Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6
Four oxalate degrading endophytic bacteria were isolated from oxalate rich Colocasia esculenta tubers. Based upon the oxalate oxidase (EC 1.2.3.4) activity produced in nutrient medium, one bacterium was selected and identified as Ochrobactrum intermedium by 16S rDNA sequencing. Studies on effect o...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2018-12-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/production-of-oxalate-oxidase-from-endophytic-ochrobactrum-intermedium-cl6/ |
| Summary: | Four oxalate degrading endophytic bacteria were isolated from oxalate rich Colocasia esculenta tubers. Based upon
the oxalate oxidase (EC 1.2.3.4) activity produced in nutrient medium, one bacterium was selected and identified
as Ochrobactrum intermedium by 16S rDNA sequencing. Studies on effect of nutritional and non-nutritional
parameters showed that oxalate oxidase production is inducible, requires Manganese ions in the medium, and very
low fill-up volume is beneficial. Shake flask fermentation carried out with medium comprising Sucrose, Ammonium
chloride, Sodium oxalate along with basal salts gave 0.5 UmL-1 oxalate oxidase activity and 0.454 Umg-1specific
activity after 65h of fermentation. |
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| ISSN: | 0973-7510 2581-690X |