Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity
Recent advances in nanotechnology techniques enable the production of polymeric nanoparticles with specific morphologies and dimensions and, by tailoring their surfaces, one can manipulate their characteristics to suit specific applications. In this work we report an innovative approach for the immo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/11/6/744 |
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| author | Laura Chronopoulou Viviana Couto Sayalero Hassan Rahimi Aurelia Rughetti Cleofe Palocci |
| author_facet | Laura Chronopoulou Viviana Couto Sayalero Hassan Rahimi Aurelia Rughetti Cleofe Palocci |
| author_sort | Laura Chronopoulou |
| collection | DOAJ |
| description | Recent advances in nanotechnology techniques enable the production of polymeric nanoparticles with specific morphologies and dimensions and, by tailoring their surfaces, one can manipulate their characteristics to suit specific applications. In this work we report an innovative approach for the immobilization of a commercial lipase from <i>Candida rugosa</i> (CRL) which employs nanostructured polymeric carriers conjugated with anti-lipase monoclonal antibodies (MoAbs). MoAbs were chemically conjugated on the surface of polymeric nanoparticles and used to selectively adsorb CRL molecules. Hydrolytic enzymatic assays evidenced that such immobilization technique afforded a significant enhancement of enzymatic activity in comparison to the free enzyme. |
| first_indexed | 2024-03-10T10:19:05Z |
| format | Article |
| id | doaj.art-fa36f19962ac4d1bbc7b8203bd97caea |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-10T10:19:05Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Catalysts |
| spelling | doaj.art-fa36f19962ac4d1bbc7b8203bd97caea2023-11-22T00:35:52ZengMDPI AGCatalysts2073-43442021-06-0111674410.3390/catal11060744Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase ActivityLaura Chronopoulou0Viviana Couto Sayalero1Hassan Rahimi2Aurelia Rughetti3Cleofe Palocci4Department of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185 Rome, ItalyDepartment of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185 Rome, ItalyDepartment of Experimental Medicine, University of Rome La Sapienza, Viale Regina Elena 324, 00161 Rome, ItalyDepartment of Experimental Medicine, University of Rome La Sapienza, Viale Regina Elena 324, 00161 Rome, ItalyDepartment of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185 Rome, ItalyRecent advances in nanotechnology techniques enable the production of polymeric nanoparticles with specific morphologies and dimensions and, by tailoring their surfaces, one can manipulate their characteristics to suit specific applications. In this work we report an innovative approach for the immobilization of a commercial lipase from <i>Candida rugosa</i> (CRL) which employs nanostructured polymeric carriers conjugated with anti-lipase monoclonal antibodies (MoAbs). MoAbs were chemically conjugated on the surface of polymeric nanoparticles and used to selectively adsorb CRL molecules. Hydrolytic enzymatic assays evidenced that such immobilization technique afforded a significant enhancement of enzymatic activity in comparison to the free enzyme.https://www.mdpi.com/2073-4344/11/6/744lipasemonoclonal antibodiesenzyme immobilizationpolymeric nanoparticles |
| spellingShingle | Laura Chronopoulou Viviana Couto Sayalero Hassan Rahimi Aurelia Rughetti Cleofe Palocci Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity Catalysts lipase monoclonal antibodies enzyme immobilization polymeric nanoparticles |
| title | Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity |
| title_full | Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity |
| title_fullStr | Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity |
| title_full_unstemmed | Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity |
| title_short | Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity |
| title_sort | polymeric nanoparticles decorated with monoclonal antibodies a new immobilization strategy for increasing lipase activity |
| topic | lipase monoclonal antibodies enzyme immobilization polymeric nanoparticles |
| url | https://www.mdpi.com/2073-4344/11/6/744 |
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