Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens
Delineating the complex network of interactions between antigen-specific T cells and antigen presenting cells (APCs) is crucial for effective precision therapies against cancer, chronic infections, and autoimmunity. However, the existing arsenal for examining antigen-specific T cell interactions is...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2024-01-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/91809 |
| _version_ | 1797351999546589184 |
|---|---|
| author | Munir Akkaya Jafar Al Souz Daniel Williams Rahul Kamdar Olena Kamenyeva Juraj Kabat Ethan Shevach Billur Akkaya |
| author_facet | Munir Akkaya Jafar Al Souz Daniel Williams Rahul Kamdar Olena Kamenyeva Juraj Kabat Ethan Shevach Billur Akkaya |
| author_sort | Munir Akkaya |
| collection | DOAJ |
| description | Delineating the complex network of interactions between antigen-specific T cells and antigen presenting cells (APCs) is crucial for effective precision therapies against cancer, chronic infections, and autoimmunity. However, the existing arsenal for examining antigen-specific T cell interactions is restricted to a select few antigen-T cell receptor pairs, with limited in situ utility. This lack of versatility is largely due to the disruptive effects of reagents on the immune synapse, which hinder real-time monitoring of antigen-specific interactions. To address this limitation, we have developed a novel and versatile immune monitoring strategy by adding a short cysteine-rich tag to antigenic peptides that emits fluorescence upon binding to thiol-reactive biarsenical hairpin compounds. Our findings demonstrate the specificity and durability of the novel antigen-targeting probes during dynamic immune monitoring in vitro and in vivo. This strategy opens new avenues for biological validation of T-cell receptors with newly identified epitopes by revealing the behavior of previously unrecognized antigen-receptor pairs, expanding our understanding of T cell responses. |
| first_indexed | 2024-03-08T13:09:47Z |
| format | Article |
| id | doaj.art-fa37a84c2daa401bbccbb746c12a3d89 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-08T13:09:47Z |
| publishDate | 2024-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-fa37a84c2daa401bbccbb746c12a3d892024-01-18T15:46:21ZengeLife Sciences Publications LtdeLife2050-084X2024-01-011210.7554/eLife.91809Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigensMunir Akkaya0Jafar Al Souz1Daniel Williams2Rahul Kamdar3Olena Kamenyeva4https://orcid.org/0000-0002-6541-5616Juraj Kabat5Ethan Shevach6Billur Akkaya7https://orcid.org/0000-0002-6808-3776Department of Internal Medicine, Division of Rheumatology and Immunology, The College of Medicine, The Ohio State University, Columbus, United States; Microbial Infection and Immunity, The Ohio State University Wexner Medical Center, Columbus, United States; Pelotonia Institute for Immuno-Oncology, The Ohio State University, Columbus, United StatesNational Institute of Allergy and Infectious Diseases, Bethesda, United StatesNational Institute of Allergy and Infectious Diseases, Bethesda, United StatesNational Institute of Allergy and Infectious Diseases, Bethesda, United StatesNational Institute of Allergy and Infectious Diseases, Bethesda, United StatesNational Institute of Allergy and Infectious Diseases, Bethesda, United StatesNational Institute of Allergy and Infectious Diseases, Bethesda, United StatesPelotonia Institute for Immuno-Oncology, The Ohio State University, Columbus, United States; Department of Neurology, The Ohio State University Wexner Medical Center, Columbus, United StatesDelineating the complex network of interactions between antigen-specific T cells and antigen presenting cells (APCs) is crucial for effective precision therapies against cancer, chronic infections, and autoimmunity. However, the existing arsenal for examining antigen-specific T cell interactions is restricted to a select few antigen-T cell receptor pairs, with limited in situ utility. This lack of versatility is largely due to the disruptive effects of reagents on the immune synapse, which hinder real-time monitoring of antigen-specific interactions. To address this limitation, we have developed a novel and versatile immune monitoring strategy by adding a short cysteine-rich tag to antigenic peptides that emits fluorescence upon binding to thiol-reactive biarsenical hairpin compounds. Our findings demonstrate the specificity and durability of the novel antigen-targeting probes during dynamic immune monitoring in vitro and in vivo. This strategy opens new avenues for biological validation of T-cell receptors with newly identified epitopes by revealing the behavior of previously unrecognized antigen-receptor pairs, expanding our understanding of T cell responses.https://elifesciences.org/articles/91809t celldendritic cellimmune responseantigenimmune synapse |
| spellingShingle | Munir Akkaya Jafar Al Souz Daniel Williams Rahul Kamdar Olena Kamenyeva Juraj Kabat Ethan Shevach Billur Akkaya Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens eLife t cell dendritic cell immune response antigen immune synapse |
| title | Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens |
| title_full | Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens |
| title_fullStr | Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens |
| title_full_unstemmed | Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens |
| title_short | Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens |
| title_sort | illuminating t cell dendritic cell interactions in vivo by flashing antigens |
| topic | t cell dendritic cell immune response antigen immune synapse |
| url | https://elifesciences.org/articles/91809 |
| work_keys_str_mv | AT munirakkaya illuminatingtcelldendriticcellinteractionsinvivobyflashingantigens AT jafaralsouz illuminatingtcelldendriticcellinteractionsinvivobyflashingantigens AT danielwilliams illuminatingtcelldendriticcellinteractionsinvivobyflashingantigens AT rahulkamdar illuminatingtcelldendriticcellinteractionsinvivobyflashingantigens AT olenakamenyeva illuminatingtcelldendriticcellinteractionsinvivobyflashingantigens AT jurajkabat illuminatingtcelldendriticcellinteractionsinvivobyflashingantigens AT ethanshevach illuminatingtcelldendriticcellinteractionsinvivobyflashingantigens AT billurakkaya illuminatingtcelldendriticcellinteractionsinvivobyflashingantigens |