Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil
ABSTRACT Accurate insect specimen identification is usually a crucial first step in a forensic entomological analysis. It is traditionally done by morphological classification using identification keys. However, due to sensibility limitations in the identification of animal species based only on the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2017-05-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100403&lng=en&tlng=en |
| _version_ | 1811200792216993792 |
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| author | Pablo Viana Oliveira Natan Silva Matos Angélica Hollunder Klippel Janyra Oliveira-Costa Francisco de Paula Careta Greiciane Gaburro Paneto |
| author_facet | Pablo Viana Oliveira Natan Silva Matos Angélica Hollunder Klippel Janyra Oliveira-Costa Francisco de Paula Careta Greiciane Gaburro Paneto |
| author_sort | Pablo Viana Oliveira |
| collection | DOAJ |
| description | ABSTRACT Accurate insect specimen identification is usually a crucial first step in a forensic entomological analysis. It is traditionally done by morphological classification using identification keys. However, due to sensibility limitations in the identification of animal species based only on their morphology, new methods have been developed, including species identification by DNA barcodes. The objective of this study was to identify forensically important species of Diptera in Espirito Santo state using DNA barcodes. For this, adult flies were collected in Espirito Santo, Southeast Region of Brazil. After DNA extraction, COI gene was amplified and sequenced. All sequences were matched to BOLD platform and alternatively to GenBank MegaBLAST. As result, 281 adult flies were collected and identified morphologically. From these, 36% of samples were classified as Calliphoridae, 34% of Muscidae and 30% of Sarcophagidae. Approximately 10% of all collected samples were analyzes by DNA. It was possible to identify only 35.7% of tested samples, probably due to lack of samples deposited in databases. Therefore, more efforts should be made to deposit a greater variety of dipterous in databases to allow the use of this technique in forensic routine, especially in BOLD. |
| first_indexed | 2024-04-12T02:09:39Z |
| format | Article |
| id | doaj.art-fa3df580136b4f68892bee345ac7d378 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-12T02:09:39Z |
| publishDate | 2017-05-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-fa3df580136b4f68892bee345ac7d3782022-12-22T03:52:27ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242017-05-0160010.1590/1678-4324-2017160106S1516-89132017000100403Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, BrazilPablo Viana OliveiraNatan Silva MatosAngélica Hollunder KlippelJanyra Oliveira-CostaFrancisco de Paula CaretaGreiciane Gaburro PanetoABSTRACT Accurate insect specimen identification is usually a crucial first step in a forensic entomological analysis. It is traditionally done by morphological classification using identification keys. However, due to sensibility limitations in the identification of animal species based only on their morphology, new methods have been developed, including species identification by DNA barcodes. The objective of this study was to identify forensically important species of Diptera in Espirito Santo state using DNA barcodes. For this, adult flies were collected in Espirito Santo, Southeast Region of Brazil. After DNA extraction, COI gene was amplified and sequenced. All sequences were matched to BOLD platform and alternatively to GenBank MegaBLAST. As result, 281 adult flies were collected and identified morphologically. From these, 36% of samples were classified as Calliphoridae, 34% of Muscidae and 30% of Sarcophagidae. Approximately 10% of all collected samples were analyzes by DNA. It was possible to identify only 35.7% of tested samples, probably due to lack of samples deposited in databases. Therefore, more efforts should be made to deposit a greater variety of dipterous in databases to allow the use of this technique in forensic routine, especially in BOLD.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100403&lng=en&tlng=enEntomologyCOIBOLDForensicFlies |
| spellingShingle | Pablo Viana Oliveira Natan Silva Matos Angélica Hollunder Klippel Janyra Oliveira-Costa Francisco de Paula Careta Greiciane Gaburro Paneto Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil Brazilian Archives of Biology and Technology Entomology COI BOLD Forensic Flies |
| title | Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil |
| title_full | Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil |
| title_fullStr | Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil |
| title_full_unstemmed | Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil |
| title_short | Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil |
| title_sort | using dna barcodes to identify forensically important species of diptera in espirito santo state brazil |
| topic | Entomology COI BOLD Forensic Flies |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100403&lng=en&tlng=en |
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