Bacteriophage ZCSE2 is a Potent Antimicrobial against <i>Salmonella enterica</i> Serovars: Ultrastructure, Genomics and Efficacy
Developing novel antimicrobials capable of controlling multidrug-resistant bacterial pathogens is essential to restrict the use of antibiotics. Bacteriophages (phages) constitute a major resource that can be harnessed as an alternative to traditional antimicrobial therapies. Phage ZCSE2 was isolated...
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MDPI AG
2020-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/4/424 |
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| author | Ahmed Mohamed Omar Taha Hesham M. El-Sherif Phillippa L. Connerton Steven P.T. Hooton Nabil D. Bassim Ian F. Connerton Ayman El-Shibiny |
| author_facet | Ahmed Mohamed Omar Taha Hesham M. El-Sherif Phillippa L. Connerton Steven P.T. Hooton Nabil D. Bassim Ian F. Connerton Ayman El-Shibiny |
| author_sort | Ahmed Mohamed |
| collection | DOAJ |
| description | Developing novel antimicrobials capable of controlling multidrug-resistant bacterial pathogens is essential to restrict the use of antibiotics. Bacteriophages (phages) constitute a major resource that can be harnessed as an alternative to traditional antimicrobial therapies. Phage ZCSE2 was isolated among several others from raw sewage but was distinguished by broad-spectrum activity against <i>Salmonella</i> serovars considered pathogenic to humans and animals. Lytic profiles of ZCSE2 against a panel of <i>Salmonella</i> were determined together with low temperature activity and pH stability. The morphological features of the phage and host infection processes were characterized using a combination of transmission electron and atomic force microscopies. Whole genome sequencing of ZCSE2 produced a complete DNA sequence of 53,965 bp. No known virulence genes were identified in the sequence data, making ZCSE2 a good candidate for phage-mediated biological control purposes. ZCSE2 was further tested against <i>S.</i> Enteritidis in liquid culture and was observed to reduce the target bacterium to below the limits of detection from initial concentrations of 10<sup>7</sup>–10<sup>8</sup> Colony Forming Units (CFU)/mL. With a broad host-range against pathogenic <i>Salmonella</i> serovars, phage ZCSE2 constitutes a potential tool against a major cause of human and animal disease. |
| first_indexed | 2024-03-10T20:34:27Z |
| format | Article |
| id | doaj.art-fa3e81516bed4210bba624d32e15e182 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:34:27Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-fa3e81516bed4210bba624d32e15e1822023-11-19T21:08:49ZengMDPI AGViruses1999-49152020-04-0112442410.3390/v12040424Bacteriophage ZCSE2 is a Potent Antimicrobial against <i>Salmonella enterica</i> Serovars: Ultrastructure, Genomics and EfficacyAhmed Mohamed0Omar Taha1Hesham M. El-Sherif2Phillippa L. Connerton3Steven P.T. Hooton4Nabil D. Bassim5Ian F. Connerton6Ayman El-Shibiny7Center for Microbiology and Phage Therapy, Biomedical Sciences, Zewail City of Science and Technology, Giza 12578, EgyptCenter for Microbiology and Phage Therapy, Biomedical Sciences, Zewail City of Science and Technology, Giza 12578, EgyptMechanical Design and Production Engineering Department, Faculty of Engineering, Cairo University, Giza 12613, EgyptDivision of Microbiology, Brewing and Biotechnology, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UKDivision of Microbiology, Brewing and Biotechnology, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UKMaterials Science and Engineering Department, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L8, CanadaDivision of Microbiology, Brewing and Biotechnology, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UKCenter for Microbiology and Phage Therapy, Biomedical Sciences, Zewail City of Science and Technology, Giza 12578, EgyptDeveloping novel antimicrobials capable of controlling multidrug-resistant bacterial pathogens is essential to restrict the use of antibiotics. Bacteriophages (phages) constitute a major resource that can be harnessed as an alternative to traditional antimicrobial therapies. Phage ZCSE2 was isolated among several others from raw sewage but was distinguished by broad-spectrum activity against <i>Salmonella</i> serovars considered pathogenic to humans and animals. Lytic profiles of ZCSE2 against a panel of <i>Salmonella</i> were determined together with low temperature activity and pH stability. The morphological features of the phage and host infection processes were characterized using a combination of transmission electron and atomic force microscopies. Whole genome sequencing of ZCSE2 produced a complete DNA sequence of 53,965 bp. No known virulence genes were identified in the sequence data, making ZCSE2 a good candidate for phage-mediated biological control purposes. ZCSE2 was further tested against <i>S.</i> Enteritidis in liquid culture and was observed to reduce the target bacterium to below the limits of detection from initial concentrations of 10<sup>7</sup>–10<sup>8</sup> Colony Forming Units (CFU)/mL. With a broad host-range against pathogenic <i>Salmonella</i> serovars, phage ZCSE2 constitutes a potential tool against a major cause of human and animal disease.https://www.mdpi.com/1999-4915/12/4/424bacteriophage<i>Salmonella</i>phage biocontrolatomic force microscopy |
| spellingShingle | Ahmed Mohamed Omar Taha Hesham M. El-Sherif Phillippa L. Connerton Steven P.T. Hooton Nabil D. Bassim Ian F. Connerton Ayman El-Shibiny Bacteriophage ZCSE2 is a Potent Antimicrobial against <i>Salmonella enterica</i> Serovars: Ultrastructure, Genomics and Efficacy Viruses bacteriophage <i>Salmonella</i> phage biocontrol atomic force microscopy |
| title | Bacteriophage ZCSE2 is a Potent Antimicrobial against <i>Salmonella enterica</i> Serovars: Ultrastructure, Genomics and Efficacy |
| title_full | Bacteriophage ZCSE2 is a Potent Antimicrobial against <i>Salmonella enterica</i> Serovars: Ultrastructure, Genomics and Efficacy |
| title_fullStr | Bacteriophage ZCSE2 is a Potent Antimicrobial against <i>Salmonella enterica</i> Serovars: Ultrastructure, Genomics and Efficacy |
| title_full_unstemmed | Bacteriophage ZCSE2 is a Potent Antimicrobial against <i>Salmonella enterica</i> Serovars: Ultrastructure, Genomics and Efficacy |
| title_short | Bacteriophage ZCSE2 is a Potent Antimicrobial against <i>Salmonella enterica</i> Serovars: Ultrastructure, Genomics and Efficacy |
| title_sort | bacteriophage zcse2 is a potent antimicrobial against i salmonella enterica i serovars ultrastructure genomics and efficacy |
| topic | bacteriophage <i>Salmonella</i> phage biocontrol atomic force microscopy |
| url | https://www.mdpi.com/1999-4915/12/4/424 |
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