Evaluating the role of SARS-CoV-2 target genes based on two nucleic acid assay kits
BackgroundEffective isolation and early treatment of coronavirus disease 2019 (COVID-19) relies on rapid, accurate, and straightforward diagnostic tools. In response to the rapidly increasing number of cases, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays for multiple...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2022.982171/full |
| _version_ | 1811206267933294592 |
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| author | Xuetong Zhu Xuetong Zhu Fengyan Zhou Qi Zhou Qi Zhou Jiancheng Xu Jiancheng Xu |
| author_facet | Xuetong Zhu Xuetong Zhu Fengyan Zhou Qi Zhou Qi Zhou Jiancheng Xu Jiancheng Xu |
| author_sort | Xuetong Zhu |
| collection | DOAJ |
| description | BackgroundEffective isolation and early treatment of coronavirus disease 2019 (COVID-19) relies on rapid, accurate, and straightforward diagnostic tools. In response to the rapidly increasing number of cases, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays for multiple target genes have become widely available in the market.MethodsIn total, 236 COVID-19 patients with positive results in both RT-qPCR and rapid antigen diagnosis (Ag-RDT) were enrolled in the study. The cycle threshold (Ct) was compared with different onset times and target genes. Comparison between groups was evaluated with the Kruskal-Wallis test and Dunn test. The correlation between target genes was analyzed by Spearman.ResultsIn samples of Ct ≤ 21, Ct was different for the nucleocapsid (N), open reading frame 1ab (ORF1ab), and envelope (E) genes (P < 0.05). Mild COVID-19 patients within 7 days of onset accounted for 67.80% of all enrolled patients. At the above stage, all target genes reached the trough of Ct, and N genes showed lower values than the other target genes. The Ct of the ORF1ab and N gene in asymptomatic patients differed from those of mild patients within 7 days and more than 14 days of onset. The kits used in the study showed strong consistency among target genes, with all correlation coefficients >0.870.ConclusionRT-qPCR confirmed that the N gene performed well in Ct ≤ 21 and samples within 7 days of onset. Ag-RDT was discriminatory for patients within 7 days of onset. This study facilitated early identification and control of COVID-19 prevalence among patients. |
| first_indexed | 2024-04-12T03:44:50Z |
| format | Article |
| id | doaj.art-fa138e7b084147b7847a629429f9a7eb |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-04-12T03:44:50Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj.art-fa138e7b084147b7847a629429f9a7eb2022-12-22T03:49:11ZengFrontiers Media S.A.Frontiers in Public Health2296-25652022-09-011010.3389/fpubh.2022.982171982171Evaluating the role of SARS-CoV-2 target genes based on two nucleic acid assay kitsXuetong Zhu0Xuetong Zhu1Fengyan Zhou2Qi Zhou3Qi Zhou4Jiancheng Xu5Jiancheng Xu6Center of Infectious Diseases and Pathogen Biology, The First Hospital of Jilin University, Changchun, ChinaDepartment of Laboratory Medicine, The First Hospital of Jilin University, Changchun, ChinaDepartment of Infectious Disease Control, Jilin City Center for Disease Control and Prevention, Jilin, ChinaCenter of Infectious Diseases and Pathogen Biology, The First Hospital of Jilin University, Changchun, ChinaDepartment of Pediatrics, The First Hospital of Jilin University, Changchun, ChinaCenter of Infectious Diseases and Pathogen Biology, The First Hospital of Jilin University, Changchun, ChinaDepartment of Laboratory Medicine, The First Hospital of Jilin University, Changchun, ChinaBackgroundEffective isolation and early treatment of coronavirus disease 2019 (COVID-19) relies on rapid, accurate, and straightforward diagnostic tools. In response to the rapidly increasing number of cases, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays for multiple target genes have become widely available in the market.MethodsIn total, 236 COVID-19 patients with positive results in both RT-qPCR and rapid antigen diagnosis (Ag-RDT) were enrolled in the study. The cycle threshold (Ct) was compared with different onset times and target genes. Comparison between groups was evaluated with the Kruskal-Wallis test and Dunn test. The correlation between target genes was analyzed by Spearman.ResultsIn samples of Ct ≤ 21, Ct was different for the nucleocapsid (N), open reading frame 1ab (ORF1ab), and envelope (E) genes (P < 0.05). Mild COVID-19 patients within 7 days of onset accounted for 67.80% of all enrolled patients. At the above stage, all target genes reached the trough of Ct, and N genes showed lower values than the other target genes. The Ct of the ORF1ab and N gene in asymptomatic patients differed from those of mild patients within 7 days and more than 14 days of onset. The kits used in the study showed strong consistency among target genes, with all correlation coefficients >0.870.ConclusionRT-qPCR confirmed that the N gene performed well in Ct ≤ 21 and samples within 7 days of onset. Ag-RDT was discriminatory for patients within 7 days of onset. This study facilitated early identification and control of COVID-19 prevalence among patients.https://www.frontiersin.org/articles/10.3389/fpubh.2022.982171/fullcoronavirus disease 2019severe acute respiratory syndrome coronavirus 2antigen rapid diagnostic testreverse transcription-quantitative polymerase chain reactionnucleocapsid gene |
| spellingShingle | Xuetong Zhu Xuetong Zhu Fengyan Zhou Qi Zhou Qi Zhou Jiancheng Xu Jiancheng Xu Evaluating the role of SARS-CoV-2 target genes based on two nucleic acid assay kits Frontiers in Public Health coronavirus disease 2019 severe acute respiratory syndrome coronavirus 2 antigen rapid diagnostic test reverse transcription-quantitative polymerase chain reaction nucleocapsid gene |
| title | Evaluating the role of SARS-CoV-2 target genes based on two nucleic acid assay kits |
| title_full | Evaluating the role of SARS-CoV-2 target genes based on two nucleic acid assay kits |
| title_fullStr | Evaluating the role of SARS-CoV-2 target genes based on two nucleic acid assay kits |
| title_full_unstemmed | Evaluating the role of SARS-CoV-2 target genes based on two nucleic acid assay kits |
| title_short | Evaluating the role of SARS-CoV-2 target genes based on two nucleic acid assay kits |
| title_sort | evaluating the role of sars cov 2 target genes based on two nucleic acid assay kits |
| topic | coronavirus disease 2019 severe acute respiratory syndrome coronavirus 2 antigen rapid diagnostic test reverse transcription-quantitative polymerase chain reaction nucleocapsid gene |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2022.982171/full |
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