Vortex-Assisted Dispersive Molecularly Imprinted Polymer-Based Solid Phase Extraction of Acetaminophen from Water Samples Prior to HPLC-DAD Determination
In the present study, acetaminophen (ACT) molecularly imprinted polymer (ACT-MIP) were successfully synthesized via surface imprinting polymerization. The structural and morphological properties of ACT-MIP were characterized using various analytical techniques. ACT-MIP were used as an adsorbent in a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2297-8739/8/10/194 |
| _version_ | 1797513171681935360 |
|---|---|
| author | Neliswa Mpayipheli Anele Mpupa Philiswa Nosizo Nomngongo |
| author_facet | Neliswa Mpayipheli Anele Mpupa Philiswa Nosizo Nomngongo |
| author_sort | Neliswa Mpayipheli |
| collection | DOAJ |
| description | In the present study, acetaminophen (ACT) molecularly imprinted polymer (ACT-MIP) were successfully synthesized via surface imprinting polymerization. The structural and morphological properties of ACT-MIP were characterized using various analytical techniques. ACT-MIP were used as an adsorbent in a vortex-assisted dispersive molecularly imprinted solid-phase micro-extraction (VA-d-μ-MISPE), coupled with a high-performance liquid chromatography–diode array detector (HPLC-DAD) method for the determination of ACT in water samples. Influential parameters such as the mass of adsorbent, vortex speed, extraction time, desorption volume, and desorption time were optimized using a multivariate approach. Under optimum conditions, the maximum binding capacities of ACT-MIP and NIP (non-imprinted polymers) were 191 mg/g and 71.5 mg/g, respectively. The linearity was attained across concentrations ranging from 0.630 to 500 µg/L, with a coefficient of determination of 0.9959. For ACT-MIP, the limit of detection (LOD) and limit of quantification (LOQ), enhancement factor, and precision of the method were 0.19 ng/L, 0.63 ng/L, 79, and <5%, respectively. The method was applied in the analysis of spiked water samples, and satisfactory percentage recoveries in the range of 95.3–99.8% were obtained. |
| first_indexed | 2024-03-10T06:12:54Z |
| format | Article |
| id | doaj.art-fa40da9861004851bc36944b2a50942f |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T06:12:54Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-fa40da9861004851bc36944b2a50942f2023-11-22T20:00:29ZengMDPI AGSeparations2297-87392021-10-0181019410.3390/separations8100194Vortex-Assisted Dispersive Molecularly Imprinted Polymer-Based Solid Phase Extraction of Acetaminophen from Water Samples Prior to HPLC-DAD DeterminationNeliswa Mpayipheli0Anele Mpupa1Philiswa Nosizo Nomngongo2Department of Chemical Sciences, University of Johannesburg, Doornfontein Campus, P.O. Box 17011, Doornfontein 2028, South AfricaDepartment of Chemical Sciences, University of Johannesburg, Doornfontein Campus, P.O. Box 17011, Doornfontein 2028, South AfricaDepartment of Chemical Sciences, University of Johannesburg, Doornfontein Campus, P.O. Box 17011, Doornfontein 2028, South AfricaIn the present study, acetaminophen (ACT) molecularly imprinted polymer (ACT-MIP) were successfully synthesized via surface imprinting polymerization. The structural and morphological properties of ACT-MIP were characterized using various analytical techniques. ACT-MIP were used as an adsorbent in a vortex-assisted dispersive molecularly imprinted solid-phase micro-extraction (VA-d-μ-MISPE), coupled with a high-performance liquid chromatography–diode array detector (HPLC-DAD) method for the determination of ACT in water samples. Influential parameters such as the mass of adsorbent, vortex speed, extraction time, desorption volume, and desorption time were optimized using a multivariate approach. Under optimum conditions, the maximum binding capacities of ACT-MIP and NIP (non-imprinted polymers) were 191 mg/g and 71.5 mg/g, respectively. The linearity was attained across concentrations ranging from 0.630 to 500 µg/L, with a coefficient of determination of 0.9959. For ACT-MIP, the limit of detection (LOD) and limit of quantification (LOQ), enhancement factor, and precision of the method were 0.19 ng/L, 0.63 ng/L, 79, and <5%, respectively. The method was applied in the analysis of spiked water samples, and satisfactory percentage recoveries in the range of 95.3–99.8% were obtained.https://www.mdpi.com/2297-8739/8/10/194acetaminophenmolecularly imprinted polymerselectivityScatchard analysisadsorption isothermssolid-phase microextraction |
| spellingShingle | Neliswa Mpayipheli Anele Mpupa Philiswa Nosizo Nomngongo Vortex-Assisted Dispersive Molecularly Imprinted Polymer-Based Solid Phase Extraction of Acetaminophen from Water Samples Prior to HPLC-DAD Determination Separations acetaminophen molecularly imprinted polymer selectivity Scatchard analysis adsorption isotherms solid-phase microextraction |
| title | Vortex-Assisted Dispersive Molecularly Imprinted Polymer-Based Solid Phase Extraction of Acetaminophen from Water Samples Prior to HPLC-DAD Determination |
| title_full | Vortex-Assisted Dispersive Molecularly Imprinted Polymer-Based Solid Phase Extraction of Acetaminophen from Water Samples Prior to HPLC-DAD Determination |
| title_fullStr | Vortex-Assisted Dispersive Molecularly Imprinted Polymer-Based Solid Phase Extraction of Acetaminophen from Water Samples Prior to HPLC-DAD Determination |
| title_full_unstemmed | Vortex-Assisted Dispersive Molecularly Imprinted Polymer-Based Solid Phase Extraction of Acetaminophen from Water Samples Prior to HPLC-DAD Determination |
| title_short | Vortex-Assisted Dispersive Molecularly Imprinted Polymer-Based Solid Phase Extraction of Acetaminophen from Water Samples Prior to HPLC-DAD Determination |
| title_sort | vortex assisted dispersive molecularly imprinted polymer based solid phase extraction of acetaminophen from water samples prior to hplc dad determination |
| topic | acetaminophen molecularly imprinted polymer selectivity Scatchard analysis adsorption isotherms solid-phase microextraction |
| url | https://www.mdpi.com/2297-8739/8/10/194 |
| work_keys_str_mv | AT neliswampayipheli vortexassisteddispersivemolecularlyimprintedpolymerbasedsolidphaseextractionofacetaminophenfromwatersamplespriortohplcdaddetermination AT anelempupa vortexassisteddispersivemolecularlyimprintedpolymerbasedsolidphaseextractionofacetaminophenfromwatersamplespriortohplcdaddetermination AT philiswanosizonomngongo vortexassisteddispersivemolecularlyimprintedpolymerbasedsolidphaseextractionofacetaminophenfromwatersamplespriortohplcdaddetermination |