Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil

Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil

We typed 600 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 51 hospitals in the Rio de Janeiro, Brazil, metropolitan area during 2014–2017. We found that multiple new clonal complex (CC) 5 sequence types had replaced previously dominant MRSA lineages in hospitals. Whole-gen...

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Bibliographic Details
Main Authors: Alice Slotfeldt Viana, Ana Maria Nunes Botelho, Ahmed M. Moustafa, Craig L.K. Boge, Adriana Lucia Pires Ferreira, Maria Cícera da Silva Carvalho, Márcia Aparecida Guimarães, Bruno de Souza Scramignon Costa, Marcos Corrêa de Mattos, Sabrina Pires Maciel, Juliana Echevarria-Lima, Apurva Narechania, Kelsey O’Brien, Chanelle Ryan, Jeffrey S. Gerber, Bernadete Teixeira Ferreira Carvalho, Agnes Marie Sá Figueiredo, Paul J. Planet
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2021-11-01
Series:Emerging Infectious Diseases
Subjects:
MRSA
molecular epidemiology
phylogenetics
bloodstream infections
monocytes
phagocytosis
Online Access:https://wwwnc.cdc.gov/eid/article/27/11/21-0097_article
Description
Summary:We typed 600 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 51 hospitals in the Rio de Janeiro, Brazil, metropolitan area during 2014–2017. We found that multiple new clonal complex (CC) 5 sequence types had replaced previously dominant MRSA lineages in hospitals. Whole-genome analysis of 208 isolates revealed an emerging sublineage of multidrug-resistant MRSA, sequence type 105, staphylococcal cassette chromosome mec II, spa t002, which we designated the Rio de Janeiro (RdJ) clone. Using molecular clock analysis, we hypothesized that this lineage began to expand in the Rio de Janeiro metropolitan area in 2009. Multivariate analysis supported an association between bloodstream infections and the CC5 lineage that includes the RdJ clone. Compared with other closely related isolates, representative isolates of the RdJ clone more effectively evaded immune function related to monocytic cells, as evidenced by decreased phagocytosis rate and increased numbers of viable unphagocytosed (free) bacteria after in vitro exposure to monocytes.
ISSN:1080-6040
1080-6059

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