Methicillin-resistant Staphylococcus aureus nasal carriage in international medical conference attendees
Background: Carriage of methicillin-resistant Staphylococcus aureus (MRSA) is associated with its transmission. International travels and massive gatherings may accelerate such transmission. MRSA carriage was surveyed among the attendees of two international medical conferences held in Taipei in 201...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | Journal of Microbiology, Immunology and Infection |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118218303141 |
| _version_ | 1818695296533659648 |
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| author | Yhu-Chering Huang Lin-Hui Su Tsu-Lan Wu Tzou-Yien Lin |
| author_facet | Yhu-Chering Huang Lin-Hui Su Tsu-Lan Wu Tzou-Yien Lin |
| author_sort | Yhu-Chering Huang |
| collection | DOAJ |
| description | Background: Carriage of methicillin-resistant Staphylococcus aureus (MRSA) is associated with its transmission. International travels and massive gatherings may accelerate such transmission. MRSA carriage was surveyed among the attendees of two international medical conferences held in Taipei in 2010. Methods: A total of 209 attendees from 23 countries were recruited. Nasal specimens were collected from each volunteer and subjected to polymerase chain reaction (PCR) detection for MRSA. Molecular analysis, including pulsed-field gel electrophoresis, multilocus sequence typing (MLST), typing of staphylococcal cassette chromosome mec (SCCmec) and staphylococcal protein A (spa) genes, and detection of Panton-Valentine leukocidin (PVL) and sasX genes, was performed. Results: MRSA carriage was detected in 10 (4.8%) attendees from Vietnam (3/8, 37.5%), Korea (2/6, 33.3%), Japan (2/41, 4.9%), Philippines (2/52, 3.8%), and Bangladesh (1/4, 25.0%). The proportion of MRSA colonizers was significantly higher in the local hospital group compared to those from the other groups (3/17 vs. 7/192, p < 0.05). Six MRSA isolates were available for molecular analysis. They all carried a type IV SCCmec gene. Five pulsotypes were identified; four genotypes, respectively, were identified by MLST and spa typing. None of the isolates carried either PVL or sasX genes. None of common molecular characteristics was shared by isolates from different countries. Most of these isolates were local endemic community clone in each country. Conclusions: As healthcare workers, a certain proportion of international medical conference attendees harbored MRSA in their nares, mostly local endemic community clones in each country, which has the potential of spread among attendees. Keywords: Colonization, Conference attendee, International travel, Massive gathering, Methicillin-resistant Staphylococcus aureus |
| first_indexed | 2024-12-17T13:43:13Z |
| format | Article |
| id | doaj.art-fa3f0ea2e9fc4db6a634e0325df8518c |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-17T13:43:13Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-fa3f0ea2e9fc4db6a634e0325df8518c2022-12-21T21:46:13ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822019-04-01522242247Methicillin-resistant Staphylococcus aureus nasal carriage in international medical conference attendeesYhu-Chering Huang0Lin-Hui Su1Tsu-Lan Wu2Tzou-Yien Lin3Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan; Chang Gung University College of Medicine, Kweishan, Taoyuan, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweishan, Taoyuan, Taiwan; Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Corresponding author. Department of Laboratory Medicine, Chang Gung Memorial Hospital, No. 5, Fu-Hsin Street, Kweishan, Taoyuan, 33305, Taiwan. Fax: +886 3 3971827.Department of Laboratory Medicine, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweishan, Taoyuan, TaiwanDivision of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan; Chang Gung University College of Medicine, Kweishan, Taoyuan, TaiwanBackground: Carriage of methicillin-resistant Staphylococcus aureus (MRSA) is associated with its transmission. International travels and massive gatherings may accelerate such transmission. MRSA carriage was surveyed among the attendees of two international medical conferences held in Taipei in 2010. Methods: A total of 209 attendees from 23 countries were recruited. Nasal specimens were collected from each volunteer and subjected to polymerase chain reaction (PCR) detection for MRSA. Molecular analysis, including pulsed-field gel electrophoresis, multilocus sequence typing (MLST), typing of staphylococcal cassette chromosome mec (SCCmec) and staphylococcal protein A (spa) genes, and detection of Panton-Valentine leukocidin (PVL) and sasX genes, was performed. Results: MRSA carriage was detected in 10 (4.8%) attendees from Vietnam (3/8, 37.5%), Korea (2/6, 33.3%), Japan (2/41, 4.9%), Philippines (2/52, 3.8%), and Bangladesh (1/4, 25.0%). The proportion of MRSA colonizers was significantly higher in the local hospital group compared to those from the other groups (3/17 vs. 7/192, p < 0.05). Six MRSA isolates were available for molecular analysis. They all carried a type IV SCCmec gene. Five pulsotypes were identified; four genotypes, respectively, were identified by MLST and spa typing. None of the isolates carried either PVL or sasX genes. None of common molecular characteristics was shared by isolates from different countries. Most of these isolates were local endemic community clone in each country. Conclusions: As healthcare workers, a certain proportion of international medical conference attendees harbored MRSA in their nares, mostly local endemic community clones in each country, which has the potential of spread among attendees. Keywords: Colonization, Conference attendee, International travel, Massive gathering, Methicillin-resistant Staphylococcus aureushttp://www.sciencedirect.com/science/article/pii/S1684118218303141 |
| spellingShingle | Yhu-Chering Huang Lin-Hui Su Tsu-Lan Wu Tzou-Yien Lin Methicillin-resistant Staphylococcus aureus nasal carriage in international medical conference attendees Journal of Microbiology, Immunology and Infection |
| title | Methicillin-resistant Staphylococcus aureus nasal carriage in international medical conference attendees |
| title_full | Methicillin-resistant Staphylococcus aureus nasal carriage in international medical conference attendees |
| title_fullStr | Methicillin-resistant Staphylococcus aureus nasal carriage in international medical conference attendees |
| title_full_unstemmed | Methicillin-resistant Staphylococcus aureus nasal carriage in international medical conference attendees |
| title_short | Methicillin-resistant Staphylococcus aureus nasal carriage in international medical conference attendees |
| title_sort | methicillin resistant staphylococcus aureus nasal carriage in international medical conference attendees |
| url | http://www.sciencedirect.com/science/article/pii/S1684118218303141 |
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