Alleviation of Oxidative Damage Induced by CaCl2 Priming Is Related to Osmotic and Ion Stress Reduction Rather Than Enhanced Antioxidant Capacity During Germination Under Salt Stress in Sorghum

Seed germination is the sensitive period to salt stress. Calcium chloride (CaCl2) has been proved as an effective priming agent which can promote the sorghum germination under salt stress. However, there are few reports on CaCl2 priming to improve the salt tolerance during seed germination. The pres...

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Main Authors: Xiaofei Chen, Ruidong Zhang, Bang Li, Tong Cui, Chang Liu, Chunjuan Liu, Bingru Chen, Yufei Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-04-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.881039/full
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author Xiaofei Chen
Xiaofei Chen
Ruidong Zhang
Ruidong Zhang
Bang Li
Tong Cui
Chang Liu
Chunjuan Liu
Bingru Chen
Yufei Zhou
author_facet Xiaofei Chen
Xiaofei Chen
Ruidong Zhang
Ruidong Zhang
Bang Li
Tong Cui
Chang Liu
Chunjuan Liu
Bingru Chen
Yufei Zhou
author_sort Xiaofei Chen
collection DOAJ
description Seed germination is the sensitive period to salt stress. Calcium chloride (CaCl2) has been proved as an effective priming agent which can promote the sorghum germination under salt stress. However, there are few reports on CaCl2 priming to improve the salt tolerance during seed germination. The present study investigated the effects of CaCl2 priming on sorghum germination, antioxidant metabolism, osmotic regulation and ion balance under salt stress (150 mM NaCl). The results revealed that the salt stress inhibited the elongation of mesocotyl and root and reduced the germination rate of sorghum. While CaCl2 priming significantly promoted the elongation of mesocotyl and root, and increased the germination rate of sorghum under salt stress. CaCl2 priming notably increased the content of osmotic substances in mesocotyl and root of sorghum under salt stress, and increased the relative water content in these tissues. CaCl2 priming decreased Na+ content and increased K+, Ca2+ contents and the K+/ Na+ in mesocotyl and root, such effects might be induced by up-regulating the expression of NHX2, NHX4, SOS1, AKT1, AKT2, HKT1, HAK1, and KUP. CaCl2 priming reduced the antioxidant enzymes activities and related gene expression compared with untreated sorghum seeds under salt stress. In short, CaCl2 priming improved sorghum germination by enhancing osmotic regulation and ion balance instead of antioxidant enzyme activity. However, the molecular mechanisms of Ca2+ signaling induced by CaCl2 priming in association with the enhanced germination in primed sorghum seeds under salt stress need to be addressed in future studies.
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spelling doaj.art-fa15c232c080447682ca1acb05ba3da42022-12-22T01:05:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-04-011310.3389/fpls.2022.881039881039Alleviation of Oxidative Damage Induced by CaCl2 Priming Is Related to Osmotic and Ion Stress Reduction Rather Than Enhanced Antioxidant Capacity During Germination Under Salt Stress in SorghumXiaofei Chen0Xiaofei Chen1Ruidong Zhang2Ruidong Zhang3Bang Li4Tong Cui5Chang Liu6Chunjuan Liu7Bingru Chen8Yufei Zhou9College of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCrop Research Institute, Anhui Academy of Agricultural Sciences, Hefei, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaInstitute of Economic Crop, Shanxi Academy of Agricultural Sciences, Fenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaInstitute of Crop Germplasm Resources, Jilin Academy of Agricultural Sciences, Changchun, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaSeed germination is the sensitive period to salt stress. Calcium chloride (CaCl2) has been proved as an effective priming agent which can promote the sorghum germination under salt stress. However, there are few reports on CaCl2 priming to improve the salt tolerance during seed germination. The present study investigated the effects of CaCl2 priming on sorghum germination, antioxidant metabolism, osmotic regulation and ion balance under salt stress (150 mM NaCl). The results revealed that the salt stress inhibited the elongation of mesocotyl and root and reduced the germination rate of sorghum. While CaCl2 priming significantly promoted the elongation of mesocotyl and root, and increased the germination rate of sorghum under salt stress. CaCl2 priming notably increased the content of osmotic substances in mesocotyl and root of sorghum under salt stress, and increased the relative water content in these tissues. CaCl2 priming decreased Na+ content and increased K+, Ca2+ contents and the K+/ Na+ in mesocotyl and root, such effects might be induced by up-regulating the expression of NHX2, NHX4, SOS1, AKT1, AKT2, HKT1, HAK1, and KUP. CaCl2 priming reduced the antioxidant enzymes activities and related gene expression compared with untreated sorghum seeds under salt stress. In short, CaCl2 priming improved sorghum germination by enhancing osmotic regulation and ion balance instead of antioxidant enzyme activity. However, the molecular mechanisms of Ca2+ signaling induced by CaCl2 priming in association with the enhanced germination in primed sorghum seeds under salt stress need to be addressed in future studies.https://www.frontiersin.org/articles/10.3389/fpls.2022.881039/fullsorghum germinationseed primingsaline stressosmotic adjustmention transportcalcium signaling
spellingShingle Xiaofei Chen
Xiaofei Chen
Ruidong Zhang
Ruidong Zhang
Bang Li
Tong Cui
Chang Liu
Chunjuan Liu
Bingru Chen
Yufei Zhou
Alleviation of Oxidative Damage Induced by CaCl2 Priming Is Related to Osmotic and Ion Stress Reduction Rather Than Enhanced Antioxidant Capacity During Germination Under Salt Stress in Sorghum
Frontiers in Plant Science
sorghum germination
seed priming
saline stress
osmotic adjustment
ion transport
calcium signaling
title Alleviation of Oxidative Damage Induced by CaCl2 Priming Is Related to Osmotic and Ion Stress Reduction Rather Than Enhanced Antioxidant Capacity During Germination Under Salt Stress in Sorghum
title_full Alleviation of Oxidative Damage Induced by CaCl2 Priming Is Related to Osmotic and Ion Stress Reduction Rather Than Enhanced Antioxidant Capacity During Germination Under Salt Stress in Sorghum
title_fullStr Alleviation of Oxidative Damage Induced by CaCl2 Priming Is Related to Osmotic and Ion Stress Reduction Rather Than Enhanced Antioxidant Capacity During Germination Under Salt Stress in Sorghum
title_full_unstemmed Alleviation of Oxidative Damage Induced by CaCl2 Priming Is Related to Osmotic and Ion Stress Reduction Rather Than Enhanced Antioxidant Capacity During Germination Under Salt Stress in Sorghum
title_short Alleviation of Oxidative Damage Induced by CaCl2 Priming Is Related to Osmotic and Ion Stress Reduction Rather Than Enhanced Antioxidant Capacity During Germination Under Salt Stress in Sorghum
title_sort alleviation of oxidative damage induced by cacl2 priming is related to osmotic and ion stress reduction rather than enhanced antioxidant capacity during germination under salt stress in sorghum
topic sorghum germination
seed priming
saline stress
osmotic adjustment
ion transport
calcium signaling
url https://www.frontiersin.org/articles/10.3389/fpls.2022.881039/full
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