Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury
Abstract Background Evidence has suggested that human adipose-derived stem cells (hADSCs) and low-level laser has neuroprotective effects on spinal cord injury (SCI). Therefore, the combined effect of the hADSCs and laser on neuregeneration and neuropathic pain after SCI was investigated. Methods Fo...
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | http://link.springer.com/article/10.1186/s13287-019-1269-y |
| _version_ | 1818256571624325120 |
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| author | Arash Sarveazad Atousa Janzadeh Gholamreza Taheripak Sima Dameni Mahmoud Yousefifard Farinaz Nasirinezhad |
| author_facet | Arash Sarveazad Atousa Janzadeh Gholamreza Taheripak Sima Dameni Mahmoud Yousefifard Farinaz Nasirinezhad |
| author_sort | Arash Sarveazad |
| collection | DOAJ |
| description | Abstract Background Evidence has suggested that human adipose-derived stem cells (hADSCs) and low-level laser has neuroprotective effects on spinal cord injury (SCI). Therefore, the combined effect of the hADSCs and laser on neuregeneration and neuropathic pain after SCI was investigated. Methods Forty-eight adult male Wistar rats with 200–250 g weight were used. Thirty minutes after compression, injury with laser was irritated, and 1 week following SCI, about 1 × 106 cells were transplanted into the spinal cord. Motor function and neuropathic pain were assessed weekly. Molecular and histological studies were done at the end of the fourth week. Results The combined application of hADSCs and laser has significantly improved motor function recovery (p = 0.0001), hyperalgesia (p < 0.05), and allodynia (p < 0.05). GDNF mRNA expression was significantly increased in hADSCs and laser+hADSC-treated animals (p < 0.001). Finally, co-administration of hADSCs and laser has enhanced the number of axons around cavity more than other treatments (p < 0.001). Conclusions The results showed that the combination of laser and ADSCs could significantly improve the motor function and alleviate SCI-induced allodynia and hyperalgesia. Therefore, using a combination of laser and hADSCs in future experimental and translational clinical studies is suggested. |
| first_indexed | 2024-12-12T17:29:52Z |
| format | Article |
| id | doaj.art-fa49bc7f5c214355b2b7dd89a89614c2 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-12T17:29:52Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-fa49bc7f5c214355b2b7dd89a89614c22022-12-22T00:17:26ZengBMCStem Cell Research & Therapy1757-65122019-06-0110111510.1186/s13287-019-1269-yCo-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injuryArash Sarveazad0Atousa Janzadeh1Gholamreza Taheripak2Sima Dameni3Mahmoud Yousefifard4Farinaz Nasirinezhad5Colorectal Research Center, Iran University of Medical SciencesRadiation Biology Research Center, Iran University of Medical SciencesDepartment of Biochemistry, Faculty of Medicine, Iran University of Medical SciencesPhysiology Research Center, Faculty of Medicine, Iran University of Medical SciencesPhysiology Research Center, Faculty of Medicine, Iran University of Medical SciencesPhysiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical SciencesAbstract Background Evidence has suggested that human adipose-derived stem cells (hADSCs) and low-level laser has neuroprotective effects on spinal cord injury (SCI). Therefore, the combined effect of the hADSCs and laser on neuregeneration and neuropathic pain after SCI was investigated. Methods Forty-eight adult male Wistar rats with 200–250 g weight were used. Thirty minutes after compression, injury with laser was irritated, and 1 week following SCI, about 1 × 106 cells were transplanted into the spinal cord. Motor function and neuropathic pain were assessed weekly. Molecular and histological studies were done at the end of the fourth week. Results The combined application of hADSCs and laser has significantly improved motor function recovery (p = 0.0001), hyperalgesia (p < 0.05), and allodynia (p < 0.05). GDNF mRNA expression was significantly increased in hADSCs and laser+hADSC-treated animals (p < 0.001). Finally, co-administration of hADSCs and laser has enhanced the number of axons around cavity more than other treatments (p < 0.001). Conclusions The results showed that the combination of laser and ADSCs could significantly improve the motor function and alleviate SCI-induced allodynia and hyperalgesia. Therefore, using a combination of laser and hADSCs in future experimental and translational clinical studies is suggested.http://link.springer.com/article/10.1186/s13287-019-1269-yGSK3 betaNeurodegenerationNeuropathic painHyperalgesiaAllodyniaSpinal cord injury |
| spellingShingle | Arash Sarveazad Atousa Janzadeh Gholamreza Taheripak Sima Dameni Mahmoud Yousefifard Farinaz Nasirinezhad Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury Stem Cell Research & Therapy GSK3 beta Neurodegeneration Neuropathic pain Hyperalgesia Allodynia Spinal cord injury |
| title | Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury |
| title_full | Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury |
| title_fullStr | Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury |
| title_full_unstemmed | Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury |
| title_short | Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury |
| title_sort | co administration of human adipose derived stem cells and low level laser to alleviate neuropathic pain after experimental spinal cord injury |
| topic | GSK3 beta Neurodegeneration Neuropathic pain Hyperalgesia Allodynia Spinal cord injury |
| url | http://link.springer.com/article/10.1186/s13287-019-1269-y |
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