The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures
The roach Eurycotis floridana was reared on a semisynthetic diet containing minimal cholesterol-4-14C (0.005%) supplemented with cholestanol-7α-3H (0.1%), and various tissues of the insect were separated into ``nuclear,'' ``mitochondrial,'' and ``microsomal'' fractions...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1966-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520389690 |
| _version_ | 1818941237057552384 |
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| author | N.L. Lasser R.B. Clayton |
| author_facet | N.L. Lasser R.B. Clayton |
| author_sort | N.L. Lasser |
| collection | DOAJ |
| description | The roach Eurycotis floridana was reared on a semisynthetic diet containing minimal cholesterol-4-14C (0.005%) supplemented with cholestanol-7α-3H (0.1%), and various tissues of the insect were separated into ``nuclear,'' ``mitochondrial,'' and ``microsomal'' fractions by differential centrifugation. Muscle and salivary gland were satisfactorily fractionated and results from these are reported together with some, as yet, incomplete results for nerve.The major concentrations of unesterified sterol and lipid phosphorus were in the mitochondrial and microsomal fractions.The unesterified sterols of the subcellular fractions were separated by alumina and gas-liquid chromatography. The emergent peaks corresponding to cholesterol, cholestanol, and Δ7-cholestenol (a metabolite of cholestanol) were assayed for 3H and 14C. No sterol was confined to any one subcellular fraction. The proportions of the three sterols were closely similar in all membranous components of the same tissue, but were different in different tissues.It is suggested that the different subcellular membranes of a given tissue contain similar repeating structural units in which the individual sterols occupy sterically characteristic spaces. |
| first_indexed | 2024-12-20T06:52:20Z |
| format | Article |
| id | doaj.art-fa2e2fa2877d49849f7d853d7416692b |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T06:52:20Z |
| publishDate | 1966-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-fa2e2fa2877d49849f7d853d7416692b2022-12-21T19:49:30ZengElsevierJournal of Lipid Research0022-22751966-05-0173413421The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structuresN.L. Lasser0R.B. Clayton1Department of Chemistry, Harvard University, Cambridge, Massachusetts; Department of Psychiatry, Stanford University School of Medicine, Palo Alto, CaliforniaDepartment of Chemistry, Harvard University, Cambridge, Massachusetts; Department of Psychiatry, Stanford University School of Medicine, Palo Alto, CaliforniaThe roach Eurycotis floridana was reared on a semisynthetic diet containing minimal cholesterol-4-14C (0.005%) supplemented with cholestanol-7α-3H (0.1%), and various tissues of the insect were separated into ``nuclear,'' ``mitochondrial,'' and ``microsomal'' fractions by differential centrifugation. Muscle and salivary gland were satisfactorily fractionated and results from these are reported together with some, as yet, incomplete results for nerve.The major concentrations of unesterified sterol and lipid phosphorus were in the mitochondrial and microsomal fractions.The unesterified sterols of the subcellular fractions were separated by alumina and gas-liquid chromatography. The emergent peaks corresponding to cholesterol, cholestanol, and Δ7-cholestenol (a metabolite of cholestanol) were assayed for 3H and 14C. No sterol was confined to any one subcellular fraction. The proportions of the three sterols were closely similar in all membranous components of the same tissue, but were different in different tissues.It is suggested that the different subcellular membranes of a given tissue contain similar repeating structural units in which the individual sterols occupy sterically characteristic spaces.http://www.sciencedirect.com/science/article/pii/S0022227520389690roachmusclesalivary glandnervefractionationsterols |
| spellingShingle | N.L. Lasser R.B. Clayton The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures Journal of Lipid Research roach muscle salivary gland nerve fractionation sterols |
| title | The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures |
| title_full | The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures |
| title_fullStr | The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures |
| title_full_unstemmed | The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures |
| title_short | The intracellular distribution of sterols in Eurycotis floridana and its possible relation to subcellular membrane structures |
| title_sort | intracellular distribution of sterols in eurycotis floridana and its possible relation to subcellular membrane structures |
| topic | roach muscle salivary gland nerve fractionation sterols |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520389690 |
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