Dynamic Tissue—Specific Transcriptome Changes in Response to <i>Verticillium dahliae</i> in Wild Mint Species <i>Mentha longifolia</i>
<i>Mentha longifolia</i> is a wild mint species being used as a model to study the genetics of resistance to the fungal wilt pathogen <i>Verticillium dahliae</i>. We used high-throughput Illumina sequencing to study gene expression in response to <i>V. dahliae</i>...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/11/5/674 |
| _version_ | 1797474132952088576 |
|---|---|
| author | Kelly J. Vining Iovanna Pandelova |
| author_facet | Kelly J. Vining Iovanna Pandelova |
| author_sort | Kelly J. Vining |
| collection | DOAJ |
| description | <i>Mentha longifolia</i> is a wild mint species being used as a model to study the genetics of resistance to the fungal wilt pathogen <i>Verticillium dahliae</i>. We used high-throughput Illumina sequencing to study gene expression in response to <i>V. dahliae</i> inoculation in two <i>M. longifolia</i> USDA accessions with contrasting phenotypes: wilt-resistant CMEN 585 and wilt-susceptible CMEN 584. Roots and stems were sampled at two early post-inoculation time points, four hours and twenty-four hours, and again at ten days and twenty days post-inoculation. Overall, many more genes were differentially-regulated in wilt-resistant CMEN 585 than in wilt-susceptible CMEN 584. The greatest numbers of differentially expressed genes were found in the roots of CMEN 585 at the early time points. Specific genes exhibiting early, strong upregulation in roots of CMEN 585 but not in CMEN 584 included homologs of known plant defense response genes as well as genes involved in monoterpene biosynthesis. These genes were also upregulated in stems at the later time points. This study provides a comprehensive view of transcription reprogramming in Verticillium wilt-resistant mint, which will be the basis for further study and for molecular marker development. |
| first_indexed | 2024-03-09T20:25:48Z |
| format | Article |
| id | doaj.art-fa1588860b4f41128b8aeea1c690e85c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T20:25:48Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-fa1588860b4f41128b8aeea1c690e85c2023-11-23T23:36:28ZengMDPI AGPlants2223-77472022-03-0111567410.3390/plants11050674Dynamic Tissue—Specific Transcriptome Changes in Response to <i>Verticillium dahliae</i> in Wild Mint Species <i>Mentha longifolia</i>Kelly J. Vining0Iovanna Pandelova1Department of Horticulture, Oregon State University, Corvallis, OR 97331, USADepartment of Horticulture, Oregon State University, Corvallis, OR 97331, USA<i>Mentha longifolia</i> is a wild mint species being used as a model to study the genetics of resistance to the fungal wilt pathogen <i>Verticillium dahliae</i>. We used high-throughput Illumina sequencing to study gene expression in response to <i>V. dahliae</i> inoculation in two <i>M. longifolia</i> USDA accessions with contrasting phenotypes: wilt-resistant CMEN 585 and wilt-susceptible CMEN 584. Roots and stems were sampled at two early post-inoculation time points, four hours and twenty-four hours, and again at ten days and twenty days post-inoculation. Overall, many more genes were differentially-regulated in wilt-resistant CMEN 585 than in wilt-susceptible CMEN 584. The greatest numbers of differentially expressed genes were found in the roots of CMEN 585 at the early time points. Specific genes exhibiting early, strong upregulation in roots of CMEN 585 but not in CMEN 584 included homologs of known plant defense response genes as well as genes involved in monoterpene biosynthesis. These genes were also upregulated in stems at the later time points. This study provides a comprehensive view of transcription reprogramming in Verticillium wilt-resistant mint, which will be the basis for further study and for molecular marker development.https://www.mdpi.com/2223-7747/11/5/674menthamintverticilliumdisease resistancewiltmonoterpene |
| spellingShingle | Kelly J. Vining Iovanna Pandelova Dynamic Tissue—Specific Transcriptome Changes in Response to <i>Verticillium dahliae</i> in Wild Mint Species <i>Mentha longifolia</i> Plants mentha mint verticillium disease resistance wilt monoterpene |
| title | Dynamic Tissue—Specific Transcriptome Changes in Response to <i>Verticillium dahliae</i> in Wild Mint Species <i>Mentha longifolia</i> |
| title_full | Dynamic Tissue—Specific Transcriptome Changes in Response to <i>Verticillium dahliae</i> in Wild Mint Species <i>Mentha longifolia</i> |
| title_fullStr | Dynamic Tissue—Specific Transcriptome Changes in Response to <i>Verticillium dahliae</i> in Wild Mint Species <i>Mentha longifolia</i> |
| title_full_unstemmed | Dynamic Tissue—Specific Transcriptome Changes in Response to <i>Verticillium dahliae</i> in Wild Mint Species <i>Mentha longifolia</i> |
| title_short | Dynamic Tissue—Specific Transcriptome Changes in Response to <i>Verticillium dahliae</i> in Wild Mint Species <i>Mentha longifolia</i> |
| title_sort | dynamic tissue specific transcriptome changes in response to i verticillium dahliae i in wild mint species i mentha longifolia i |
| topic | mentha mint verticillium disease resistance wilt monoterpene |
| url | https://www.mdpi.com/2223-7747/11/5/674 |
| work_keys_str_mv | AT kellyjvining dynamictissuespecifictranscriptomechangesinresponsetoiverticilliumdahliaeiinwildmintspeciesimenthalongifoliai AT iovannapandelova dynamictissuespecifictranscriptomechangesinresponsetoiverticilliumdahliaeiinwildmintspeciesimenthalongifoliai |