Childhood Obesity-Related Mechanisms: MicroRNome and Transcriptome Changes in a Nested Case-Control Study
Childhood obesity could contribute to adulthood obesity, leading to adverse health outcomes in adults. However, the mechanisms for how obesity is developed are still unclear. To determine the epigenome-wide and genome-wide expression changes related with childhood obesity, we compared microRNome and...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/8/878 |
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| author | Jin Hee Kim Da Hae Kim Youn-Hee Lim Choong Ho Shin Young Ah Lee Bung-Nyun Kim Johanna Inhyang Kim Yun-Chul Hong |
| author_facet | Jin Hee Kim Da Hae Kim Youn-Hee Lim Choong Ho Shin Young Ah Lee Bung-Nyun Kim Johanna Inhyang Kim Yun-Chul Hong |
| author_sort | Jin Hee Kim |
| collection | DOAJ |
| description | Childhood obesity could contribute to adulthood obesity, leading to adverse health outcomes in adults. However, the mechanisms for how obesity is developed are still unclear. To determine the epigenome-wide and genome-wide expression changes related with childhood obesity, we compared microRNome and transcriptome levels as well as leptin protein levels in whole bloods of 12 obese and 24 normal children aged 6 years. miR-328-3p, miR-1301-3p, miR-4685-3p, and miR-6803-3p were negatively associated with all obesity indicators. The four miRNAs were also associated with 3948 mRNAs, and separate 475 mRNAs (185 among 3948 mRNAs) were associated with all obesity indicators. The 2533 mRNAs (64.2%) among the 3948 mRNAs and 286 mRNAs (60.2%) among the 475 mRNAs were confirmed as targets of the four miRNAs in public databases through miRWalk 2.0. Leptin protein was associated with miR-6803-3p negatively and all obesity indicators positively. Using DAVID bioinformatics resources 6.8, top three pathways for obesity-related gene set were metabolic pathways, pathways in cancer, and PI3K-Akt signaling pathway. The top three obesity-related disease classes were metabolic, cardiovascular, and chemdependency. Our results support that childhood obesity could be developed through miRNAs-related epigenetic mechanism and, further, these obesity-related epigenetic changes could control the pathways related with the development of various diseases. |
| first_indexed | 2024-03-10T08:59:57Z |
| format | Article |
| id | doaj.art-fa387c672cb24498abfb2584eafa7933 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T08:59:57Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-fa387c672cb24498abfb2584eafa79332023-11-22T06:51:24ZengMDPI AGBiomedicines2227-90592021-07-019887810.3390/biomedicines9080878Childhood Obesity-Related Mechanisms: MicroRNome and Transcriptome Changes in a Nested Case-Control StudyJin Hee Kim0Da Hae Kim1Youn-Hee Lim2Choong Ho Shin3Young Ah Lee4Bung-Nyun Kim5Johanna Inhyang Kim6Yun-Chul Hong7Department of Integrative Bioscience & Biotechnology, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, KoreaDepartment of Integrative Bioscience & Biotechnology, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, KoreaInstitute of Environmental Medicine, Seoul National University Medical Research Center, Seoul 03080, KoreaDepartment of Pediatrics, Seoul National University College of Medicine, Seoul 03080, KoreaDepartment of Pediatrics, Seoul National University College of Medicine, Seoul 03080, KoreaDivision of Children and Adolescent Psychiatry, Department of Psychiatry, Seoul National University Hospital, Seoul 03080, KoreaDepartment of Psychiatry, Hanyang University Medical Center, Seoul 04763, KoreaInstitute of Environmental Medicine, Seoul National University Medical Research Center, Seoul 03080, KoreaChildhood obesity could contribute to adulthood obesity, leading to adverse health outcomes in adults. However, the mechanisms for how obesity is developed are still unclear. To determine the epigenome-wide and genome-wide expression changes related with childhood obesity, we compared microRNome and transcriptome levels as well as leptin protein levels in whole bloods of 12 obese and 24 normal children aged 6 years. miR-328-3p, miR-1301-3p, miR-4685-3p, and miR-6803-3p were negatively associated with all obesity indicators. The four miRNAs were also associated with 3948 mRNAs, and separate 475 mRNAs (185 among 3948 mRNAs) were associated with all obesity indicators. The 2533 mRNAs (64.2%) among the 3948 mRNAs and 286 mRNAs (60.2%) among the 475 mRNAs were confirmed as targets of the four miRNAs in public databases through miRWalk 2.0. Leptin protein was associated with miR-6803-3p negatively and all obesity indicators positively. Using DAVID bioinformatics resources 6.8, top three pathways for obesity-related gene set were metabolic pathways, pathways in cancer, and PI3K-Akt signaling pathway. The top three obesity-related disease classes were metabolic, cardiovascular, and chemdependency. Our results support that childhood obesity could be developed through miRNAs-related epigenetic mechanism and, further, these obesity-related epigenetic changes could control the pathways related with the development of various diseases.https://www.mdpi.com/2227-9059/9/8/878childrenobesitymiRNAmRNAomicspathway |
| spellingShingle | Jin Hee Kim Da Hae Kim Youn-Hee Lim Choong Ho Shin Young Ah Lee Bung-Nyun Kim Johanna Inhyang Kim Yun-Chul Hong Childhood Obesity-Related Mechanisms: MicroRNome and Transcriptome Changes in a Nested Case-Control Study Biomedicines children obesity miRNA mRNA omics pathway |
| title | Childhood Obesity-Related Mechanisms: MicroRNome and Transcriptome Changes in a Nested Case-Control Study |
| title_full | Childhood Obesity-Related Mechanisms: MicroRNome and Transcriptome Changes in a Nested Case-Control Study |
| title_fullStr | Childhood Obesity-Related Mechanisms: MicroRNome and Transcriptome Changes in a Nested Case-Control Study |
| title_full_unstemmed | Childhood Obesity-Related Mechanisms: MicroRNome and Transcriptome Changes in a Nested Case-Control Study |
| title_short | Childhood Obesity-Related Mechanisms: MicroRNome and Transcriptome Changes in a Nested Case-Control Study |
| title_sort | childhood obesity related mechanisms micrornome and transcriptome changes in a nested case control study |
| topic | children obesity miRNA mRNA omics pathway |
| url | https://www.mdpi.com/2227-9059/9/8/878 |
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