The antihemostatic profile of vitamin C: Mechanisms that underlie the technical application of a physiological molecule
The potential of antioxidants as tools for lowering the incidence of diseases, including cardiovascular events, is of growing interest. Some antioxidants (e.g. vitamin E and acetyl-salicylic acid) have been described as effective on cardiovascular diseases with mechanisms that differ from...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600024S.pdf |
| _version_ | 1818701682101452800 |
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| author | Sathler Plínio C. Lourenço André L. Saito Max S. Arêas Ana P.G. Rodrigues Carlos R. Cabral Lúcio M. Castro Helena C. Kang Hye C. |
| author_facet | Sathler Plínio C. Lourenço André L. Saito Max S. Arêas Ana P.G. Rodrigues Carlos R. Cabral Lúcio M. Castro Helena C. Kang Hye C. |
| author_sort | Sathler Plínio C. |
| collection | DOAJ |
| description | The potential of antioxidants as tools for lowering the incidence of
diseases, including cardiovascular events, is of growing interest. Some
antioxidants (e.g. vitamin E and acetyl-salicylic acid) have been described
as effective on cardiovascular diseases with mechanisms that differ from
other scavenging agents. Currently, vitamin C is used to open occluded
long-term central venous catheters, which avoids the process of reinserting
a new one and injuring the patient. In this work, we investigated the
vitamin C antihemostatic profile by evaluating its effects on the
coagulation process. We used different assays, including prothrombin time
(PT), activated partial thromboplastin time (aPTT), thrombin time (TT) and
ancrod time tests. We also examined the overall pH disturbance caused by
vitamin C at different concentrations and its effect on the
thrombin-initiated fibrin polymerization assay. Our results revealed a
significant anticoagulant activity of vitamin C at high plasma
concentrations (surpassing the normal 100 μmol/L ratio) in a
cell-independent mechanism. Our results suggest that vitamin C may affect
blood coagulation by a direct impairment of fibrin assembly and further
formation of a cohesive clot microstructure. This study supports the
literature that points to the antihemostatic ability of antioxidant agents,
and clarifies the mechanism of vitamin C in opening occluded long-term
central venous catheters. |
| first_indexed | 2024-12-17T15:24:43Z |
| format | Article |
| id | doaj.art-fa4fe59508e743498b66b5c7617aef7c |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-17T15:24:43Z |
| publishDate | 2016-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-fa4fe59508e743498b66b5c7617aef7c2022-12-21T21:43:18ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392016-01-0168232533110.2298/ABS150413024S0354-46641600024SThe antihemostatic profile of vitamin C: Mechanisms that underlie the technical application of a physiological moleculeSathler Plínio C.0Lourenço André L.1Saito Max S.2Arêas Ana P.G.3Rodrigues Carlos R.4Cabral Lúcio M.5Castro Helena C.6Kang Hye C.7Universidade Federal Fluminense, Hospital Universitário Antônio Pedro, Programa de Pós-Graduação Patologia, Centro, CEP, Niterói, Brazil + Universidade Federal do Rio de Janeiro, Faculdade de Farmácia, Cidade Universitária, CEP, Rio de Janeiro, BrazilUniversidade Federal Fluminense, Hospital Universitário Antônio Pedro, Programa de Pós-Graduação Patologia, Centro, CEP, Niterói, BrazilUniversidade Federal Fluminense, Hospital Universitário Antônio Pedro, Programa de Pós-Graduação Patologia, Centro, CEP, Niterói, BrazilUniversidade Federal Fluminense, Hospital Universitário Antônio Pedro, Programa de Pós-Graduação Patologia, Centro, CEP, Niterói, BrazilUniversidade Federal do Rio de Janeiro, Faculdade de Farmácia, Cidade Universitária, CEP, Rio de Janeiro, BrazilUniversidade Federal do Rio de Janeiro, Faculdade de Farmácia, Cidade Universitária, CEP, Rio de Janeiro, BrazilUniversidade Federal Fluminense, Hospital Universitário Antônio Pedro, Programa de Pós-Graduação Patologia, Centro, CEP, Niterói, BrazilUniversidade Federal Fluminense, Hospital Universitário Antônio Pedro, Programa de Pós-Graduação Patologia, Centro, CEP, Niterói, BrazilThe potential of antioxidants as tools for lowering the incidence of diseases, including cardiovascular events, is of growing interest. Some antioxidants (e.g. vitamin E and acetyl-salicylic acid) have been described as effective on cardiovascular diseases with mechanisms that differ from other scavenging agents. Currently, vitamin C is used to open occluded long-term central venous catheters, which avoids the process of reinserting a new one and injuring the patient. In this work, we investigated the vitamin C antihemostatic profile by evaluating its effects on the coagulation process. We used different assays, including prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT) and ancrod time tests. We also examined the overall pH disturbance caused by vitamin C at different concentrations and its effect on the thrombin-initiated fibrin polymerization assay. Our results revealed a significant anticoagulant activity of vitamin C at high plasma concentrations (surpassing the normal 100 μmol/L ratio) in a cell-independent mechanism. Our results suggest that vitamin C may affect blood coagulation by a direct impairment of fibrin assembly and further formation of a cohesive clot microstructure. This study supports the literature that points to the antihemostatic ability of antioxidant agents, and clarifies the mechanism of vitamin C in opening occluded long-term central venous catheters.http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600024S.pdfVitamin Cascorbic acidantioxidantbloodcoagulation |
| spellingShingle | Sathler Plínio C. Lourenço André L. Saito Max S. Arêas Ana P.G. Rodrigues Carlos R. Cabral Lúcio M. Castro Helena C. Kang Hye C. The antihemostatic profile of vitamin C: Mechanisms that underlie the technical application of a physiological molecule Archives of Biological Sciences Vitamin C ascorbic acid antioxidant blood coagulation |
| title | The antihemostatic profile of vitamin C: Mechanisms that underlie the technical application of a physiological molecule |
| title_full | The antihemostatic profile of vitamin C: Mechanisms that underlie the technical application of a physiological molecule |
| title_fullStr | The antihemostatic profile of vitamin C: Mechanisms that underlie the technical application of a physiological molecule |
| title_full_unstemmed | The antihemostatic profile of vitamin C: Mechanisms that underlie the technical application of a physiological molecule |
| title_short | The antihemostatic profile of vitamin C: Mechanisms that underlie the technical application of a physiological molecule |
| title_sort | antihemostatic profile of vitamin c mechanisms that underlie the technical application of a physiological molecule |
| topic | Vitamin C ascorbic acid antioxidant blood coagulation |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600024S.pdf |
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