High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
Lemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-01744-8 |
| _version_ | 1818993651827605504 |
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| author | Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi |
| author_facet | Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi |
| author_sort | Mariana Lemos Duarte |
| collection | DOAJ |
| description | Lemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful in understanding the spatio-temporal dynamics of opioid signaling. |
| first_indexed | 2024-12-20T20:45:27Z |
| format | Article |
| id | doaj.art-fa3462494d824628b60f24925ecc50e1 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-20T20:45:27Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-fa3462494d824628b60f24925ecc50e12022-12-21T19:27:03ZengNature PortfolioCommunications Biology2399-36422021-02-014111310.1038/s42003-021-01744-8High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamicsMariana Lemos Duarte0Nikita A. Trimbake1Achla Gupta2Christine Tumanut3Xiaomin Fan4Catherine Woods5Akila Ram6Ivone Gomes7Erin N. Bobeck8Deborah Schechtman9Lakshmi A. Devi10Department of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiAvantGen Inc.AvantGen Inc.AvantGen Inc.Department of Biology, Utah State UniversityDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiDepartment of Biology, Utah State UniversityDepartment of Biochemistry, University of São PauloDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount SinaiLemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful in understanding the spatio-temporal dynamics of opioid signaling.https://doi.org/10.1038/s42003-021-01744-8 |
| spellingShingle | Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics Communications Biology |
| title | High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics |
| title_full | High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics |
| title_fullStr | High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics |
| title_full_unstemmed | High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics |
| title_short | High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics |
| title_sort | high throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics |
| url | https://doi.org/10.1038/s42003-021-01744-8 |
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