Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers
Abstract The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2017-10-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017005026102&lng=en&tlng=en |
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| author | Boris Briñez Juliana Morini Küpper Cardoso Perseguini Juliana Santa Rosa Denis Bassi João Guilherme Ribeiro Gonçalves Caléo Almeida Jean Fausto de Carvalho Paulino Matthew Ward Blair Alisson Fernando Chioratto Sérgio Augusto Morais Carbonell Paula Arielle Mendes Ribeiro Valdisser Rosana Pereira Vianello Luciana Lasry Benchimol-Reis |
| author_facet | Boris Briñez Juliana Morini Küpper Cardoso Perseguini Juliana Santa Rosa Denis Bassi João Guilherme Ribeiro Gonçalves Caléo Almeida Jean Fausto de Carvalho Paulino Matthew Ward Blair Alisson Fernando Chioratto Sérgio Augusto Morais Carbonell Paula Arielle Mendes Ribeiro Valdisser Rosana Pereira Vianello Luciana Lasry Benchimol-Reis |
| author_sort | Boris Briñez |
| collection | DOAJ |
| description | Abstract The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in order to assess the factors that determine the magnitude of drought response in common beans. A total of 438 polymorphic markers were used to genotype the F8 mapping population. Phenotyping was done in two greenhouses, one used to simulate drought and the other to simulate irrigated conditions. Fourteen traits associated with drought tolerance were measured to identify the quantitative trait loci (QTLs). The map was constructed with 331 markers that covered all 11 chromosomes and had a total length of 1515 cM. Twenty-two QTLs were discovered for chlorophyll, leaf and stem fresh biomass, leaf biomass dry weight, leaf temperature, number of pods per plant, number of seeds per plant, seed weight, days to flowering, dry pod weight and total yield under well-watered and drought (stress) conditions. All the QTLs detected under drought conditions showed positive effects of the SEA 5 allele. This study provides a better understanding of the genetic inheritance of drought tolerance in common bean. |
| first_indexed | 2024-12-14T10:25:11Z |
| format | Article |
| id | doaj.art-fa46faedb906445391d74eb3b1314a89 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-14T10:25:11Z |
| publishDate | 2017-10-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-fa46faedb906445391d74eb3b1314a892022-12-21T23:06:24ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852017-10-01010.1590/1678-4685-gmb-2016-0222S1415-47572017005026102Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markersBoris BriñezJuliana Morini Küpper Cardoso PerseguiniJuliana Santa RosaDenis BassiJoão Guilherme Ribeiro GonçalvesCaléo AlmeidaJean Fausto de Carvalho PaulinoMatthew Ward BlairAlisson Fernando ChiorattoSérgio Augusto Morais CarbonellPaula Arielle Mendes Ribeiro ValdisserRosana Pereira VianelloLuciana Lasry Benchimol-ReisAbstract The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in order to assess the factors that determine the magnitude of drought response in common beans. A total of 438 polymorphic markers were used to genotype the F8 mapping population. Phenotyping was done in two greenhouses, one used to simulate drought and the other to simulate irrigated conditions. Fourteen traits associated with drought tolerance were measured to identify the quantitative trait loci (QTLs). The map was constructed with 331 markers that covered all 11 chromosomes and had a total length of 1515 cM. Twenty-two QTLs were discovered for chlorophyll, leaf and stem fresh biomass, leaf biomass dry weight, leaf temperature, number of pods per plant, number of seeds per plant, seed weight, days to flowering, dry pod weight and total yield under well-watered and drought (stress) conditions. All the QTLs detected under drought conditions showed positive effects of the SEA 5 allele. This study provides a better understanding of the genetic inheritance of drought tolerance in common bean.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017005026102&lng=en&tlng=enabiotic stressinterpopulation gene-poolmolecular markersQTL mappingwater deficit |
| spellingShingle | Boris Briñez Juliana Morini Küpper Cardoso Perseguini Juliana Santa Rosa Denis Bassi João Guilherme Ribeiro Gonçalves Caléo Almeida Jean Fausto de Carvalho Paulino Matthew Ward Blair Alisson Fernando Chioratto Sérgio Augusto Morais Carbonell Paula Arielle Mendes Ribeiro Valdisser Rosana Pereira Vianello Luciana Lasry Benchimol-Reis Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers Genetics and Molecular Biology abiotic stress interpopulation gene-pool molecular markers QTL mapping water deficit |
| title | Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers |
| title_full | Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers |
| title_fullStr | Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers |
| title_full_unstemmed | Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers |
| title_short | Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers |
| title_sort | mapping qtls for drought tolerance in a sea 5 x and 277 common bean cross with ssrs and snp markers |
| topic | abiotic stress interpopulation gene-pool molecular markers QTL mapping water deficit |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017005026102&lng=en&tlng=en |
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