Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates
Abstract Background Escherichia coli (E. coli) O25b/ST131 clone causes urinary tract infection (UTI) and is associated with a broad spectrum of other infections, such as intra-abdominal and soft tissue infections, that can be affecting bloodstream infections. Therefore, since O25b/ST131 has been rep...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12941-022-00526-2 |
| _version_ | 1828328059770503168 |
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| author | Amin Khoshbayan Rezvan Golmoradi Zadeh Majid Taati Moghadam Shiva Mirkalantari Atieh Darbandi |
| author_facet | Amin Khoshbayan Rezvan Golmoradi Zadeh Majid Taati Moghadam Shiva Mirkalantari Atieh Darbandi |
| author_sort | Amin Khoshbayan |
| collection | DOAJ |
| description | Abstract Background Escherichia coli (E. coli) O25b/ST131 clone causes urinary tract infection (UTI) and is associated with a broad spectrum of other infections, such as intra-abdominal and soft tissue infections, that can be affecting bloodstream infections. Therefore, since O25b/ST131 has been reported in several studies from Iran, in the current study, we have investigated the molecular characteristics, typing, and biofilm formation of O25b/ST131 clone type E. coli collected from UTI specimens. Methods A total of 173 E. coli isolates from UTI were collected. The susceptibility to all fourth generations of cephalosporins (cefazolin, cefuroxime, ceftriaxone, cefotaxime, ceftazidime, cefepime) and ampicillin, ampicillin-sulbactam and aztreonam was determined. Class A ESBLs, class D ESBL and the presence of pabB gene screenings to detect of O25b/ST131 clone type were performed by using of PCR. Biofilm formation was compared between O25b/ST131 isolates and non-O25b/ST131 isolates. Finally, ERIC-PCR was used for typing of ESBL positive isolates. Results Ninety-four ESBL positive were detected of which 79 of them were O25b/ST131. Antimicrobial susceptibility test data showed that most antibiotics had a higher rate of resistance in isolates of the O25b/ST131 clonal type. Biofilm formation showed that there was a weak association between O25b/ST131 clone type isolates and the level of the biofilm formation. ERIC-PCR results showed that E. coli isolates were genetically diverse and classified into 14 groups. Conclusion Our results demonstrated the importance and high prevalence of E. coli O25b/ST131 among UTI isolates with the ability to spread fast and disseminate antibiotic resistance genes. |
| first_indexed | 2024-04-13T20:04:39Z |
| format | Article |
| id | doaj.art-fa28df782b0e4e2585b7fd15f102b844 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-04-13T20:04:39Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-fa28df782b0e4e2585b7fd15f102b8442022-12-22T02:32:06ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112022-08-012111810.1186/s12941-022-00526-2Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolatesAmin Khoshbayan0Rezvan Golmoradi Zadeh1Majid Taati Moghadam2Shiva Mirkalantari3Atieh Darbandi4Department of Microbiology, School of Medicine, Iran University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesAbstract Background Escherichia coli (E. coli) O25b/ST131 clone causes urinary tract infection (UTI) and is associated with a broad spectrum of other infections, such as intra-abdominal and soft tissue infections, that can be affecting bloodstream infections. Therefore, since O25b/ST131 has been reported in several studies from Iran, in the current study, we have investigated the molecular characteristics, typing, and biofilm formation of O25b/ST131 clone type E. coli collected from UTI specimens. Methods A total of 173 E. coli isolates from UTI were collected. The susceptibility to all fourth generations of cephalosporins (cefazolin, cefuroxime, ceftriaxone, cefotaxime, ceftazidime, cefepime) and ampicillin, ampicillin-sulbactam and aztreonam was determined. Class A ESBLs, class D ESBL and the presence of pabB gene screenings to detect of O25b/ST131 clone type were performed by using of PCR. Biofilm formation was compared between O25b/ST131 isolates and non-O25b/ST131 isolates. Finally, ERIC-PCR was used for typing of ESBL positive isolates. Results Ninety-four ESBL positive were detected of which 79 of them were O25b/ST131. Antimicrobial susceptibility test data showed that most antibiotics had a higher rate of resistance in isolates of the O25b/ST131 clonal type. Biofilm formation showed that there was a weak association between O25b/ST131 clone type isolates and the level of the biofilm formation. ERIC-PCR results showed that E. coli isolates were genetically diverse and classified into 14 groups. Conclusion Our results demonstrated the importance and high prevalence of E. coli O25b/ST131 among UTI isolates with the ability to spread fast and disseminate antibiotic resistance genes.https://doi.org/10.1186/s12941-022-00526-2Escherichia coliST131ESBLO25b/ST131Iran |
| spellingShingle | Amin Khoshbayan Rezvan Golmoradi Zadeh Majid Taati Moghadam Shiva Mirkalantari Atieh Darbandi Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates Annals of Clinical Microbiology and Antimicrobials Escherichia coli ST131 ESBL O25b/ST131 Iran |
| title | Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates |
| title_full | Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates |
| title_fullStr | Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates |
| title_full_unstemmed | Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates |
| title_short | Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates |
| title_sort | molecular determination of o25b st131 clone type among extended spectrum β lactamases production escherichia coli recovering from urinary tract infection isolates |
| topic | Escherichia coli ST131 ESBL O25b/ST131 Iran |
| url | https://doi.org/10.1186/s12941-022-00526-2 |
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