Biochar Amendment Suppressed <i>Fusarium</i> Wilt and Altered the Rhizosphere Microbial Composition of Tomatoes
The effectiveness of biochar application to promote plant growth and suppress plant diseases is usually dependent on the application dose of the biochar. Here, we evaluated the effects of biochar supplied at 0%, 1%, 2%, and 3% (<i>w</i>/<i>w</i>) on tomato growth, <i>Fu...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/13/7/1811 |
| _version_ | 1797590689152761856 |
|---|---|
| author | Xue Jin Xingang Zhou Fengzhi Wu Wensheng Xiang Kai Pan |
| author_facet | Xue Jin Xingang Zhou Fengzhi Wu Wensheng Xiang Kai Pan |
| author_sort | Xue Jin |
| collection | DOAJ |
| description | The effectiveness of biochar application to promote plant growth and suppress plant diseases is usually dependent on the application dose of the biochar. Here, we evaluated the effects of biochar supplied at 0%, 1%, 2%, and 3% (<i>w</i>/<i>w</i>) on tomato growth, <i>Fusarium</i> wilt disease severity, and rhizosphere microbial community diversity. We found that biochar applied at 1% and 2% promoted tomato growth and decreased the severity of <i>Fusarium</i> wilt disease. High-throughput amplicon sequencing indicated that 1% biochar decreased the alpha diversity and altered the composition of the bacterial and fungal community in the tomato rhizosphere, increasing the abundance of potential plant-beneficial microorganisms. Quantitative PCR confirmed that all doses of biochar increased the abundance of rhizosphere bacteria; biochar applied at 1% and 2% decreased the abundance of rhizosphere fungi and <i>Fusarium oxysporum f.</i> sp. <i>Lycopersici</i> (FOL), while biochar applied at 3% increased abundance of FOL. Our results indicated that biochar applied at 1% and 2% suppressed tomato <i>Fusarium</i> wilt disease, which might be linked to the change of the rhizosphere microbial community structure and increased the abundance of potential plant-beneficial microorganisms such as <i>Pseudomonas</i> sp. within the microbiome. |
| first_indexed | 2024-03-11T01:24:06Z |
| format | Article |
| id | doaj.art-fa2e90037d264907ade5683bc1a5ea5a |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T01:24:06Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-fa2e90037d264907ade5683bc1a5ea5a2023-11-18T17:56:32ZengMDPI AGAgronomy2073-43952023-07-01137181110.3390/agronomy13071811Biochar Amendment Suppressed <i>Fusarium</i> Wilt and Altered the Rhizosphere Microbial Composition of TomatoesXue Jin0Xingang Zhou1Fengzhi Wu2Wensheng Xiang3Kai Pan4Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture, Northeast Agricultural University, Harbin 150030, ChinaThe effectiveness of biochar application to promote plant growth and suppress plant diseases is usually dependent on the application dose of the biochar. Here, we evaluated the effects of biochar supplied at 0%, 1%, 2%, and 3% (<i>w</i>/<i>w</i>) on tomato growth, <i>Fusarium</i> wilt disease severity, and rhizosphere microbial community diversity. We found that biochar applied at 1% and 2% promoted tomato growth and decreased the severity of <i>Fusarium</i> wilt disease. High-throughput amplicon sequencing indicated that 1% biochar decreased the alpha diversity and altered the composition of the bacterial and fungal community in the tomato rhizosphere, increasing the abundance of potential plant-beneficial microorganisms. Quantitative PCR confirmed that all doses of biochar increased the abundance of rhizosphere bacteria; biochar applied at 1% and 2% decreased the abundance of rhizosphere fungi and <i>Fusarium oxysporum f.</i> sp. <i>Lycopersici</i> (FOL), while biochar applied at 3% increased abundance of FOL. Our results indicated that biochar applied at 1% and 2% suppressed tomato <i>Fusarium</i> wilt disease, which might be linked to the change of the rhizosphere microbial community structure and increased the abundance of potential plant-beneficial microorganisms such as <i>Pseudomonas</i> sp. within the microbiome.https://www.mdpi.com/2073-4395/13/7/1811tomatobiochar<i>Fusarium</i> wiltrhizosphere microbial communities |
| spellingShingle | Xue Jin Xingang Zhou Fengzhi Wu Wensheng Xiang Kai Pan Biochar Amendment Suppressed <i>Fusarium</i> Wilt and Altered the Rhizosphere Microbial Composition of Tomatoes Agronomy tomato biochar <i>Fusarium</i> wilt rhizosphere microbial communities |
| title | Biochar Amendment Suppressed <i>Fusarium</i> Wilt and Altered the Rhizosphere Microbial Composition of Tomatoes |
| title_full | Biochar Amendment Suppressed <i>Fusarium</i> Wilt and Altered the Rhizosphere Microbial Composition of Tomatoes |
| title_fullStr | Biochar Amendment Suppressed <i>Fusarium</i> Wilt and Altered the Rhizosphere Microbial Composition of Tomatoes |
| title_full_unstemmed | Biochar Amendment Suppressed <i>Fusarium</i> Wilt and Altered the Rhizosphere Microbial Composition of Tomatoes |
| title_short | Biochar Amendment Suppressed <i>Fusarium</i> Wilt and Altered the Rhizosphere Microbial Composition of Tomatoes |
| title_sort | biochar amendment suppressed i fusarium i wilt and altered the rhizosphere microbial composition of tomatoes |
| topic | tomato biochar <i>Fusarium</i> wilt rhizosphere microbial communities |
| url | https://www.mdpi.com/2073-4395/13/7/1811 |
| work_keys_str_mv | AT xuejin biocharamendmentsuppressedifusariumiwiltandalteredtherhizospheremicrobialcompositionoftomatoes AT xingangzhou biocharamendmentsuppressedifusariumiwiltandalteredtherhizospheremicrobialcompositionoftomatoes AT fengzhiwu biocharamendmentsuppressedifusariumiwiltandalteredtherhizospheremicrobialcompositionoftomatoes AT wenshengxiang biocharamendmentsuppressedifusariumiwiltandalteredtherhizospheremicrobialcompositionoftomatoes AT kaipan biocharamendmentsuppressedifusariumiwiltandalteredtherhizospheremicrobialcompositionoftomatoes |