Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil
We typed 600 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 51 hospitals in the Rio de Janeiro, Brazil, metropolitan area during 2014–2017. We found that multiple new clonal complex (CC) 5 sequence types had replaced previously dominant MRSA lineages in hospitals. Whole-gen...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2021-11-01
|
| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/27/11/21-0097_article |
| _version_ | 1818367131251638272 |
|---|---|
| author | Alice Slotfeldt Viana Ana Maria Nunes Botelho Ahmed M. Moustafa Craig L.K. Boge Adriana Lucia Pires Ferreira Maria Cícera da Silva Carvalho Márcia Aparecida Guimarães Bruno de Souza Scramignon Costa Marcos Corrêa de Mattos Sabrina Pires Maciel Juliana Echevarria-Lima Apurva Narechania Kelsey O’Brien Chanelle Ryan Jeffrey S. Gerber Bernadete Teixeira Ferreira Carvalho Agnes Marie Sá Figueiredo Paul J. Planet |
| author_facet | Alice Slotfeldt Viana Ana Maria Nunes Botelho Ahmed M. Moustafa Craig L.K. Boge Adriana Lucia Pires Ferreira Maria Cícera da Silva Carvalho Márcia Aparecida Guimarães Bruno de Souza Scramignon Costa Marcos Corrêa de Mattos Sabrina Pires Maciel Juliana Echevarria-Lima Apurva Narechania Kelsey O’Brien Chanelle Ryan Jeffrey S. Gerber Bernadete Teixeira Ferreira Carvalho Agnes Marie Sá Figueiredo Paul J. Planet |
| author_sort | Alice Slotfeldt Viana |
| collection | DOAJ |
| description | We typed 600 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 51 hospitals in the Rio de Janeiro, Brazil, metropolitan area during 2014–2017. We found that multiple new clonal complex (CC) 5 sequence types had replaced previously dominant MRSA lineages in hospitals. Whole-genome analysis of 208 isolates revealed an emerging sublineage of multidrug-resistant MRSA, sequence type 105, staphylococcal cassette chromosome mec II, spa t002, which we designated the Rio de Janeiro (RdJ) clone. Using molecular clock analysis, we hypothesized that this lineage began to expand in the Rio de Janeiro metropolitan area in 2009. Multivariate analysis supported an association between bloodstream infections and the CC5 lineage that includes the RdJ clone. Compared with other closely related isolates, representative isolates of the RdJ clone more effectively evaded immune function related to monocytic cells, as evidenced by decreased phagocytosis rate and increased numbers of viable unphagocytosed (free) bacteria after in vitro exposure to monocytes. |
| first_indexed | 2024-12-13T22:47:10Z |
| format | Article |
| id | doaj.art-fa3a3b0598204e24a1f36b5a3cd206cb |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-13T22:47:10Z |
| publishDate | 2021-11-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-fa3a3b0598204e24a1f36b5a3cd206cb2022-12-21T23:28:42ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592021-11-0127112825283510.3201/eid2711.210097Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, BrazilAlice Slotfeldt VianaAna Maria Nunes BotelhoAhmed M. MoustafaCraig L.K. BogeAdriana Lucia Pires FerreiraMaria Cícera da Silva CarvalhoMárcia Aparecida GuimarãesBruno de Souza Scramignon CostaMarcos Corrêa de MattosSabrina Pires MacielJuliana Echevarria-LimaApurva NarechaniaKelsey O’BrienChanelle RyanJeffrey S. GerberBernadete Teixeira Ferreira CarvalhoAgnes Marie Sá FigueiredoPaul J. PlanetWe typed 600 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 51 hospitals in the Rio de Janeiro, Brazil, metropolitan area during 2014–2017. We found that multiple new clonal complex (CC) 5 sequence types had replaced previously dominant MRSA lineages in hospitals. Whole-genome analysis of 208 isolates revealed an emerging sublineage of multidrug-resistant MRSA, sequence type 105, staphylococcal cassette chromosome mec II, spa t002, which we designated the Rio de Janeiro (RdJ) clone. Using molecular clock analysis, we hypothesized that this lineage began to expand in the Rio de Janeiro metropolitan area in 2009. Multivariate analysis supported an association between bloodstream infections and the CC5 lineage that includes the RdJ clone. Compared with other closely related isolates, representative isolates of the RdJ clone more effectively evaded immune function related to monocytic cells, as evidenced by decreased phagocytosis rate and increased numbers of viable unphagocytosed (free) bacteria after in vitro exposure to monocytes.https://wwwnc.cdc.gov/eid/article/27/11/21-0097_articleMRSAmolecular epidemiologyphylogeneticsbloodstream infectionsmonocytesphagocytosis |
| spellingShingle | Alice Slotfeldt Viana Ana Maria Nunes Botelho Ahmed M. Moustafa Craig L.K. Boge Adriana Lucia Pires Ferreira Maria Cícera da Silva Carvalho Márcia Aparecida Guimarães Bruno de Souza Scramignon Costa Marcos Corrêa de Mattos Sabrina Pires Maciel Juliana Echevarria-Lima Apurva Narechania Kelsey O’Brien Chanelle Ryan Jeffrey S. Gerber Bernadete Teixeira Ferreira Carvalho Agnes Marie Sá Figueiredo Paul J. Planet Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil Emerging Infectious Diseases MRSA molecular epidemiology phylogenetics bloodstream infections monocytes phagocytosis |
| title | Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil |
| title_full | Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil |
| title_fullStr | Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil |
| title_full_unstemmed | Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil |
| title_short | Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil |
| title_sort | multidrug resistant methicillin resistant staphylococcus aureus associated with bacteremia and monocyte evasion rio de janeiro brazil |
| topic | MRSA molecular epidemiology phylogenetics bloodstream infections monocytes phagocytosis |
| url | https://wwwnc.cdc.gov/eid/article/27/11/21-0097_article |
| work_keys_str_mv | AT aliceslotfeldtviana multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT anamarianunesbotelho multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT ahmedmmoustafa multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT craiglkboge multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT adrianaluciapiresferreira multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT mariaciceradasilvacarvalho multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT marciaaparecidaguimaraes multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT brunodesouzascramignoncosta multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT marcoscorreademattos multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT sabrinapiresmaciel multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT julianaechevarrialima multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT apurvanarechania multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT kelseyobrien multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT chanelleryan multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT jeffreysgerber multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT bernadeteteixeiraferreiracarvalho multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT agnesmariesafigueiredo multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil AT pauljplanet multidrugresistantmethicillinresistantstaphylococcusaureusassociatedwithbacteremiaandmonocyteevasionriodejaneirobrazil |