Two Multiplex PCR Methods for Detecting Several Pathogens Associated with Feline Respiratory and Intestinal Tracts
Respiratory tract and intestinal diseases are common threats to feline health. Coinfection with multiple pathogens is not rare among clinical infectious cases. Rapid diagnosis of these coinfections is of great significance for timely and effective clinical treatment. In this study, two novel multipl...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/1/14 |
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| author | Xiangyu Xiao Xiangqi Hao Bo Chen Pei Zhou Shoujun Li |
| author_facet | Xiangyu Xiao Xiangqi Hao Bo Chen Pei Zhou Shoujun Li |
| author_sort | Xiangyu Xiao |
| collection | DOAJ |
| description | Respiratory tract and intestinal diseases are common threats to feline health. Coinfection with multiple pathogens is not rare among clinical infectious cases. Rapid diagnosis of these coinfections is of great significance for timely and effective clinical treatment. In this study, two novel multiplex polymerase chain reactions (mPCRs) were established for simultaneous detection of four pathogens associated with the feline intestinal tract (feline coronavirus (FCoV), feline astrovirus (FeAstV), feline panleukopenia virus (FPV) and feline kobuvirus (FeKoV)) and five pathogens associated with the respiratory tract (feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), feline leukemia virus (FeLV), <i>Chlamydia felis</i> (<i>C. felis</i>) and influenza A virus (IAV)). The results of sensitivity analysis revealed that the detection limits for FeKoV, FPV, FeAstV, FCoV, IAV, <i>C. felis</i>, FeLV, FHV-1 and FCV were 10<sup>3</sup>, 10<sup>4</sup>, 10<sup>3</sup>, 10<sup>3</sup>, 10<sup>3</sup>, 10<sup>4</sup>, 10<sup>4</sup>, 10<sup>5</sup> and 10<sup>5</sup> copies/µL, respectively. Moreover, the specificity of the two mPCRs was high. When the two mPCRs were applied to clinical samples, the assay worked well. In conclusion, we established two mPCR methods that provide an excellent tool for the diagnosis and monitoring of pathogens associated with the feline respiratory and intestinal tracts. |
| first_indexed | 2024-03-09T11:03:45Z |
| format | Article |
| id | doaj.art-fa22f17725614647adb835b248224608 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T11:03:45Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-fa22f17725614647adb835b2482246082023-12-01T01:05:21ZengMDPI AGVeterinary Sciences2306-73812022-12-011011410.3390/vetsci10010014Two Multiplex PCR Methods for Detecting Several Pathogens Associated with Feline Respiratory and Intestinal TractsXiangyu Xiao0Xiangqi Hao1Bo Chen2Pei Zhou3Shoujun Li4Guangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaRespiratory tract and intestinal diseases are common threats to feline health. Coinfection with multiple pathogens is not rare among clinical infectious cases. Rapid diagnosis of these coinfections is of great significance for timely and effective clinical treatment. In this study, two novel multiplex polymerase chain reactions (mPCRs) were established for simultaneous detection of four pathogens associated with the feline intestinal tract (feline coronavirus (FCoV), feline astrovirus (FeAstV), feline panleukopenia virus (FPV) and feline kobuvirus (FeKoV)) and five pathogens associated with the respiratory tract (feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), feline leukemia virus (FeLV), <i>Chlamydia felis</i> (<i>C. felis</i>) and influenza A virus (IAV)). The results of sensitivity analysis revealed that the detection limits for FeKoV, FPV, FeAstV, FCoV, IAV, <i>C. felis</i>, FeLV, FHV-1 and FCV were 10<sup>3</sup>, 10<sup>4</sup>, 10<sup>3</sup>, 10<sup>3</sup>, 10<sup>3</sup>, 10<sup>4</sup>, 10<sup>4</sup>, 10<sup>5</sup> and 10<sup>5</sup> copies/µL, respectively. Moreover, the specificity of the two mPCRs was high. When the two mPCRs were applied to clinical samples, the assay worked well. In conclusion, we established two mPCR methods that provide an excellent tool for the diagnosis and monitoring of pathogens associated with the feline respiratory and intestinal tracts.https://www.mdpi.com/2306-7381/10/1/14multiplex PCRfeline pathogenssimultaneous detection |
| spellingShingle | Xiangyu Xiao Xiangqi Hao Bo Chen Pei Zhou Shoujun Li Two Multiplex PCR Methods for Detecting Several Pathogens Associated with Feline Respiratory and Intestinal Tracts Veterinary Sciences multiplex PCR feline pathogens simultaneous detection |
| title | Two Multiplex PCR Methods for Detecting Several Pathogens Associated with Feline Respiratory and Intestinal Tracts |
| title_full | Two Multiplex PCR Methods for Detecting Several Pathogens Associated with Feline Respiratory and Intestinal Tracts |
| title_fullStr | Two Multiplex PCR Methods for Detecting Several Pathogens Associated with Feline Respiratory and Intestinal Tracts |
| title_full_unstemmed | Two Multiplex PCR Methods for Detecting Several Pathogens Associated with Feline Respiratory and Intestinal Tracts |
| title_short | Two Multiplex PCR Methods for Detecting Several Pathogens Associated with Feline Respiratory and Intestinal Tracts |
| title_sort | two multiplex pcr methods for detecting several pathogens associated with feline respiratory and intestinal tracts |
| topic | multiplex PCR feline pathogens simultaneous detection |
| url | https://www.mdpi.com/2306-7381/10/1/14 |
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