Genetic diversity of different breeds of Kazakh sheep using microsatellite analysis
<p>A total of 75 individuals from five sheep populations in Kazakhstan were investigated based on 12 STR (short tandem repeat, also known as microsatellite) markers in order to study their genetic structure and phylogenetic relationship based on genetic distances. These sheep had a high level...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2019-06-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://www.arch-anim-breed.net/62/305/2019/aab-62-305-2019.pdf |
| Summary: | <p>A total of 75 individuals from five sheep populations in Kazakhstan
were investigated based on 12 STR (short tandem repeat, also known as microsatellite) markers in order to study their genetic
structure and phylogenetic relationship based on genetic distances. These
sheep had a high level of genetic diversity. In total, 163 alleles were
found in all the populations using 12 microsatellite loci. The mean
number of alleles, effective number of alleles, and polymorphism information content (PIC) values per loci were
13.4, 5.9, and 0.78, respectively. Comparing the allelic diversity between
the populations, the highest genetic diversity was observed in the Edilbay-1 sheep breed (<span class="inline-formula">8.333±0.644</span>), and the lowest parameter was for Kazakh
Arkhar-Merino (<span class="inline-formula">7.083±0.633</span>). In all populations, there is a
deficiency of heterozygosity. The largest genetic diversity was found in
loci <i>INRA023</i> and <i>CSRD247</i> with 16 alleles, and the smallest polymorphism was noted for the
locus <i>D5S2</i> with 8 alleles. The level of observed heterozygosity was in the range <span class="inline-formula">0.678±0.051</span> for Kazakh Arkhar-Merino and <span class="inline-formula">0.767±0.047</span> for
Kazakh fat-tailed coarse wool. The expected heterozygosity level range was from
<span class="inline-formula">0.702±0.033</span> for Kazakh Arkhar-Merino to <span class="inline-formula">0.777±0.023</span> for
Edilbay-1. When 12 microsatellite loci are compared, the <i>OarFCB20</i> locus showed the
highest level of genetic variability. Excess of heterozygosity was observed
at three loci; <i>MAF065</i>, <i>McM042</i>, and <i>OarFCB20</i>.
The highest genetic distance was observed between
Kazakh Arkhar-Merino and Edilbay-1, whereas the genetic distance between
Edilbay-1 and Edilbay-2 is the smallest using Nei's standard genetic
distance. The Edilbay-1 sheep breed possesses the largest genetic diversity
among these five populations.</p> |
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| ISSN: | 0003-9438 2363-9822 |