Safety Evaluation of Tricalcium Phosphate/Collagen Nanocomposite Scaffold in Bone Defect in New Zealand White Rabbit Model
Objectives: Bone loss with skeletal trauma or metabolic diseases usually will require a bone graft. In addition, medical devices used for replacement in tissues such as bones and cartilages for more than 30 days must be checked and controlled for biological safety. Materials and Methods: New Zealand...
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| Format: | Article |
| Language: | English |
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Aras Part Medical International Press
2019-10-01
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| Series: | Crescent Journal of Medical and Biological Sciences |
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| Online Access: | http://www.cjmb.org/pdf.php?id=401 |
| _version_ | 1819294694338723840 |
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| author | Hanie Farahi Seeyamak Mashhady Rafie Alireza Jahandideh Ahmad Asghari Seyed Hamed Shirazi-Beheshtiha |
| author_facet | Hanie Farahi Seeyamak Mashhady Rafie Alireza Jahandideh Ahmad Asghari Seyed Hamed Shirazi-Beheshtiha |
| author_sort | Hanie Farahi |
| collection | DOAJ |
| description | Objectives: Bone loss with skeletal trauma or metabolic diseases usually will require a bone graft. In addition, medical devices used for replacement in tissues such as bones and cartilages for more than 30 days must be checked and controlled for biological safety. Materials and Methods: New Zealand white rabbits were divided into two groups. The first group had no defects and was selected as the control group. In the experimental group, tricalcium phosphate/collagen (TCP/collagen) nanocomposite was utilized as the replacement tissue in the femoral defect site. Then, the factors of kidney, liver, and TCP/collagen biocompatibility were evaluated drawing on hematological quality. Free radicals are generated by the damaged tissue when there is a fracture in a bone. Oxidative stress is involved in this mechanism which is defined as the excessive imbalance of reactive oxygen species (ROS) and inappropriate antioxidant anti-mechanical mechanisms. Results: In the treatment group, malondialdehyde (MDA) level increased postoperatively in the 15th and 30th days, but in due course, it reduced on days 45 and 60. Further, glutathione peroxidase (GPX) enzyme increased after the surgery on days 15 and 30 in the test group and superoxide dismutase (SOD) enzyme demonstrated a slight increase in 15th day. The hematologic investigations were all within a normal limit, including hepatic enzymes, alanine transaminase, aspartate aminotransferase, and alkaline phosphatase (ALP), which indicate the liver damage, as well as creatinine and urea levels displaying the renal function. Conclusions: Overall, the results of the current study revealed that the oxidative stress factor in the treatment group was not higher compared to the control group, thus showing good biocompatibility of TCP/collagen nanocomposite. |
| first_indexed | 2024-12-24T04:30:23Z |
| format | Article |
| id | doaj.art-fa319968a44840a3b6699ecf75b0b33b |
| institution | Directory Open Access Journal |
| issn | 2148-9696 |
| language | English |
| last_indexed | 2024-12-24T04:30:23Z |
| publishDate | 2019-10-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-fa319968a44840a3b6699ecf75b0b33b2022-12-21T17:15:28ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962019-10-0164449454cjmb-2049Safety Evaluation of Tricalcium Phosphate/Collagen Nanocomposite Scaffold in Bone Defect in New Zealand White Rabbit ModelHanie Farahi0Seeyamak Mashhady Rafie1Alireza Jahandideh2Ahmad Asghari3Seyed Hamed Shirazi-Beheshtiha4Department of Small Animal Internal Medicine, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Small Animal Internal Medicine, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Veterinary Surgery, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Veterinary Surgery, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.Objectives: Bone loss with skeletal trauma or metabolic diseases usually will require a bone graft. In addition, medical devices used for replacement in tissues such as bones and cartilages for more than 30 days must be checked and controlled for biological safety. Materials and Methods: New Zealand white rabbits were divided into two groups. The first group had no defects and was selected as the control group. In the experimental group, tricalcium phosphate/collagen (TCP/collagen) nanocomposite was utilized as the replacement tissue in the femoral defect site. Then, the factors of kidney, liver, and TCP/collagen biocompatibility were evaluated drawing on hematological quality. Free radicals are generated by the damaged tissue when there is a fracture in a bone. Oxidative stress is involved in this mechanism which is defined as the excessive imbalance of reactive oxygen species (ROS) and inappropriate antioxidant anti-mechanical mechanisms. Results: In the treatment group, malondialdehyde (MDA) level increased postoperatively in the 15th and 30th days, but in due course, it reduced on days 45 and 60. Further, glutathione peroxidase (GPX) enzyme increased after the surgery on days 15 and 30 in the test group and superoxide dismutase (SOD) enzyme demonstrated a slight increase in 15th day. The hematologic investigations were all within a normal limit, including hepatic enzymes, alanine transaminase, aspartate aminotransferase, and alkaline phosphatase (ALP), which indicate the liver damage, as well as creatinine and urea levels displaying the renal function. Conclusions: Overall, the results of the current study revealed that the oxidative stress factor in the treatment group was not higher compared to the control group, thus showing good biocompatibility of TCP/collagen nanocomposite.http://www.cjmb.org/pdf.php?id=401tricalcium phosphate/collagennanocompositebiocompatibilityoxidative stressbone defect |
| spellingShingle | Hanie Farahi Seeyamak Mashhady Rafie Alireza Jahandideh Ahmad Asghari Seyed Hamed Shirazi-Beheshtiha Safety Evaluation of Tricalcium Phosphate/Collagen Nanocomposite Scaffold in Bone Defect in New Zealand White Rabbit Model Crescent Journal of Medical and Biological Sciences tricalcium phosphate/collagen nanocomposite biocompatibility oxidative stress bone defect |
| title | Safety Evaluation of Tricalcium Phosphate/Collagen Nanocomposite Scaffold in Bone Defect in New Zealand White Rabbit Model |
| title_full | Safety Evaluation of Tricalcium Phosphate/Collagen Nanocomposite Scaffold in Bone Defect in New Zealand White Rabbit Model |
| title_fullStr | Safety Evaluation of Tricalcium Phosphate/Collagen Nanocomposite Scaffold in Bone Defect in New Zealand White Rabbit Model |
| title_full_unstemmed | Safety Evaluation of Tricalcium Phosphate/Collagen Nanocomposite Scaffold in Bone Defect in New Zealand White Rabbit Model |
| title_short | Safety Evaluation of Tricalcium Phosphate/Collagen Nanocomposite Scaffold in Bone Defect in New Zealand White Rabbit Model |
| title_sort | safety evaluation of tricalcium phosphate collagen nanocomposite scaffold in bone defect in new zealand white rabbit model |
| topic | tricalcium phosphate/collagen nanocomposite biocompatibility oxidative stress bone defect |
| url | http://www.cjmb.org/pdf.php?id=401 |
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