Morphological and Molecular Identification of <i>Physaloptera alata</i> (Nematoda: Spirurida) in a Booted Eagle (<i>Aquila pennata</i>) from Portugal
<i>Physaloptera</i> spp. are parasitic nematodes that infect the gastrointestinal tracts of many carnivores and omnivores. Although they are distributed worldwide, <i>Physaloptera</i> spp. have not been studied in raptors in Portugal. In this study, we report <i>Physalo...
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MDPI AG
2023-05-01
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| author | Zaida Rentería-Solís David W. Ramilo Ronald Schmäschke Sandra Gawlowska Jorge Correia Filipa Lopes Luís Madeira de Carvalho Luís Cardoso Isabel Pereira da Fonseca |
| author_facet | Zaida Rentería-Solís David W. Ramilo Ronald Schmäschke Sandra Gawlowska Jorge Correia Filipa Lopes Luís Madeira de Carvalho Luís Cardoso Isabel Pereira da Fonseca |
| author_sort | Zaida Rentería-Solís |
| collection | DOAJ |
| description | <i>Physaloptera</i> spp. are parasitic nematodes that infect the gastrointestinal tracts of many carnivores and omnivores. Although they are distributed worldwide, <i>Physaloptera</i> spp. have not been studied in raptors in Portugal. In this study, we report <i>Physaloptera alata</i> in a booted eagle (<i>Aquila pennata</i>) in Portugal. Adult nematodes were discovered in the gizzard of a young booted eagle, and morphological features were consistent with those of the genus <i>Physaloptera</i>. DNA was extracted and a PCR assay performed to amplify a region of the 18S small subunit of the ribosomal RNA gene and the cytochrome c oxidase subunit I gene. The resulting PCR products were Sanger-sequenced, and comparison with the available sequences in the GenBank database confirmed the initial morphological classification as <i>Physaloptera</i> sp. Phylogenetic analysis clustered the sequence within the <i>Physaloptera</i> group. The presence of this parasite in raptors from Portugal is of particular importance to wildlife rehabilitation centers, disease ecologists, and wildlife professionals. Furthermore, we produced a new genetic sequence and have added it to the GenBank database of parasites in birds of prey. |
| first_indexed | 2024-03-11T04:01:09Z |
| format | Article |
| id | doaj.art-fa164746da47412194b838bf36856550 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T04:01:09Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-fa164746da47412194b838bf368565502023-11-18T00:10:31ZengMDPI AGAnimals2076-26152023-05-011310166910.3390/ani13101669Morphological and Molecular Identification of <i>Physaloptera alata</i> (Nematoda: Spirurida) in a Booted Eagle (<i>Aquila pennata</i>) from PortugalZaida Rentería-Solís0David W. Ramilo1Ronald Schmäschke2Sandra Gawlowska3Jorge Correia4Filipa Lopes5Luís Madeira de Carvalho6Luís Cardoso7Isabel Pereira da Fonseca8Institute for Parasitology, Centre of Infection Medicine, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 35, 04103 Leipzig, GermanyCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisboa, PortugalInstitute for Parasitology, Centre of Infection Medicine, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 35, 04103 Leipzig, GermanyInstitute for Parasitology, Centre of Infection Medicine, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 35, 04103 Leipzig, GermanyCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisboa, PortugalCERAS—Centro de Estudos e Recuperação de Animais Selvagens, Quercus Associação Nacional de Conservação da Natureza, 6000-909 Castelo Branco, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisboa, PortugalAssociate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal<i>Physaloptera</i> spp. are parasitic nematodes that infect the gastrointestinal tracts of many carnivores and omnivores. Although they are distributed worldwide, <i>Physaloptera</i> spp. have not been studied in raptors in Portugal. In this study, we report <i>Physaloptera alata</i> in a booted eagle (<i>Aquila pennata</i>) in Portugal. Adult nematodes were discovered in the gizzard of a young booted eagle, and morphological features were consistent with those of the genus <i>Physaloptera</i>. DNA was extracted and a PCR assay performed to amplify a region of the 18S small subunit of the ribosomal RNA gene and the cytochrome c oxidase subunit I gene. The resulting PCR products were Sanger-sequenced, and comparison with the available sequences in the GenBank database confirmed the initial morphological classification as <i>Physaloptera</i> sp. Phylogenetic analysis clustered the sequence within the <i>Physaloptera</i> group. The presence of this parasite in raptors from Portugal is of particular importance to wildlife rehabilitation centers, disease ecologists, and wildlife professionals. Furthermore, we produced a new genetic sequence and have added it to the GenBank database of parasites in birds of prey.https://www.mdpi.com/2076-2615/13/10/1669<i>Aquila pennata</i>booted eaglenematode<i>Physaloptera alata</i>Portugal |
| spellingShingle | Zaida Rentería-Solís David W. Ramilo Ronald Schmäschke Sandra Gawlowska Jorge Correia Filipa Lopes Luís Madeira de Carvalho Luís Cardoso Isabel Pereira da Fonseca Morphological and Molecular Identification of <i>Physaloptera alata</i> (Nematoda: Spirurida) in a Booted Eagle (<i>Aquila pennata</i>) from Portugal Animals <i>Aquila pennata</i> booted eagle nematode <i>Physaloptera alata</i> Portugal |
| title | Morphological and Molecular Identification of <i>Physaloptera alata</i> (Nematoda: Spirurida) in a Booted Eagle (<i>Aquila pennata</i>) from Portugal |
| title_full | Morphological and Molecular Identification of <i>Physaloptera alata</i> (Nematoda: Spirurida) in a Booted Eagle (<i>Aquila pennata</i>) from Portugal |
| title_fullStr | Morphological and Molecular Identification of <i>Physaloptera alata</i> (Nematoda: Spirurida) in a Booted Eagle (<i>Aquila pennata</i>) from Portugal |
| title_full_unstemmed | Morphological and Molecular Identification of <i>Physaloptera alata</i> (Nematoda: Spirurida) in a Booted Eagle (<i>Aquila pennata</i>) from Portugal |
| title_short | Morphological and Molecular Identification of <i>Physaloptera alata</i> (Nematoda: Spirurida) in a Booted Eagle (<i>Aquila pennata</i>) from Portugal |
| title_sort | morphological and molecular identification of i physaloptera alata i nematoda spirurida in a booted eagle i aquila pennata i from portugal |
| topic | <i>Aquila pennata</i> booted eagle nematode <i>Physaloptera alata</i> Portugal |
| url | https://www.mdpi.com/2076-2615/13/10/1669 |
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