OPTIMIZING THE USE OF DUDDINGTONIA FLAGRANS CHLAMYDOSPORES AGAINST HAEMONCHUS CONTORTUS IN FECES OF SHEEP
The aim of this study was to identify the proportion of D. flagrans chlamydospores that led to the highest trapping efficacy in the presence of eggs or L3 larvae of H. contortus. Firstly, five groups of 15 coprocultures each were prepared, with proportions of eggs:chlamydospores (E:C) in Groups I th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad Autónoma de Yucatán
2015-12-01
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| Series: | Tropical and Subtropical Agroecosystems |
| Subjects: | |
| Online Access: | http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/2115 |
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| author | Nadia Florencia Ojeda-Robertos Juan Felipe de Jesus Torres-Acosta Pedro Mendoza-de-Gives Roberto Gonzalez-Garduño Rosa Ofelia Valero-Coss Enrique Liebano-Hernandez Armin Ayala-Burgos |
| author_facet | Nadia Florencia Ojeda-Robertos Juan Felipe de Jesus Torres-Acosta Pedro Mendoza-de-Gives Roberto Gonzalez-Garduño Rosa Ofelia Valero-Coss Enrique Liebano-Hernandez Armin Ayala-Burgos |
| author_sort | Nadia Florencia Ojeda-Robertos |
| collection | DOAJ |
| description | The aim of this study was to identify the proportion of D. flagrans chlamydospores that led to the highest trapping efficacy in the presence of eggs or L3 larvae of H. contortus. Firstly, five groups of 15 coprocultures each were prepared, with proportions of eggs:chlamydospores (E:C) in Groups I throught V of 1:0 (Control group), Â 1:1, 1:10, Â 1:100, and 1:1.000, respectively. A second batch of fifty coprocultures was then prepared, with five groups of 10 coprocultures each and larvae:chlamydospore (L:C) ratios equal to those used to evaluate different E:C ratios (Groups VI, VII, VIII, IX and X). Larval reduction percentages were calculated to estimate trapping efficacy. Numbers of larvae harvested from cultures seeded with eggs and chlamydospores at E:C ratios of less than 1:1 were lower than those from the control (P<0.05). Â Similarly, when L3 larvae were used as bait, the reduction percentage initially increased with increases in chlamydospore density, but efficacy did not continue to increase at L:C proportions beyond 1:10 (P>0.05). This study demonstrates the importance of adjusting the dose of D. flagrans chlamydospores to achieve 10 chlamydospores for each egg or larvae in the faeces, in order to reach the optimal use. |
| first_indexed | 2024-12-19T20:27:14Z |
| format | Article |
| id | doaj.art-fa37bfd15d414cb197685a302d4c968c |
| institution | Directory Open Access Journal |
| issn | 1870-0462 |
| language | English |
| last_indexed | 2024-12-19T20:27:14Z |
| publishDate | 2015-12-01 |
| publisher | Universidad Autónoma de Yucatán |
| record_format | Article |
| series | Tropical and Subtropical Agroecosystems |
| spelling | doaj.art-fa37bfd15d414cb197685a302d4c968c2022-12-21T20:06:48ZengUniversidad Autónoma de YucatánTropical and Subtropical Agroecosystems1870-04622015-12-01183667OPTIMIZING THE USE OF DUDDINGTONIA FLAGRANS CHLAMYDOSPORES AGAINST HAEMONCHUS CONTORTUS IN FECES OF SHEEPNadia Florencia Ojeda-Robertos0Juan Felipe de Jesus Torres-Acosta1Pedro Mendoza-de-Gives2Roberto Gonzalez-Garduño3Rosa Ofelia Valero-Coss4Enrique Liebano-Hernandez5Armin Ayala-Burgos6Universidad Juarez Autonoma de Tabasco. División Académica Ciencias AgropecuariasFMVZ, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, Yucatán. MéxicoCentro Nacional de Investigación Disciplinaria en ParasitologÃa Veterinaria, INIFAP. Jiutepec, MorelosUniversidad Autónoma Chapingo, Unidad Regional Sur Sureste. Teapa, Tabasco, MéxicoCentro Nacional de Investigación Disciplinaria en ParasitologÃa Veterinaria, INIFAP. Jiutepec, MorelosCentro Nacional de Investigación Disciplinaria en ParasitologÃa Veterinaria, INIFAP. Jiutepec, MorelosFMVZ, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, Yucatán. MéxicoThe aim of this study was to identify the proportion of D. flagrans chlamydospores that led to the highest trapping efficacy in the presence of eggs or L3 larvae of H. contortus. Firstly, five groups of 15 coprocultures each were prepared, with proportions of eggs:chlamydospores (E:C) in Groups I throught V of 1:0 (Control group),  1:1, 1:10,  1:100, and 1:1.000, respectively. A second batch of fifty coprocultures was then prepared, with five groups of 10 coprocultures each and larvae:chlamydospore (L:C) ratios equal to those used to evaluate different E:C ratios (Groups VI, VII, VIII, IX and X). Larval reduction percentages were calculated to estimate trapping efficacy. Numbers of larvae harvested from cultures seeded with eggs and chlamydospores at E:C ratios of less than 1:1 were lower than those from the control (P<0.05).  Similarly, when L3 larvae were used as bait, the reduction percentage initially increased with increases in chlamydospore density, but efficacy did not continue to increase at L:C proportions beyond 1:10 (P>0.05). This study demonstrates the importance of adjusting the dose of D. flagrans chlamydospores to achieve 10 chlamydospores for each egg or larvae in the faeces, in order to reach the optimal use.http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/2115ChlamydosporesDuddingtonia flagransHaemonchus contortusoptimal dose |
| spellingShingle | Nadia Florencia Ojeda-Robertos Juan Felipe de Jesus Torres-Acosta Pedro Mendoza-de-Gives Roberto Gonzalez-Garduño Rosa Ofelia Valero-Coss Enrique Liebano-Hernandez Armin Ayala-Burgos OPTIMIZING THE USE OF DUDDINGTONIA FLAGRANS CHLAMYDOSPORES AGAINST HAEMONCHUS CONTORTUS IN FECES OF SHEEP Tropical and Subtropical Agroecosystems Chlamydospores Duddingtonia flagrans Haemonchus contortus optimal dose |
| title | OPTIMIZING THE USE OF DUDDINGTONIA FLAGRANS CHLAMYDOSPORES AGAINST HAEMONCHUS CONTORTUS IN FECES OF SHEEP |
| title_full | OPTIMIZING THE USE OF DUDDINGTONIA FLAGRANS CHLAMYDOSPORES AGAINST HAEMONCHUS CONTORTUS IN FECES OF SHEEP |
| title_fullStr | OPTIMIZING THE USE OF DUDDINGTONIA FLAGRANS CHLAMYDOSPORES AGAINST HAEMONCHUS CONTORTUS IN FECES OF SHEEP |
| title_full_unstemmed | OPTIMIZING THE USE OF DUDDINGTONIA FLAGRANS CHLAMYDOSPORES AGAINST HAEMONCHUS CONTORTUS IN FECES OF SHEEP |
| title_short | OPTIMIZING THE USE OF DUDDINGTONIA FLAGRANS CHLAMYDOSPORES AGAINST HAEMONCHUS CONTORTUS IN FECES OF SHEEP |
| title_sort | optimizing the use of duddingtonia flagrans chlamydospores against haemonchus contortus in feces of sheep |
| topic | Chlamydospores Duddingtonia flagrans Haemonchus contortus optimal dose |
| url | http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/2115 |
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