Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6
Four oxalate degrading endophytic bacteria were isolated from oxalate rich Colocasia esculenta tubers. Based upon the oxalate oxidase (EC 1.2.3.4) activity produced in nutrient medium, one bacterium was selected and identified as Ochrobactrum intermedium by 16S rDNA sequencing. Studies on effect o...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2018-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/production-of-oxalate-oxidase-from-endophytic-ochrobactrum-intermedium-cl6/ |
| _version_ | 1818398148982210560 |
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| author | Kunal Kumar Prasanna D. Belur |
| author_facet | Kunal Kumar Prasanna D. Belur |
| author_sort | Kunal Kumar |
| collection | DOAJ |
| description | Four oxalate degrading endophytic bacteria were isolated from oxalate rich Colocasia esculenta tubers. Based upon
the oxalate oxidase (EC 1.2.3.4) activity produced in nutrient medium, one bacterium was selected and identified
as Ochrobactrum intermedium by 16S rDNA sequencing. Studies on effect of nutritional and non-nutritional
parameters showed that oxalate oxidase production is inducible, requires Manganese ions in the medium, and very
low fill-up volume is beneficial. Shake flask fermentation carried out with medium comprising Sucrose, Ammonium
chloride, Sodium oxalate along with basal salts gave 0.5 UmL-1 oxalate oxidase activity and 0.454 Umg-1specific
activity after 65h of fermentation. |
| first_indexed | 2024-12-14T07:00:11Z |
| format | Article |
| id | doaj.art-fa50393797424a4bb0ef9d5425d0bb05 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-14T07:00:11Z |
| publishDate | 2018-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-fa50393797424a4bb0ef9d5425d0bb052022-12-21T23:12:27ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-12-011242327233610.22207/JPAM.12.4.75Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6Kunal Kumar0Prasanna D. Belur1Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal. Srinivasanagar, Mangalore - 575 025, India.Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal. Srinivasanagar, Mangalore - 575 025, India.Four oxalate degrading endophytic bacteria were isolated from oxalate rich Colocasia esculenta tubers. Based upon the oxalate oxidase (EC 1.2.3.4) activity produced in nutrient medium, one bacterium was selected and identified as Ochrobactrum intermedium by 16S rDNA sequencing. Studies on effect of nutritional and non-nutritional parameters showed that oxalate oxidase production is inducible, requires Manganese ions in the medium, and very low fill-up volume is beneficial. Shake flask fermentation carried out with medium comprising Sucrose, Ammonium chloride, Sodium oxalate along with basal salts gave 0.5 UmL-1 oxalate oxidase activity and 0.454 Umg-1specific activity after 65h of fermentation.https://microbiologyjournal.org/production-of-oxalate-oxidase-from-endophytic-ochrobactrum-intermedium-cl6/colocasia esculentaendophyteochrobactrum intermediumoxalate oxidase |
| spellingShingle | Kunal Kumar Prasanna D. Belur Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6 Journal of Pure and Applied Microbiology colocasia esculenta endophyte ochrobactrum intermedium oxalate oxidase |
| title | Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6 |
| title_full | Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6 |
| title_fullStr | Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6 |
| title_full_unstemmed | Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6 |
| title_short | Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6 |
| title_sort | production of oxalate oxidase from endophytic ochrobactrum intermedium cl6 |
| topic | colocasia esculenta endophyte ochrobactrum intermedium oxalate oxidase |
| url | https://microbiologyjournal.org/production-of-oxalate-oxidase-from-endophytic-ochrobactrum-intermedium-cl6/ |
| work_keys_str_mv | AT kunalkumar productionofoxalateoxidasefromendophyticochrobactrumintermediumcl6 AT prasannadbelur productionofoxalateoxidasefromendophyticochrobactrumintermediumcl6 |