Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection
Opisthorchis viverrini is a carcinogenic parasite that can cause bile duct cancer called cholangiocarcinoma. A study of the immune response of this parasite in susceptible and non-susceptible hosts may provide a clue to develop vaccines and immunodiagnostic markers, which are currently not available...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2023-07-01
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| Series: | Parasitology |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S0031182023000112/type/journal_article |
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| author | Krongkarn Watakulsin Arpa Surapaitoon Lorina Handayani Ulag Sunheng Kaing Watcharapol Suyapoh Prasert Saichua Kanin Salao Sirikachorn Tangkawatana Sutas Suttiprapa |
| author_facet | Krongkarn Watakulsin Arpa Surapaitoon Lorina Handayani Ulag Sunheng Kaing Watcharapol Suyapoh Prasert Saichua Kanin Salao Sirikachorn Tangkawatana Sutas Suttiprapa |
| author_sort | Krongkarn Watakulsin |
| collection | DOAJ |
| description | Opisthorchis viverrini is a carcinogenic parasite that can cause bile duct cancer called cholangiocarcinoma. A study of the immune response of this parasite in susceptible and non-susceptible hosts may provide a clue to develop vaccines and immunodiagnostic markers, which are currently not available. Here, we compared the antibody response in susceptible Golden Syrian hamsters and non-susceptible BALB/c mice infected by the liver fluke. In mice, the antibody was detected between 1 and 2 weeks post-infection, whereas it was positive between 2 and 4 weeks post-infection in hamsters. Immunolocalization revealed that the antibody from mice reacts strongly with the tegumental surface and gut epithelium of the worm, while hamster antibody showed a weak signal in the tegument and a comparable signal in the gut of the worm. Immunoblot of the tegumental proteins demonstrated that while hamster antibody showed a broad specificity, mice strongly reacted with a single protein band. Mass spectrometry revealed these immunogenic targets. Recombinant proteins of the reactive targets were produced in the bacterial expression system. The immunoblot of these recombinant proteins confirm the reactivity of their native form. In summary, there is a different antibody response against O. viverrini infection in susceptible and non-susceptible hosts. The non-susceptible host reacts quicker and stronger than the susceptible host. |
| first_indexed | 2024-03-12T22:06:39Z |
| format | Article |
| id | doaj.art-fa218556ccea4ac4a5805563bc25726e |
| institution | Directory Open Access Journal |
| issn | 0031-1820 1469-8161 |
| language | English |
| last_indexed | 2024-03-12T22:06:39Z |
| publishDate | 2023-07-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Parasitology |
| spelling | doaj.art-fa218556ccea4ac4a5805563bc25726e2023-07-24T11:58:56ZengCambridge University PressParasitology0031-18201469-81612023-07-0115065366010.1017/S0031182023000112Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infectionKrongkarn Watakulsin0Arpa Surapaitoon1Lorina Handayani Ulag2Sunheng Kaing3Watcharapol Suyapoh4Prasert Saichua5Kanin Salao6Sirikachorn Tangkawatana7Sutas Suttiprapa8https://orcid.org/0000-0002-2323-2344Department of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jakarta 13210, IndonesiaDepartment of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandTropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandOpisthorchis viverrini is a carcinogenic parasite that can cause bile duct cancer called cholangiocarcinoma. A study of the immune response of this parasite in susceptible and non-susceptible hosts may provide a clue to develop vaccines and immunodiagnostic markers, which are currently not available. Here, we compared the antibody response in susceptible Golden Syrian hamsters and non-susceptible BALB/c mice infected by the liver fluke. In mice, the antibody was detected between 1 and 2 weeks post-infection, whereas it was positive between 2 and 4 weeks post-infection in hamsters. Immunolocalization revealed that the antibody from mice reacts strongly with the tegumental surface and gut epithelium of the worm, while hamster antibody showed a weak signal in the tegument and a comparable signal in the gut of the worm. Immunoblot of the tegumental proteins demonstrated that while hamster antibody showed a broad specificity, mice strongly reacted with a single protein band. Mass spectrometry revealed these immunogenic targets. Recombinant proteins of the reactive targets were produced in the bacterial expression system. The immunoblot of these recombinant proteins confirm the reactivity of their native form. In summary, there is a different antibody response against O. viverrini infection in susceptible and non-susceptible hosts. The non-susceptible host reacts quicker and stronger than the susceptible host.https://www.cambridge.org/core/product/identifier/S0031182023000112/type/journal_articleAntibodycarcinogenicliver flukenon-susceptibleOpisthorchis viverrinisusceptible |
| spellingShingle | Krongkarn Watakulsin Arpa Surapaitoon Lorina Handayani Ulag Sunheng Kaing Watcharapol Suyapoh Prasert Saichua Kanin Salao Sirikachorn Tangkawatana Sutas Suttiprapa Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection Parasitology Antibody carcinogenic liver fluke non-susceptible Opisthorchis viverrini susceptible |
| title | Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection |
| title_full | Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection |
| title_fullStr | Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection |
| title_full_unstemmed | Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection |
| title_short | Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection |
| title_sort | distinct antibody response in susceptible and non susceptible hosts of the carcinogenic liver fluke opisthorchis viverrini infection |
| topic | Antibody carcinogenic liver fluke non-susceptible Opisthorchis viverrini susceptible |
| url | https://www.cambridge.org/core/product/identifier/S0031182023000112/type/journal_article |
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