for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaCenter for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaSino-European School of Technology of Shanghai University, Shanghai University, Shanghai 200444, ChinaSino-European School of Technology of Shanghai University, Shanghai University, Shanghai 200444, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaCenter for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaCarcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. For CEA detection, a novel type of metal–organic framework (MOF) was prepared by grafting CEA aptamer-incorporated DNA tetrahedral (TDN) nanostructures into PCN-222 (Fe)-based MOF (referred as CEA<sub>apt</sub>-TDN-MOF colloid nanorods). The synthesized CEA<sub>apt</sub>-TDN-MOF is a very stable detection system due to the vertex phosphorylated TDN structure at the interface, possessing a one-year shelf-life. Moreover, it exhibits a significant horseradish peroxidase mimicking activity due to the iron porphyrin ring, which leads to a colorimetric reaction upon binding toward antibody-captured CEA. Using this method, we successfully achieved the highly specific and ultra-sensitive detection of CEA with a limit of detection as low as 3.3 pg/mL. In addition, this method can detect and analyze the target proteins in clinical serum samples, effectively identify the difference between normal individuals and patients with colon cancer, and provide a new method for the clinical diagnosis of tumors, demonstrating a great application potential.https://www.mdpi.com/2310-2861/7/4/181colloid nanorodsDNA tetrahedron nanostructurecatalytic oxidationcarcinoembryonic antigencolorimetric detection |
| spellingShingle | Lingjun Sha Mingcong Zhu Fuqing Lin Xiaomeng Yu Langjian Dong Licheng Wu Rong Ding Shuai Wu Jingjing Xu Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum Gels colloid nanorods DNA tetrahedron nanostructure catalytic oxidation carcinoembryonic antigen colorimetric detection |
| title | Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum |
| title_full | Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum |
| title_fullStr | Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum |
| title_full_unstemmed | Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum |
| title_short | Stable DNA Aptamer–Metal–Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum |
| title_sort | stable dna aptamer metal organic framework as horseradish peroxidase mimic for ultra sensitive detection of carcinoembryonic antigen in serum |
| topic | colloid nanorods DNA tetrahedron nanostructure catalytic oxidation carcinoembryonic antigen colorimetric detection |
| url | https://www.mdpi.com/2310-2861/7/4/181 |
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