Short-Term Variations of C18:1 <i>Trans</i> Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of Oils
The objective of this study was to evaluate short-term variations of <i>trans</i> fatty acids (TFA) in plasma lipoproteins and ruminal fermentation parameters of non-lactating cows subjected to ruminal pulses of vegetable oils. Three non-lactating, non-pregnant Holstein cows, each with a...
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MDPI AG
2021-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/3/788 |
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| author | Einar Vargas-Bello-Pérez Juan J. Loor Philip C. Garnsworthy |
| author_facet | Einar Vargas-Bello-Pérez Juan J. Loor Philip C. Garnsworthy |
| author_sort | Einar Vargas-Bello-Pérez |
| collection | DOAJ |
| description | The objective of this study was to evaluate short-term variations of <i>trans</i> fatty acids (TFA) in plasma lipoproteins and ruminal fermentation parameters of non-lactating cows subjected to ruminal pulses of vegetable oils. Three non-lactating, non-pregnant Holstein cows, each with a ruminal cannula, were arranged in a 3 × 3 Latin square design with three-day pulsing periods and four-day washout intervals between treatments. Cows were treated with single ruminal pulses of: (1) control (skimmed milk (SM); 500 mL); (2) soybean oil (SO; 250 g/d in 500 mL of SM) and (3) partially-hydrogenated vegetable oil (PHVO; 250 g/d in 500 mL of SM). Time changes after infusion in TFA contents were only observed for plasma C18:1 <i>trans</i>-4, <i>trans</i>-5 and <i>trans</i>-12, and high-density lipoprotein fraction C18:1 <i>trans</i>-9. After ruminal pulses, concentration of acetate decreased linearly; molar concentrations of propionate and valerate increased linearly; molar concentrations of butyrate and isovalerate changed quadratically and were greater at 1 h than at other times. There was an accumulation of several C18:1 TFA in plasma and lipoproteins, especially on the third day of pulsing. Overall, naturally occurring C18:1 TFA isomers (produced during ruminal biohydrogenation of SO) and preformed TFA (supplied by PHVO) elicited differential TFA partitioning and transport in plasma and lipoproteins. |
| first_indexed | 2024-03-10T13:19:54Z |
| format | Article |
| id | doaj.art-fa2a3d20cc1745fb8f97eac465dd418e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:19:54Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-fa2a3d20cc1745fb8f97eac465dd418e2023-11-21T10:10:33ZengMDPI AGAnimals2076-26152021-03-0111378810.3390/ani11030788Short-Term Variations of C18:1 <i>Trans</i> Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of OilsEinar Vargas-Bello-Pérez0Juan J. Loor1Philip C. Garnsworthy2Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 3, DK-1870 Frederiksberg C, DenmarkDepartment of Animal Sciences and Division of Nutritional Sciences, University of Illinois, 1207 West Gregory Drive, Urbana, IL 61801, USASchool of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough LE12 5RD, UKThe objective of this study was to evaluate short-term variations of <i>trans</i> fatty acids (TFA) in plasma lipoproteins and ruminal fermentation parameters of non-lactating cows subjected to ruminal pulses of vegetable oils. Three non-lactating, non-pregnant Holstein cows, each with a ruminal cannula, were arranged in a 3 × 3 Latin square design with three-day pulsing periods and four-day washout intervals between treatments. Cows were treated with single ruminal pulses of: (1) control (skimmed milk (SM); 500 mL); (2) soybean oil (SO; 250 g/d in 500 mL of SM) and (3) partially-hydrogenated vegetable oil (PHVO; 250 g/d in 500 mL of SM). Time changes after infusion in TFA contents were only observed for plasma C18:1 <i>trans</i>-4, <i>trans</i>-5 and <i>trans</i>-12, and high-density lipoprotein fraction C18:1 <i>trans</i>-9. After ruminal pulses, concentration of acetate decreased linearly; molar concentrations of propionate and valerate increased linearly; molar concentrations of butyrate and isovalerate changed quadratically and were greater at 1 h than at other times. There was an accumulation of several C18:1 TFA in plasma and lipoproteins, especially on the third day of pulsing. Overall, naturally occurring C18:1 TFA isomers (produced during ruminal biohydrogenation of SO) and preformed TFA (supplied by PHVO) elicited differential TFA partitioning and transport in plasma and lipoproteins.https://www.mdpi.com/2076-2615/11/3/788biohydrogenationcowslipoproteinsruminal fluid<i>trans</i> fatty acidsvaccenic acid |
| spellingShingle | Einar Vargas-Bello-Pérez Juan J. Loor Philip C. Garnsworthy Short-Term Variations of C18:1 <i>Trans</i> Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of Oils Animals biohydrogenation cows lipoproteins ruminal fluid <i>trans</i> fatty acids vaccenic acid |
| title | Short-Term Variations of C18:1 <i>Trans</i> Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of Oils |
| title_full | Short-Term Variations of C18:1 <i>Trans</i> Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of Oils |
| title_fullStr | Short-Term Variations of C18:1 <i>Trans</i> Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of Oils |
| title_full_unstemmed | Short-Term Variations of C18:1 <i>Trans</i> Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of Oils |
| title_short | Short-Term Variations of C18:1 <i>Trans</i> Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of Oils |
| title_sort | short term variations of c18 1 i trans i fatty acids in plasma lipoproteins and ruminal fermentation parameters of non lactating cows subjected to ruminal pulses of oils |
| topic | biohydrogenation cows lipoproteins ruminal fluid <i>trans</i> fatty acids vaccenic acid |
| url | https://www.mdpi.com/2076-2615/11/3/788 |
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