ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis
Cerasus humilis is a kind of economic fruit tree peculiar to China, which is widely used in the food, landscape, and pharmaceutical industries. Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration. However, the regulatory network of C. humilis in anthocyanin biosyn...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-01-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014123001450 |
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| author | Xiaolong Ji Zhe Li Mingyu Zhang Shaoyu Lang Xingshun Song |
| author_facet | Xiaolong Ji Zhe Li Mingyu Zhang Shaoyu Lang Xingshun Song |
| author_sort | Xiaolong Ji |
| collection | DOAJ |
| description | Cerasus humilis is a kind of economic fruit tree peculiar to China, which is widely used in the food, landscape, and pharmaceutical industries. Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration. However, the regulatory network of C. humilis in anthocyanin biosynthesis is still unclear. In this study, the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species. Yeast one-hybrid, dual-luciferase assays, and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C. humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS (MYB-binding elements). ChMYB1 interacted with ChbHLH42 and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT. In addition, abscisic acid (ABA) treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C. humilis fruit. Interestingly, ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42. Furthermore, ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42. Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C. humilis fruit, deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops. |
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| id | doaj.art-fa409d7d1ae84e6087e2d1e116cbe76f |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2025-03-22T04:40:03Z |
| publishDate | 2024-01-01 |
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| series | Horticultural Plant Journal |
| spelling | doaj.art-fa409d7d1ae84e6087e2d1e116cbe76f2024-04-27T23:39:13ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412024-01-011015165ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilisXiaolong Ji0Zhe Li1Mingyu Zhang2Shaoyu Lang3Xingshun Song4State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, Heilongjiang 150040, China; Department of Genetics, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, Heilongjiang 150040, China; Department of Genetics, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, Heilongjiang 150040, China; Department of Genetics, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, Heilongjiang 150040, China; Department of Genetics, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, Heilongjiang 150040, China; Department of Genetics, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, China; Corresponding author. Tel.: +86 45182191763.Cerasus humilis is a kind of economic fruit tree peculiar to China, which is widely used in the food, landscape, and pharmaceutical industries. Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration. However, the regulatory network of C. humilis in anthocyanin biosynthesis is still unclear. In this study, the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species. Yeast one-hybrid, dual-luciferase assays, and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C. humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS (MYB-binding elements). ChMYB1 interacted with ChbHLH42 and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT. In addition, abscisic acid (ABA) treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C. humilis fruit. Interestingly, ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42. Furthermore, ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42. Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C. humilis fruit, deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops.http://www.sciencedirect.com/science/article/pii/S2468014123001450Cerasus humilisAnthocyaninChMYB1FruitABA |
| spellingShingle | Xiaolong Ji Zhe Li Mingyu Zhang Shaoyu Lang Xingshun Song ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis Horticultural Plant Journal Cerasus humilis Anthocyanin ChMYB1 Fruit ABA |
| title | ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis |
| title_full | ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis |
| title_fullStr | ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis |
| title_full_unstemmed | ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis |
| title_short | ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis |
| title_sort | chmyb1 chbhlh42 chttg1 module regulates abscisic acid induced anthocyanin biosynthesis in cerasus humilis |
| topic | Cerasus humilis Anthocyanin ChMYB1 Fruit ABA |
| url | http://www.sciencedirect.com/science/article/pii/S2468014123001450 |
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