The tymbal muscle of cicada has flight muscle-type sarcomeric architecture and protein expression
Abstract Background The structural and biochemical features of the tymbal (sound-producing) muscle of cicadas were studied by X-ray diffraction and immunochemistry, and compared with those of flight muscles from the same species. Results The X-ray diffraction pattern of the tymbal muscle was very si...
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Zoological Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40851-017-0077-4 |
| _version_ | 1818313327902720000 |
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| author | Hiroyuki Iwamoto |
| author_facet | Hiroyuki Iwamoto |
| author_sort | Hiroyuki Iwamoto |
| collection | DOAJ |
| description | Abstract Background The structural and biochemical features of the tymbal (sound-producing) muscle of cicadas were studied by X-ray diffraction and immunochemistry, and compared with those of flight muscles from the same species. Results The X-ray diffraction pattern of the tymbal muscle was very similar to that of the dorsal longitudinal flight muscle: In both muscles, the 2,0 equatorial reflection is much more intense than the 1,1, indicating that both muscles have a flight muscle-type myofilament lattice. In rigor, the first myosin/actin layer line reflection was finely lattice-sampled, indicating that the contractile proteins are arranged with a crystalline regularity as in asynchronous flight muscles. In contrast, the diffraction pattern from the tensor muscle, which modulates the sound by stressing the tymbal, did not show signs of such high regularity or flight muscle-type filament lattice. Electrophoretic patterns of myofibrillar proteins were also very similar in the tymbal muscle and flight muscles, but distinct from those from the tensor or leg muscles. The antibody raised against the flight muscle-specific troponin-I isoform reacted with an 80-kDa band from both tymbal and flight muscles, but with none of the bands from the tensor or leg muscles. Conclusion The close similarities of the structural and biochemical profiles between the tymbal and the flight muscles suggest the possibility that a set of flight muscle-specific proteins is diverted to the tymbal muscle to meet its demand for fast, repetitive contractions. |
| first_indexed | 2024-12-13T08:31:59Z |
| format | Article |
| id | doaj.art-fa36a068046e48c5852d371809c22257 |
| institution | Directory Open Access Journal |
| issn | 2056-306X |
| language | English |
| last_indexed | 2024-12-13T08:31:59Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Zoological Letters |
| spelling | doaj.art-fa36a068046e48c5852d371809c222572022-12-21T23:53:44ZengBMCZoological Letters2056-306X2017-09-013111010.1186/s40851-017-0077-4The tymbal muscle of cicada has flight muscle-type sarcomeric architecture and protein expressionHiroyuki Iwamoto0Japan Synchrotron Radiation Research InstituteAbstract Background The structural and biochemical features of the tymbal (sound-producing) muscle of cicadas were studied by X-ray diffraction and immunochemistry, and compared with those of flight muscles from the same species. Results The X-ray diffraction pattern of the tymbal muscle was very similar to that of the dorsal longitudinal flight muscle: In both muscles, the 2,0 equatorial reflection is much more intense than the 1,1, indicating that both muscles have a flight muscle-type myofilament lattice. In rigor, the first myosin/actin layer line reflection was finely lattice-sampled, indicating that the contractile proteins are arranged with a crystalline regularity as in asynchronous flight muscles. In contrast, the diffraction pattern from the tensor muscle, which modulates the sound by stressing the tymbal, did not show signs of such high regularity or flight muscle-type filament lattice. Electrophoretic patterns of myofibrillar proteins were also very similar in the tymbal muscle and flight muscles, but distinct from those from the tensor or leg muscles. The antibody raised against the flight muscle-specific troponin-I isoform reacted with an 80-kDa band from both tymbal and flight muscles, but with none of the bands from the tensor or leg muscles. Conclusion The close similarities of the structural and biochemical profiles between the tymbal and the flight muscles suggest the possibility that a set of flight muscle-specific proteins is diverted to the tymbal muscle to meet its demand for fast, repetitive contractions.http://link.springer.com/article/10.1186/s40851-017-0077-4Tymbal muscleCicadaInsect flight muscleTroponinSynchrotron radiationX-ray diffraction |
| spellingShingle | Hiroyuki Iwamoto The tymbal muscle of cicada has flight muscle-type sarcomeric architecture and protein expression Zoological Letters Tymbal muscle Cicada Insect flight muscle Troponin Synchrotron radiation X-ray diffraction |
| title | The tymbal muscle of cicada has flight muscle-type sarcomeric architecture and protein expression |
| title_full | The tymbal muscle of cicada has flight muscle-type sarcomeric architecture and protein expression |
| title_fullStr | The tymbal muscle of cicada has flight muscle-type sarcomeric architecture and protein expression |
| title_full_unstemmed | The tymbal muscle of cicada has flight muscle-type sarcomeric architecture and protein expression |
| title_short | The tymbal muscle of cicada has flight muscle-type sarcomeric architecture and protein expression |
| title_sort | tymbal muscle of cicada has flight muscle type sarcomeric architecture and protein expression |
| topic | Tymbal muscle Cicada Insect flight muscle Troponin Synchrotron radiation X-ray diffraction |
| url | http://link.springer.com/article/10.1186/s40851-017-0077-4 |
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