Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation
The present study investigated the anti-microbial and anti-mycotoxigenic activities of the ethyl acetate extract (EA) and a bioactive compound obtained from an endophytic fungus Alternaria alternata isolated from Catharanthus roseus leaves. A. alternata was identified using PCR-based 5.8S rDNA seque...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Mycology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21501203.2018.1541933 |
| _version_ | 1818154234200195072 |
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| author | T. N. Sudharshana H. N. Venkatesh Borah Nayana K. Manjunath D. C. Mohana |
| author_facet | T. N. Sudharshana H. N. Venkatesh Borah Nayana K. Manjunath D. C. Mohana |
| author_sort | T. N. Sudharshana |
| collection | DOAJ |
| description | The present study investigated the anti-microbial and anti-mycotoxigenic activities of the ethyl acetate extract (EA) and a bioactive compound obtained from an endophytic fungus Alternaria alternata isolated from Catharanthus roseus leaves. A. alternata was identified using PCR-based 5.8S rDNA sequencing. The EA and bioactive compound, p-Coumaric acid (PC), showed concentration-dependent broad-spectrum anti-microbial activity against the tested bacteria, yeast, and fungi with MICs ranging from 7.8 to 250 µg/mL. The in vitro production of aflatoxin B1 (AFB1) from Aspergillus flavus and fumonisin B1 (FB1) from Fusarium verticillioides was completely inhibited by EA and PC at 400 µg/mL. The synthesis of the membrane-bound ergosterol from A. flavus and F. verticillioides was strongly inhibited by PC at 200 µg/mL. The EA and PC were found to show significant anti-microbial and anti-mycotoxigenic activities, hence, they could be explored as protective agents for preventing microbial deterioration and mycotoxins accumulation in food and feedstuffs during pre- and post-harvest and storage. |
| first_indexed | 2024-12-11T14:23:16Z |
| format | Article |
| id | doaj.art-fa2b7450e62e4e9bb91b4fafa697c957 |
| institution | Directory Open Access Journal |
| issn | 2150-1203 2150-1211 |
| language | English |
| last_indexed | 2024-12-11T14:23:16Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj.art-fa2b7450e62e4e9bb91b4fafa697c9572022-12-22T01:02:49ZengTaylor & Francis GroupMycology2150-12032150-12112019-01-01101404810.1080/21501203.2018.15419331541933Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolationT. N. Sudharshana0H. N. Venkatesh1Borah Nayana2K. Manjunath3D. C. Mohana4Bangalore UniversityBangalore UniversityBangalore UniversityBangalore UniversityBangalore UniversityThe present study investigated the anti-microbial and anti-mycotoxigenic activities of the ethyl acetate extract (EA) and a bioactive compound obtained from an endophytic fungus Alternaria alternata isolated from Catharanthus roseus leaves. A. alternata was identified using PCR-based 5.8S rDNA sequencing. The EA and bioactive compound, p-Coumaric acid (PC), showed concentration-dependent broad-spectrum anti-microbial activity against the tested bacteria, yeast, and fungi with MICs ranging from 7.8 to 250 µg/mL. The in vitro production of aflatoxin B1 (AFB1) from Aspergillus flavus and fumonisin B1 (FB1) from Fusarium verticillioides was completely inhibited by EA and PC at 400 µg/mL. The synthesis of the membrane-bound ergosterol from A. flavus and F. verticillioides was strongly inhibited by PC at 200 µg/mL. The EA and PC were found to show significant anti-microbial and anti-mycotoxigenic activities, hence, they could be explored as protective agents for preventing microbial deterioration and mycotoxins accumulation in food and feedstuffs during pre- and post-harvest and storage.http://dx.doi.org/10.1080/21501203.2018.1541933Endophytic Alternaria alternataethyl acetate extractp-Coumaric acidanti-microbial activityaflatoxin B1fumonisin B1 |
| spellingShingle | T. N. Sudharshana H. N. Venkatesh Borah Nayana K. Manjunath D. C. Mohana Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation Mycology Endophytic Alternaria alternata ethyl acetate extract p-Coumaric acid anti-microbial activity aflatoxin B1 fumonisin B1 |
| title | Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation |
| title_full | Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation |
| title_fullStr | Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation |
| title_full_unstemmed | Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation |
| title_short | Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation |
| title_sort | anti microbial and anti mycotoxigenic activities of endophytic alternaria alternata isolated from catharanthus roseus l g don molecular characterisation and bioactive compound isolation |
| topic | Endophytic Alternaria alternata ethyl acetate extract p-Coumaric acid anti-microbial activity aflatoxin B1 fumonisin B1 |
| url | http://dx.doi.org/10.1080/21501203.2018.1541933 |
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