Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension
As a natural calcium resource, animal bone needs to be miniaturized to the nanoscale to improve palatability and absorption capacity. To explore the mechanism of high-pressure homogenization (HPH) in preparing natural bone aqueous nanosuspensions, the relationships between the changes in protein con...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/18/2869 |
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| author | Xue Li Zhifei He Jingbing Xu Chang Su Xu Xiao Ling Zhang Huanhuan Zhang Hongjun Li |
| author_facet | Xue Li Zhifei He Jingbing Xu Chang Su Xu Xiao Ling Zhang Huanhuan Zhang Hongjun Li |
| author_sort | Xue Li |
| collection | DOAJ |
| description | As a natural calcium resource, animal bone needs to be miniaturized to the nanoscale to improve palatability and absorption capacity. To explore the mechanism of high-pressure homogenization (HPH) in preparing natural bone aqueous nanosuspensions, the relationships between the changes in protein conformation, solubility and quality characteristics of rabbit bone aqueous suspensions (RBAS) prepared by different HPH cycles were studied. The results showed that the improvements in particle size, stability and calcium solubility of RBASs could be mainly attributed to the improvement of protein solubility induced by the changes in protein conformation. HPH treatment led to the denaturation and degradation of protein in rabbit bone, generating soluble peptides and improving the stability of the suspensions by enhancing the surface charge of the particles. When collagen as the main protein was partially degraded, the hydroxyapatite in the bone was crushed into tiny particles. The increase in the particle-specific surface area led to the release of calcium ions, which chelated with the peptides to produce peptide calcium. However, excessive HPH treatment caused the production of protein macromolecular aggregates and affected the quality of RBASs. This study is helpful to promote the application of HPH technology in animal bone nanoprocessing. |
| first_indexed | 2024-03-10T00:02:52Z |
| format | Article |
| id | doaj.art-fa1a84dfa0994eed9b74076a9589bf9c |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T00:02:52Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-fa1a84dfa0994eed9b74076a9589bf9c2023-11-23T16:14:38ZengMDPI AGFoods2304-81582022-09-011118286910.3390/foods11182869Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous SuspensionXue Li0Zhifei He1Jingbing Xu2Chang Su3Xu Xiao4Ling Zhang5Huanhuan Zhang6Hongjun Li7College of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaChongqing Institute for Food and Drug Control, Chongqing 401121, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaAgricultural Product Processing Institute, Chongqing Academy of Agricultural Science, Chongqing 401329, ChinaAgricultural Product Processing Institute, Chongqing Academy of Agricultural Science, Chongqing 401329, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaAs a natural calcium resource, animal bone needs to be miniaturized to the nanoscale to improve palatability and absorption capacity. To explore the mechanism of high-pressure homogenization (HPH) in preparing natural bone aqueous nanosuspensions, the relationships between the changes in protein conformation, solubility and quality characteristics of rabbit bone aqueous suspensions (RBAS) prepared by different HPH cycles were studied. The results showed that the improvements in particle size, stability and calcium solubility of RBASs could be mainly attributed to the improvement of protein solubility induced by the changes in protein conformation. HPH treatment led to the denaturation and degradation of protein in rabbit bone, generating soluble peptides and improving the stability of the suspensions by enhancing the surface charge of the particles. When collagen as the main protein was partially degraded, the hydroxyapatite in the bone was crushed into tiny particles. The increase in the particle-specific surface area led to the release of calcium ions, which chelated with the peptides to produce peptide calcium. However, excessive HPH treatment caused the production of protein macromolecular aggregates and affected the quality of RBASs. This study is helpful to promote the application of HPH technology in animal bone nanoprocessing.https://www.mdpi.com/2304-8158/11/18/2869nanoprocessinghigh-pressure homogenization (HPH)rabbit bonecollagenpeptide calcium |
| spellingShingle | Xue Li Zhifei He Jingbing Xu Chang Su Xu Xiao Ling Zhang Huanhuan Zhang Hongjun Li Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension Foods nanoprocessing high-pressure homogenization (HPH) rabbit bone collagen peptide calcium |
| title | Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension |
| title_full | Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension |
| title_fullStr | Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension |
| title_full_unstemmed | Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension |
| title_short | Conformational Changes in Proteins Caused by High-Pressure Homogenization Promote Nanoparticle Formation in Natural Bone Aqueous Suspension |
| title_sort | conformational changes in proteins caused by high pressure homogenization promote nanoparticle formation in natural bone aqueous suspension |
| topic | nanoprocessing high-pressure homogenization (HPH) rabbit bone collagen peptide calcium |
| url | https://www.mdpi.com/2304-8158/11/18/2869 |
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