Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics
The tomato is a research model for fruit-ripening, however, its fruit-ripening mechanism still needs more extensive and in-depth exploration. Here, using TMT and LC-MS, the proteome and phosphoproteome of AC<sup>++</sup> (wild type) and <i>rin</i> (ripening-inhibitor) mutant...
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11782 |
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| author | Qiaoli Xie Yanling Tian Zongli Hu Lincheng Zhang Boyan Tang Yunshu Wang Jing Li Guoping Chen |
| author_facet | Qiaoli Xie Yanling Tian Zongli Hu Lincheng Zhang Boyan Tang Yunshu Wang Jing Li Guoping Chen |
| author_sort | Qiaoli Xie |
| collection | DOAJ |
| description | The tomato is a research model for fruit-ripening, however, its fruit-ripening mechanism still needs more extensive and in-depth exploration. Here, using TMT and LC-MS, the proteome and phosphoproteome of AC<sup>++</sup> (wild type) and <i>rin</i> (ripening-inhibitor) mutant fruits were studied to investigate the translation and post-translational regulation mechanisms of tomato fruit-ripening. A total of 6141 proteins and 4011 phosphorylation sites contained quantitative information. One-hundred proteins were identified in both omics’ profiles, which were mainly found in ethylene biosynthesis and signal transduction, photosynthesis regulation, carotenoid and flavonoid biosynthesis, chlorophyll degradation, ribosomal subunit expression changes, MAPK pathway, transcription factors and kinases. The affected protein levels were correlated with their corresponding gene transcript levels, such as NAC-NOR, MADS-RIN, IMA, TAGL1, MADS-MC and TDR4. Changes in the phosphorylation levels of NAC-NOR and IMA were involved in the regulation of tomato fruit-ripening. Although photosynthesis was inhibited, there were diverse primary and secondary metabolic pathways, such as glycolysis, fatty acid metabolism, vitamin metabolism and isoprenoid biosynthesis, regulated by phosphorylation. These data constitute a map of protein—protein phosphorylation in the regulation of tomato fruit-ripening, which lays the foundation for future in-depth study of the sophisticated molecular mechanisms of fruit-ripening and provide guidance for molecular breeding. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:01:15Z |
| publishDate | 2021-10-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fa15d337f28e4e17ab02da7e281d22a22023-11-22T20:57:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122211178210.3390/ijms222111782Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and PhosphoproteomicsQiaoli Xie0Yanling Tian1Zongli Hu2Lincheng Zhang3Boyan Tang4Yunshu Wang5Jing Li6Guoping Chen7Key Laboratory of Biorheological Science and Technology Ministry of Education, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology Ministry of Education, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology Ministry of Education, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology Ministry of Education, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology Ministry of Education, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology Ministry of Education, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology Ministry of Education, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology Ministry of Education, Chongqing University, Chongqing 400044, ChinaThe tomato is a research model for fruit-ripening, however, its fruit-ripening mechanism still needs more extensive and in-depth exploration. Here, using TMT and LC-MS, the proteome and phosphoproteome of AC<sup>++</sup> (wild type) and <i>rin</i> (ripening-inhibitor) mutant fruits were studied to investigate the translation and post-translational regulation mechanisms of tomato fruit-ripening. A total of 6141 proteins and 4011 phosphorylation sites contained quantitative information. One-hundred proteins were identified in both omics’ profiles, which were mainly found in ethylene biosynthesis and signal transduction, photosynthesis regulation, carotenoid and flavonoid biosynthesis, chlorophyll degradation, ribosomal subunit expression changes, MAPK pathway, transcription factors and kinases. The affected protein levels were correlated with their corresponding gene transcript levels, such as NAC-NOR, MADS-RIN, IMA, TAGL1, MADS-MC and TDR4. Changes in the phosphorylation levels of NAC-NOR and IMA were involved in the regulation of tomato fruit-ripening. Although photosynthesis was inhibited, there were diverse primary and secondary metabolic pathways, such as glycolysis, fatty acid metabolism, vitamin metabolism and isoprenoid biosynthesis, regulated by phosphorylation. These data constitute a map of protein—protein phosphorylation in the regulation of tomato fruit-ripening, which lays the foundation for future in-depth study of the sophisticated molecular mechanisms of fruit-ripening and provide guidance for molecular breeding.https://www.mdpi.com/1422-0067/22/21/11782proteomicsphosphoproteomicsfruit-ripening<i>rin</i>tomato (<i>Solanum lycopersicum</i>) |
| spellingShingle | Qiaoli Xie Yanling Tian Zongli Hu Lincheng Zhang Boyan Tang Yunshu Wang Jing Li Guoping Chen Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics International Journal of Molecular Sciences proteomics phosphoproteomics fruit-ripening <i>rin</i> tomato (<i>Solanum lycopersicum</i>) |
| title | Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics |
| title_full | Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics |
| title_fullStr | Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics |
| title_full_unstemmed | Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics |
| title_short | Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics |
| title_sort | novel translational and phosphorylation modification regulation mechanisms of tomato i solanum lycopersicum i fruit ripening revealed by integrative proteomics and phosphoproteomics |
| topic | proteomics phosphoproteomics fruit-ripening <i>rin</i> tomato (<i>Solanum lycopersicum</i>) |
| url | https://www.mdpi.com/1422-0067/22/21/11782 |
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