GEM-Gate: A Low-Cost, Flexible Approach to BioBrick Assembly
Rapid and modular assembly of DNA parts is crucial to many synthetic biologists. This can be achieved through Golden Gate assembly, which often requires purchase and delivery of new primers for each part and assembly configuration. Here, we report on a small set of primers that can be used to amplif...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | DNA |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8856/3/1/3 |
| _version_ | 1797612374900867072 |
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| author | Chloe Bower Christina Harbin Devin Camenares |
| author_facet | Chloe Bower Christina Harbin Devin Camenares |
| author_sort | Chloe Bower |
| collection | DOAJ |
| description | Rapid and modular assembly of DNA parts is crucial to many synthetic biologists. This can be achieved through Golden Gate assembly, which often requires purchase and delivery of new primers for each part and assembly configuration. Here, we report on a small set of primers that can be used to amplify any DNA from the Registry of Standard Biological Parts for Golden Gate assembly. These primers bind to regions common to the backbone plasmid for these parts, but pair imperfectly and introduce type IIS restriction enzyme sites in a way that minimizes assembly scars. This approach makes redesign of assembly strategies faster and less expensive and can help expand access to synthetic biology to a wider group of scientists and students. |
| first_indexed | 2024-03-11T06:40:24Z |
| format | Article |
| id | doaj.art-fa408fb6fe7342bea8eab844ae7c9f38 |
| institution | Directory Open Access Journal |
| issn | 2673-8856 |
| language | English |
| last_indexed | 2024-03-11T06:40:24Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | DNA |
| spelling | doaj.art-fa408fb6fe7342bea8eab844ae7c9f382023-11-17T10:39:05ZengMDPI AGDNA2673-88562023-02-0131334010.3390/dna3010003GEM-Gate: A Low-Cost, Flexible Approach to BioBrick AssemblyChloe Bower0Christina Harbin1Devin Camenares2Alma College, 614 W. Superior St, Alma, MI 48801, USAAlma College, 614 W. Superior St, Alma, MI 48801, USAAlma College, 614 W. Superior St, Alma, MI 48801, USARapid and modular assembly of DNA parts is crucial to many synthetic biologists. This can be achieved through Golden Gate assembly, which often requires purchase and delivery of new primers for each part and assembly configuration. Here, we report on a small set of primers that can be used to amplify any DNA from the Registry of Standard Biological Parts for Golden Gate assembly. These primers bind to regions common to the backbone plasmid for these parts, but pair imperfectly and introduce type IIS restriction enzyme sites in a way that minimizes assembly scars. This approach makes redesign of assembly strategies faster and less expensive and can help expand access to synthetic biology to a wider group of scientists and students.https://www.mdpi.com/2673-8856/3/1/3Golden GateassemblyPCRiGEMuniversalprimers |
| spellingShingle | Chloe Bower Christina Harbin Devin Camenares GEM-Gate: A Low-Cost, Flexible Approach to BioBrick Assembly DNA Golden Gate assembly PCR iGEM universal primers |
| title | GEM-Gate: A Low-Cost, Flexible Approach to BioBrick Assembly |
| title_full | GEM-Gate: A Low-Cost, Flexible Approach to BioBrick Assembly |
| title_fullStr | GEM-Gate: A Low-Cost, Flexible Approach to BioBrick Assembly |
| title_full_unstemmed | GEM-Gate: A Low-Cost, Flexible Approach to BioBrick Assembly |
| title_short | GEM-Gate: A Low-Cost, Flexible Approach to BioBrick Assembly |
| title_sort | gem gate a low cost flexible approach to biobrick assembly |
| topic | Golden Gate assembly PCR iGEM universal primers |
| url | https://www.mdpi.com/2673-8856/3/1/3 |
| work_keys_str_mv | AT chloebower gemgatealowcostflexibleapproachtobiobrickassembly AT christinaharbin gemgatealowcostflexibleapproachtobiobrickassembly AT devincamenares gemgatealowcostflexibleapproachtobiobrickassembly |